Şima ŞAHİNDURAN, Mehmet KALE, Reyda KIYICI

BVD ve BHV-1 ile tek ve çift infeksiyonlarda bazı akut faz proteinleri ve hepcidin düzeyleri

Bu çalışmanın amacı, ikili (Bovine viral diarrhea virus / Bovine herpesvirus -1) ve tek enfeksiyonlu (Bovine viral diarrhea virus veya Bovine herpesvirus -1) hayvanlarda ve ayrıca sağlıklı sığırlarda serum amiloid A, haptoglobin ve özellikle hepcidin konsantrasyonlarını belirlemekti. Daha önce sadece hepcidin geni üzerinde çalışmalar yapılmış olup ancak sığır hastalıklarında hepcidin değerleri araştırılmamıştır. Bu çalışmada toplam 56 sığır kullanılmıştır. Onlardan 17'inde Bovine herpesvirus-1, 13 tanesinde Bovine viral diarrhea virüsü, bunların 4'ünde ise hem Bovine herpesvirus-1 hem Bovine viral diarrhea virüsü tespit edildi. Kontrol grubu ise 22 sağlıklı sığırdan oluşturuldu. Bulgulara göre, çift infeksiyonlu hayvanlar ile tek enfeksiyonlu hayvanlar arasında akut faz proteinlerinin seviyelerinde belirgin farklılık saptanmamıştır. Serum hepcidin değerleri, 3 çalışma grubu arasında farklılık göstermedi. Fakat üç deneme grubunda kontrol grubuna göre anlamlı farklılıklar vardı (p <0.001). Haptoglobin değerleri hepcidin değerlerine yakın bulundu. Bu çalışma ile ilk defa sağlıklı sığırlarda hepcidin'in normal değerleri saptanmaya çalışıldı. Bu sonuçlara göre, hepcidin, bir akut faz proteini olarak hastalıkların teşhisi için bir marker olarak kullanılabileceği önerilebilir.

Anahtar Kelimeler:

bovine viral diarrhea, ; bovine herpesvirus;, akut faz proteinleri, hepcidin

Some acute phase proteins and hepcidin levels in single and dual infection with BVD and BHV-1

The aims of this study were to determine serum amyloid A, haptoglobin and especially hepcidin concentrations in animals with dual (Bovine viral diarrhea virus / Bovine herpesvirus -1) and single infections ( Bovine viral diarrhea virus or Bovine herpesvirus -1) and reference ranges of hepcidin in healthy clinicaly cattle. Previously, studies have been conducted on only hepcidin gene but hepcidin values has not been studied in cattle diseases. In this study totaly 56 cattle were used. In 17 of them Bovine herpesvirus-1, 13 of them Bovine viral diarrhea virus, 4 of them both Bovine herpesvirus-1 and Bovine viral diarrhea virus were diagnosed. The control group was composed of 22 clinicaly healthy animals. According to the results animals naturally infected with dual infection did not show more significant differences level of acute phase proteins compared to animals with single infection. Serum hepcidin values did not differ between the 3 trial groups. But there were significant differences in three trial groups results as compared to control group (p<0.001). Haptoglobin analysis results were similar to the results of hepcidin analysis. We demonstrated the normal ranges of hepcidin in 22 clinically healthy cows. According to these results, we can suggest that the hepcidin can be used as a marker for the diagnosis of the diseases as an acute phase protein.

Keywords:

bovine viral diarrhea, bovine herpesvirus, acute phase proteins, hepcidin,

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