Sevinç YANAR, Elvan ŞAHİN, Burçin ASUTAY, Ahmet ÖZBEK, Fikrettin ŞAHİN

Geleneksel Kullanıma Sahip Bitkisel Bir Yağ Karışımının Yara İyileşmesine Etkileri ve Antiviral Aktivitesi

Amaç: Modern tıp yöntemlerine ek olarak kullanılan tedavi yöntemlerini tanımlayan Geleneksel, Tamamlayıcı ve Alternatif tıp (GTAT) kullanımında son yıllarda dünya genelinde bir artış meydana gelmiştir. En sık kullanılan GTAT yöntemlerinden biri olan bitkisel tıp dünya nüfusunun yaklaşık yüzde sekseni tarafından çeşitli rahatsızlıkların tedavisinde kullanılmaktadır. Bu çalışmada, geleneksel tıpta çok yönlü etkileriyle bilinen Olea europaea, Nigella sativa ve Rosmarinus officinalis bitkilerinden özel olarak hazırlanan, Orta ve Doğu Anadolu’da yüzyıllardır kullanılan bir yağ karışımın antiviral etkisinin ve yara iyileşme sürecine katkısının araştırılması amaçlanmıştır. Gereç ve Yöntemler: Çalışmanın ilk aşamasında, yağ karışımının hücrelere toksik olmayan dozu MTS testi ile belirlendi. Belirlenen dozda yağ karışımının in vitro scratch (çizik) testi kullanılarak yara iyileşmesine etkisini incelendi. Bunun için, kültüre edilmiş keratinosit hücre tabakasında çizik oluşturulduktan sonra yağ kombinasyonu uygulandı ve farklı zaman aralıklarında hücrelerin fotoğrafları çekildi. Yağ karışımının antiviral etkinliği ise Poliovirüs tip 1 ve Adenovirüs tip 5 kullanılarak araştırıldı. Virüs titresi Spearman-Karber metodu kullanılarak hesaplandı. Bulgular: Toksik etkisi olmadığı belirlenen %0,005’lik yağ kombinasyonu uygulanan grupta yara kapanma hızının kontrol grubu ile karşılaştırıldığında 12., 24. ve 36. saat sonunda istatistiksel olarak önemli derecede fazla olduğu tespit edildi. Antiviral etkinlik analizi sonucunda yağ karışımının virüs titresinde en az dört log azalmaya neden olarak Poliovirüs tip 1 ve Adenovirüs tip 5 virüslerine karşı oldukça etkili olduğu ortaya kondu. Sonuç: Çalışma sonuçları yağ karışımının yara iyileşmesini hızlandırıcı etkisi olduğunu ve antiviral aktiviteye sahip olduğunu göstermiştir.

Anahtar Kelimeler:

Olea europaea, Nigella sativa, Rosmarinus officinalis, antiviral, yara iyileştirici

Antiviral Activity and Wound Healing Potential of a Traditionally Used Herbal Oil Blend

Objective: Complementary and alternative medicine (CAM) has an increasing usage in the last decades throughout the world. Herbal medicine, the most preferred CAM, is used in the treatment of various disorders by approximately eighty percent of the world's population. In this study, it was aimed to determine the antiviral activity and wound healing potential of a special herbal oil blend prepared from Olea europaea, Nigella sativa and Rosmarinus officinalis that has been used for centuries in Middle and East Anatolia. Materials and Methods: The nontoxic concentration of herbal blend was determined by MTS assay. This concentration was investigated for its wound healing potential using in vitro scratch assay. A scratch was made on cultured keratinocyte cell layer and the herbal blend was added to medium. Pictures of cells were taken at different time points. The antiviral activity was determined using Adenovirus type 5 and Poliovirus type 1. Virus titer was calculated by Spearman-Karber method. Results: The nontoxic concentration of the blend was found to show statistically higher wound healing rate in compare to control group at the end of 12, 24 and 36 hours. According to antiviral efficacy test, four log of reduction in virus titer was seen, which meant that the mixture was quite effective against the viruses used in the study. Conclusion: The study shows that the special herbal blend speed up wound healing rate and it also has antiviral activity.

Keywords:

Olea europaea, Nigella sativa, Rosmarinus officinalis, antiviral, wound healing,

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