Effects of Salt Stress and Inoculation Ratios in Cell Cultures of Rubia tinctorum L.

In this study, salt stress was implemented to callus and cell suspension cultures of Rubia tinctorum L. at 100 mM and 200 mM concentrations and the effects on fresh and dry weights (g), relative dry weight ratios, and alizarin and purpurin contents (mg/g dry weight) of biomass were determined. Due to an increase in salt concentrations, biomass obtained from callus and cell suspension cultures were decreased. In callus cultures, due to the low viability and proliferation rate of the callus cells, the biomass showed no significant increase. Alizarin and purpurin contents decreased as the salt concentrations in callus cultures increased, but amounts of these compounds increased in cell suspension cultures. Inoculation ratios were also found effective on fresh and dry weights, and alizarin and purpurin contents of the samples tested. 1:2 inoculation ratio caused higher alizarin and purpurin contents in cell suspension cultures, but biomass amounts were found higher at 1:4 inoculation ratio. The highest alizarin (3.183 mg/g) and purpurin (1.153 mg/g) contents were detected in the sample that had 1:2 inoculation ratios and was obtained from the medium at 200 mM NaCl concentration.

Rubia tinctorum L. Hücre Kültürlerinde Tuz Stresi ve Inokülasyon Oranlarının Etkileri

Bu çalışmada, Rubia tinctorum L. kallus ve hücre süspansiyon kültürlerine 100 mM ve 200 mM konsantrasyonlarda tuz stresi uygulanarak elde edilen biyokütlelerdeki yaş ve kuru ağırlıklar (g), bağıl kuru ağırlık oranları (mg/g kuru ağırlık) ve alizarin ve purpurin içerikleri üzerine etkileri belirlenmiştir. Tuz konsantrasyonundaki artışa bağlı olarak, kallus ve hücre süspansiyon kültürlerinden elde edilen biyokütle azalmıştır. Kallus kültürlerindeki hücrelerde düşük canlılık ve çoğalma oranına rağmen, biyokütle belirgin bir düşüş göstermemiştir. Kallus kültürlerinde tuz konsantrasyonu arttıkça alizarin ve purpurin içeriği azalmıştır, ancak bu bileşiklerin miktarı hücre süspansiyon kültürlerinde artmıştır. Yapılan denemelerde inokülasyon oranlarının yaş ve kuru ağırlık miktarı ve alizarin ve purpurin içeriği üzerine etkili olduğu tespit edilmiştir. Hücre süspansiyon kültürlerinde 1:2 inokülasyon oranı daha yüksek alizarin ve purpurin içeriği sağlarken, 1:4 inoküsyon oranında biyokütle miktarı daha fazladır. En yüksek alizarin (3.183 mg/g) ve purpurin (1.153 mg/g) içerikleri 1:2 inokülasyon oranında ve 200 mM NaCl içeren besin ortamından tespit edilmiştir.

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