Ayşe BALDEMİR, Nilay ILDIZ, Ülkü KARAMAN, Ahmet Emre EKER, Ufuk İNCE

ARTEMISIA ABSINTHIUM L., A. LUDOVICIANA NUTT. VE A. VULGARIS L. (ASTERACEAE) EKSTRE VE UÇUCU YAĞLARININ KARBAPENEM DİRENÇLİ SUŞLARA KARŞI IN VITRO ANTİBAKTERİYEL AKTİVİTESİNİN ARAŞTIRILMASI

Genel Bilgi: Çoklu ilaç direncine sahip bakterilerin giderek artması antibiyotik etkinliğini ciddi olarak düşürmekte ve terapötik başarısızlık sıklığını artırmaktadır. Son yıllarda araştırmacılar yeni antibakteriyel ilaçların keşfi için doğal bitkisel kaynakları tercih etmektedirler. Dünyada, Artemisia L. cinsi Asteraceae familyasının tanınmış türlerini içermekte olup, karakteristik kokuları veya tatları nedeniyle botanik ve farmasötik bakımdan önem arz etmektedirler. Amaç: Artemisia absinthium L., A. ludoviciana Nutt. ve A. vulgaris L. türlerinin karbapenem dirençli bakterilere karşı antibakteriyel etkinliklerini araştırmaktır. Yöntem: Metanol ekstreleri ve uçucu yağlar sırasıyla 250, 500, 1000, 2000 μg/mL ve 15.625, 31.25, 62.5, 125 μg/mL konsantrasyonlarında hazırlandı. Sıvı mikrodilüsyon testi, bazı Gram negatif standart suşlar (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 35218, Klebsiella pneumoniae ATCC 700630, Acinetobacter baumannii ATCC 19606) ve klinik olarak karbapenem dirençli Gram negatif suşlara (E. coli, K. pneumoniae, P. aeruginosa, Acinetobacter spp.) karşı örneklerin minimum inhibisyon konsantrasyonlarını (MIK) belirlemek için kullanıldı. Bulgular: A. absinthium uçucu yağı standart suşlar ile karbapenem dirençli P. aeruginosa suşu dışında diğer klinik suşlarda (15,62-125 μg/mL) etkili bulunmuştur. Ayrıca A. absinthium uçucu yağı klinik Acinetobacter suşlarına karşı 31,25 μg/mL konsantrasyonda etkili olduğu belirlenmiştir. A. ludoviciana uçucu yağı sırasıyla 15,625 ve 125 μg/mL'de P. aeruginosa ve A. baumannii suşlarına karşı etkili bulunmuştur. Sonuç: Artemisia türlerinin karbapenem dirençli Gram negatif suşların önemli inhibitörleri olabileceği sonucuna ulaşılmıştır.

Anahtar Kelimeler:

Karbapenem direnci, Artemisia, uçucu yağ, metanol ekstre, antibakteriyel aktivite.

Investigation of in Vitro Antibacterial Activity against Carbapenem Resistant Strains Of Artemisia Absinthium L., A. Ludoviciana Nutt. And A. Vulgaris L (Asteraceae) Extract and Essential Oils

Background: The continuous emergence of multi drug resistant bacteria severely reduces the efficacy of antibiotic increases the frequency of therapeutic failure. In recent years, researchers often prefer natural herbal resources for the discovery of new antibacterial drugs. In the world, Artemisia L. genus, are the well-known species of the family Asteraceae, have botanical and pharmaceutical attention due to their characteristic smells or flavors. Objective: The present study researches the antibacterial activities against carbapenem resistant bacteria of Artemisia absinthium L., A. ludoviciana Nutt. and A. vulgaris L. Method: Methanol extracts and essential oils were prepared at concentrations of 250, 500, 1000, 2000 μg/mL; 15.625, 31.25, 62.5, 125 μg/mL respectively. Broth microdilution assay was used to determine minimum inhibitory concentrations (MICs) of samples against some Gram negative standard strains (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 35218, Klebsiella pneumoniae ATCC 700630, Acinetobacter baumannii ATCC 19606) and clinically carbapenem resistant Gram negative bacteria strains (E. coli, K. pneumoniae, P. aeruginosa, Acinetobacter spp.). Results: A. absinthium essential oil had antibacterial effect on standards and clinical strains (15.625-125μg/mL) except carbapenem resistant P. aeruginosa strain. In addition, A. absinthium essential oil was detected antibacterial effect against clinical Acinetobacter spp. at very low (31.25 μg/ mL) concentration. A. ludoviciana essential oil was effective against P. aeruginosa and A. baumannii strains at 15.625 and 125 μg/mL respectively. Conclusion: Artemisia species may be significant inhibitors of carbapenem resistant Gram negative strains.

Keywords:

Carbapenem resistance, Artemisia, essential oil, methanol extract, antibacterial activity,

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