Hande BALTACIOĞLU, Emine ŞAHİN, Ecem KARADAĞ

ŞEFTALİ POSASINDAN ULTRASON VE MİKRODALGA DESTEKLİ EKSTRAKSİYON YÖNTEMLERİYLE FENOLİK BİLEŞİKLERİN ELDESİ

Bu çalışmada şeftali posasından fenolik maddelerin ekstraksiyonu üzerine 3 farklı yöntemin etkisi toplam fenolik madde (TFM) miktarları ve antioksidan aktiviteleri kıyaslanarak belirlenmiştir. Posadan fenolik bileşikler % 80’lik metanol (% 1 HCl içeren) kullanılarak, geleneksel yöntemle (30, 60, 120 ve 240 dakika); ultrason destekli ekstraksiyonla (% 60, 80 ve 100 genlik; 10, 20 ve 30 dakika); mikrodalga destekli ekstraksiyonla (360, 600 ve 900 W; 30, 60 ve 90 saniye) uygulanarak ekstrakte edilmiştir. Geleneksel yöntemde 4 saat sonunda TFM 1834,88±84,55 mg GAE/kg YA olarak belirlenmiştir. Ultrason destekli ekstraksiyonda TFM %100 genlikte 10 dakika için 1817,21± 36,95 mg GAE/kg YA olarak ölçülmüştür. Mikrodalga destekli ekstraksiyonda TFM, 900 W ve 90 s için 2802,464±61,49 mg GAE/kg YA olarak belirlenmiştir. EC50 değeri, geleneksel ekstraksiyon için 37,54±0,08 mg/ml, ultrason destekli ekstraksiyon için 73,72±0,13 mg/ml ve mikrodalga destekli ekstraksiyon için 63,17±0,16 mg/ml olarak belirlenmiştir. Buna göre mikrodalga ve ultrason destekli ektraksiyonun zaman tasarrufu açısından önemli avantajlar sağladığı söylenebilmektedir.

Anahtar Kelimeler:

Şeftali, Fenolik Bileşikler, Ultrason Destekli Ekstraksiyon, Mikrodalga Destekli Ekstraksiyon

EXTRACTION OF PHENOLIC COMPOUNDS FROM PEACH POMACE BY MICROWAVE AND ULTRASOUND ASSISTED EXTRACTION

In this study, the effect of three different methods on the extraction of phenolic compounds from peach pomace was determined in terms of total phenolic compound (TPC) and antioxidant activity. Phenolic compounds were extracted from peach pomace using 80% methanol (containing 1% HCl) by conventional method (30, 60, 120 and 240 minutes); ultrasound-assisted extraction (60, 80 and 100% amplitude; 10, 20 and 30 min); microwave-assisted extraction (360, 600 and 900 W; 30, 60 and 90 seconds). In the conventional method, TPC was determined as 1834.88±84.55 mg GAE/kg FW after 4 hours. In the ultrasound-assisted extraction, TPC was measured as 1817.21 ± 36.95 mg GAE/kg FW at 100% amplitude for 10 minutes. It was determined that TPC was 2802.464 ± 61.49 mg GAE/kg FW for microwave assisted extraction at 900 W and 90 s. The EC50 value was 37.54 ± 0.08 mg/ml for conventional extraction, 73.72 ± 0.13 mg/ml for ultrasound assisted extraction and 63.17 ± 0.16 mg/ml for microwave assisted extraction. Accordingly, it can be said that microwave and ultrasound assisted extraction provides significant advantages in terms of time saving.

Keywords:

Phenolic compounds, microwave assisted extraction, peach, ultrasound assisted extraction,

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