Ramazan ÖCAL, İlker TAŞÇI, M. Salih DEVECI, Bilgin CÖMERT, Ahmet Turan ISIK, M. Refik MAS

Deneysel Kronik Pankreatitte N-Asetil Sistein Uygulamasinin Pankreatik Fibroz Üzerine Etkisi

Bu çalışmada, N-asetilsisteinin, kronik pankreatit hayvan modelinde fibrozis, atrofi ve oksidatif stres üzerindeki etkisini değerlendirmeyi amaçladık. Kronik pankreatit, ratların ana pankreatik kanallarına 0.4 ml, %2’lik trinitrobenzen sülfanik asit (TNBS) enjeksiyonu ile uyarılır. 60 Sprague-Dawley sıçanı dört gruba randomize edildi; Grup I (n= 15): salin (sham), Grup II (n= 15): fibrozis + N-asetilsistein (NAC), Grup III (n= 15): fibrozis (TNBS), Grup IV: etanol. 8. haftanın sonunda tüm ratlar sakrefiye edildi ve pankreatik dokular, oksidatif stres belirteçleri, fibrozis ve atrofi progresyonları incelendi. NAC grubunda doku malondialdehid (MDA) düzeyleri anlamlı olarak düşük bulunurken (p <0.001); süperoksit dismutaz (SOD) ve glutation peroksidaz (GSH-Px) aktiviteleri TNBS grubuna göre anlamlı olarak yüksek bulundu (sırasıyla p <0.001 ve p <0.001). Dokulardaki histopatolojik incelemede NAC grubunun histopatolojik skorunun TNBS grubundan anlamlı derecede düşük olduğu izlenirken (p<0.003) oksidatif stres ile histopatolojik skordaki artış arasında anlamlı bir ilişki olduğu saptandı (histopatolojik skor/MDA: p<0.03, SOD: p<0.04, GSH-Px: p<0.02). Sonuç olarak, NAC uygulaması kronik pankreatitte oksidatif stresi azaltmakta olup fibrozis ve atrofi üzerinde yararlı etkilere sahiptir.

Anahtar Kelimeler:

Deneysel Kronik Pankreatit, Fibrozis, Hayvan Modeli, N-Asetilsistein

The Effect of N-Acetylcysteine on Fibrosis in Experimental Rat Chronic Pancreatitis

In this study, we aimed to assess the effect of N-acetylcysteine on fibrosis, atrophy and oxidative stress with chronic pancreatitis in an animal model. Chronic pancreatitis is stimulated by injection of 0.4 ml, 2% trinitrobenzene sulfanic acid (TNBS) into major pancreatic ducts of the rats. 60 Sprague-Dawley rats were randomized into four group; Group I (n=15): saline (control), Grup II (n=15): fibrosis + N-acetylcysteine (NAC), Grup III (n=15): fibrosis (TNBS), Grup IV: ethanol. At the end of the 8th week, all rats were sacrificed, and rat pancreatic tissues, oxidative stress markers and progression of fibrosis and atrophy were examined. superoxide dismutase (SOD) and glutation peroxidase (GSH-Px) activities were significantly higher with respect to TNBS group (respectively p<0.001 and p<0.001); whereas tissue malondialdehyde (MDA) levels were found significantly lower in NAC group (p<0.001). Histopathological analysis of the tissues revealed that histopathological score of NAC group was significantly lower than TNBS group (p<0.003), and was found a significant relation between increase in oxidative stress and histopathological score (histopathological score/MDA: p<0.03, SOD: p<0.04, GSH-Px: p<0,02). In conclusion, administration of NAC decreases oxidative stress in chronic pancreatitis and has beneficial effects on fibrosis and atrophy.

Keywords:

Animal Model, Experimental Chronic Pancreatitis, Fibrosis, N-Acetylcysteine,

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