Yabancı İstilacı Yaban Arılarının (Sceliphron curvatum Smith, 1870) Çamur Yuvalarında Bakteriyel Flora Analizi

Yalnız, istilacı bir yaban arısı türü olan Sceliphron curvatum (Smith, 1870) çamurdan yuva yapar. Bu tür Türkiye için yeni bir kayıttır. Bu çalışma, S. curvatum yaban arılarının yaptıkları çamur yuvalarında bakteri florasının belirlenmesi amacıyla yapılmıştır. Ordu ili Perşembe ilçesinden toplanan ergin arıların yaptığı çamur yuvalarından bakteri izolasyonları yapılmıştır. Çalışma sonucunda beş izolat elde edilmiş ve bu izolatlardan üç tanesi tanımlanmıştır. İzolat 1 Staphylococcus vitulinus, izolat 3 Aeromonas sobria ve izolat 4 Pseudomonas aeruginosa olarak belirlenmiştir. İzolatların tanımlama yüzdeleri sırasıyla S. vitulinus için %84, A. sobria için %84 ve P. aeruginosa için %94 olarak tespit edilmiştir. Bu bakteri türlerinin insanlar ve diğer canlılar üzerinde etkili enfeksiyonlara neden olan önemli bakteri türleri olduğu belirlenmiştir.

Anahtar Kelimeler:

çamur yuva yapan yaban arısı, çamur yuva, yabancı istilacı

Bacterial Flora Analysis in Mud Nests of Alien Invasive Wasps (Sceliphron curvatum Smith, 1870)

Sceliphron curvatum (Smith, 1870), a solitary, invasive wasp species, builds a mud nest. This species is a new record for Türkiye. This study was carried out to determine the bacterial flora in mud nests made by S. curvatum wasps. Bacterial isolations were made from mud nests made by adult bees collected from the town of Perşembe in Ordu province. As a result of the study, five isolates were obtained and three of these isolates were identified. Isolate 1 was identified as Staphylococcus vitulinus, isolate 3 Aeromonas sobria and isolate 4 Pseudomonas aeruginosa. Identification percentages of the isolates were determined as 84% for S. vitulinus, 84% for A. sobria and 94% for P. aeruginosa, respectively. It has been determined that these bacterial species are important bacterial species that cause effective infections on humans and other living things.

Keywords:

mud dauber wasp, mud-nest, alien invasive,

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Bailey, L. (1968). Honey bee pathology. Annual Review of Entomology, 13(1), 191–212. https://doi.org/10.1146/annurev.en.13.010168.001203
Barry, A., Gavan, T.L., Badal, R. E., & Telenson, M. J. (1982). Sensitivity, specificity, and reproducibility of the Auto Microbic system (with the Enterobacteriaceae-plus Biochemical Card) for identifying clinical isolates of Gram-negative bacilli. Journal of Clinical Microbiology, 15(4), 582–588. https://doi.org/10.1128/jcm.15.4.582-588.1982
Bitsch, J., & Barbier, Y. (2006). Répartition de l'espèce invasive Sceliphron curvatum (F. Smith) en Europe et plus particulièrement en France (Hymenoptera, Sphecidae). Bulletin de la Société Entomologique de France, 111(2), 227–237. https://doi.org/10.3406/bsef.2010.2838
Bogusch, P., Liška, P., Lukáš, J., & Dudich, A. (2005). Spreading and summary of the knowledge of the invasive sphecid wasp Sceliphron curvatum (Smith 1870) in the Czech Republic and Slovakia (Hymenoptera:nApocrita, Sphecidae). Linzer Biologische Beiträge, 37(1), 215–221. https://doi.org/10.1128/jcm.15.4.582-588.1982
Bogusch, P., Straka, J., & Srba, M. (2004). The sphecid wasp from the Botanical Garden in Prague. Živa, 51(3), 121–122. https://www.zobodat.at/pdf/LBB_0037_1_0215-0221.pdf
Bohart, R. M., Bohart, R. M., & Menke, A. S. (1976). Sphecid wasps of the world: a generic revision. University of California Press.
Diggle, S.,& Whiteley, M (2020). Microbe profile: Pseudomonas aeruginosa: Opportunistic pathogen and lab rat. Microbiology, 166(1), 30–33. https://doi.org/10.1099%2Fmic.0.000860
Ertürk, Ö., & Taş, B. (2022). Distribution and a new locality record of the invasive Asian wasp, Sceliphron curvatum (F. Smith, 1870) (Hymenoptera: Sphecidae) in Europe and Turkey. Pakistan Journal of Zoology, 54(1), 315–321. http://doi.org/10.17582/journal.pjz/20180723220732
Ertürk, Ö., Taş, B., Şahin, H., & Çaldaş, A. (2019). Determination of some structural features of the mud nest materials of Sceliphron curvatum (Smith, 1870), (Hymenoptera: Sphecidae) in Turkey. Journal of the Entomological Research Society, 21(2), 157–166. https://www.entomol.org/journal/index.php/JERS/article/view/1506
Gepp, J., & Bregant, E. (1986). Zur biologie der synanthropen, in Europa eingeschleppten orientalischen mauerwespe Sceliphron (Prosceliphron) curvatum (Smith, 1870) (Hymenoptera: Sphecidae). Mitteilungen des Naturwissenschaftlichen Vereines für Steiermark, 116, 221–240. https://www.zobodat.at/pdf/MittNatVerSt_116_0221-0240.pdf
Gülmez, Y., & Can, İ. (2015). First record of Sceliphron (Hensenia) curvatum (Hymenoptera: Sphecidae) from Turkey with notes on its morphology and biology. North-Western Journal of Zoology, 11(1), 174–177. https://biozoojournals.ro/nwjz/content/v11n1/nwjz_142201_Gulmez.pdf
Hensen, R. V. (1987). Revision of the subgenus Prosceliphron van der Vecht (Hymenoptera, Sphecidae). Tijdschrift voor Entomologie, 129, 217–261. https://www.biodiversitylibrary.org/part/69311
Kohl, F. F. (1918). Die Hautflüglergruppe “Sphecinae”. IV. Die natürliche Gattung Sceliphron Klug (= Pelopaeus Latr.). Annalen des k.k. Naturhistorischen Hofmuseums, 32, 1−171. https://doi.org/10.5962/bhl.part.2204
Martinez-Murcia, A. J., Benlloch, S., & Collins, M. D. (1992). Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. International Journal of Systematic and Evolutionary Microbiology, 42(3), 412–421. https://doi.org/10.1099/00207713-42-3-412
Papadopoulou-Karabela, K., Iliadis, N., Liakos, V., & Bourdzy-Hatzopoulou, E. (1992). Experimental infection of honeybees by Pseudomonas aeruginosa. Apidologie, 23(5), 393–397. https://doi.org/10.1051/apido:19920501
Poinar, G., & Thomas G. (1978). Diagnostic manual for the identification of insect pathogens. Plenum Press.
Remold, S. K., Brown, C. K., Farris, J. E., Hundley, T. C., Perpich, J. A., & Purdy, M. E. (2011). Differential habitat use and niche partitioning by Pseudomonas species in human homes. Microbial Ecology, 62, 505–517. https://doi.org/10.1007/s00248-011-9844-5
Schmid-Egger, C. (2005). Sceliphron curvatum (F. Smith 1870) in Europa mit einem Bestimmungsschlüssel für die europäischen und mediterranen Sceliphron-Arten (Hymenoptera, Sphecidae). BembiX, 19, 7–28. http://bembix.de/publicationen_pdf/Schmid-Egger%202005%20Sceliphron%20curvatum%20in%20Europa.pdf
Stover, C. K., Pham, X. Q., Erwin, A. L., Mizoguchi, S. D., Warrener, P., Hickey, M. J., ... & Olson, M. V. (2000). Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature, 406(6799), 959–964. https://doi.org/10.1038/35023079
Švec, P., Vancanneyt, M., Sedláček, I., Engelbeen, K., Štětina, V., Swings, J., & Petráš, P. (2004). Reclassification of Staphylococcus pulvereri Zakrzewska-Czerwińska et al. 1995 as a later synonym of Staphylococcus vitulinus Webster et al. 1994. International Journal of Systematic and Evolutionary Microbiology, 54(6), 2213–2215. https://doi.org/10.1099/ijs.0.63080-0
Trüper, H. G., & de' Clari, L. (1998). Taxonomic note: erratum and correction of further specific epithets formed as substantives (nouns) 'in apposition'. International Journal of Systematic Bacteriology, 48, 615–616. https://doi.org/10.1099/00207713-48-2-615
Van der Vecht, J., & Van Breugel, F. M. A. (1968). Revision of the nominate subgenus Sceliphron Latreille (Hymenoptera, Sphecidae) (Studies on the Sceliphronini, Part I). Tijdschrift voor Entomologie, 111(1), 185–255. https://www.biodiversitylibrary.org/part/65807
Webster, J. A., Bannerman, T. L., Hubner, R. J., Ballard, D. N., Cole, E. M., Bruce, J. L., ... & Kloos, W. E. (1994). Identification of the Staphylococcus sciuri species group with Eco RI fragments containing rRNA sequences and description of Staphylococcus vitulus sp. nov. International Journal of Systematic and Evolutionary Microbiology, 44(3), 454–460. https://doi.org/10.1099/00207713-44-3-454
Zakrzewska-Czerwińska, J., Gaszewska-Mastalarz, A., Lis, B., Gamian, A., & Mordarski, M. (1995). Staphylococcus pulvereri sp. nov., isolated from human and animal specimens. International Journal of Systematic and Evolutionary Microbiology, 45(1), 169–172. https://doi.org/10.1099/00207713-45-1-169