Mustafa Sencer KARAGÜL, Mehmet Engin MALAL, Kadir AKAR

Düzce İlinde Koyunlarda Coxiella burnetii ve Chlamydia abortus Etkenlerine karşı Antikorların Araştırılması

Amaç: Bu çalışmanın amacı Düzce ilindeki koyunlarda, Coxiella burnetii ve Chlamydia abortus etkenine karşı spesifik antikorları araştırmaktır. Q humması ve koyunların enzootik abortusu hastalıklarının ayrı ayrı nedeni olan söz konusu 2 patojen, küçük ruminantlarda yavru atmalar ile ilişkilidir. Mikrobiyal etkenlerin neden olduğu yavru atmalara bağlı ekonomik kayıplar dünyanın birçok ülkesinde kayda değer seviyededir.Gereç ve Yöntemler: Çalışma kapsamında serolojik testler için koyun kan örnekleri Düzce ilinden toplandı. Ticari ELISA kitleri, C. burnetii ve C. abortus etkenlerine karşı spesifik antikorların tespiti için kullanıldı. Düzce ilinin 5 ilçesindeki 12 köy veya mahallede yer alan 14 sürüden alınmış 72 numune çalışmada kullanıldı. Numuneler, 2-5 yaş aralığında yavru atma geçmişi olmayan dişi hayvanlardan alındı.Bulgular: Sırasıyla koyunların enzootik abortusu ve Q humması hastalıkları için total seroprevalans %20,83 ve %26,38 olarak bulundu. Fakat seropozitif sürü oranı, total seroprevalansdan daha yüksek oranda sırasıyla koyunların enzootik abortusu ve Q humması hastalıkları için %42,8 ve %50 olarak bulundu. 14 sürü içinde 7 sürüde her iki hastalık için seropozitif hayvanlar tespit edildi. Sonuç: Çalışma sonuçları Düzce ilindeki araştırılan koyun sürülerinin C. burnetii ve C. abortus etkenlerine maruz kaldığını doğrulamaktadır. Elde edilen verilere göre, Düzce ilinde koyun yavru atma vakalarında çalışmada araştırılan iki abort etkenin muhtemel bağlantısının değerlendirilmesi faydalı olacaktır.

Anahtar Kelimeler:

Abort, Chlamydia, Coxiella burnetii, seroprevalans, Q humması

Investigation of Coxiella burnetii and Chlamydia abortus Antibodies in Sheep in Düzce Region

Aim: The aim of this study is to investigate the Coxiella burnetii and Chlamydia abortus antibodies in sheep in Düzce province. These two pathogens are causative agents of Query fever and Enzootic abortion of ewes, respectively which are related to abortion in small ruminants. Economic loss due to the abortions which are caused by microbiological agents is considerably high in many countries. Material and Methods: Blood samples of sheep were collected from Düzce province of Turkey for serological tests. Commercial enzyme-linked immunosorbent assay (ELISA) was used for the detection of specific antibodies to C. burnetii and C. abortus. A total of 72 samples obtained from 14 herds located in 12 villages/districts of 5 towns in Düzce province were utilized in this study. Samples were collected from 2-5-year-old female animals without an abortion history.Results: Total seroprevalence of Enzootic abortion of ewes and Query fever was found to be 20.83% and 26.38%, respectively. However, the proportion of seropositive herds was found to be 42.8% for Enzootic abortion of ewes and 50% for Q fever, which is higher than total seroprevalence. 6 flocks out of 14 were found to be seropositive for both of the diseases simultaneously. Conclusion: This study confirms the exposure of sheep herds in Düzce province to C. burnetii and C. abortus and provides original seroprevalence data about Düzce. The data gathered are beneficial to evaluate the possible connection of two investigated abortogenic pathogens in sheep abortion cases in Düzce province.

Keywords:

Abortion, Chlamydia, Coxiella burnetii, seroprevalence, Q fever,

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