Aynur BAŞALP, İbrahim HATİPOĞLU, Bertan Koray BALCIOĞLU, İbrahim SÖĞÜT, Aylin ÖZDEMİR BAHADİR, Kamil Serkan UZYOL, Hülya SİVAS, Berrin ERDAĞ

Filamentöz faj üzerinde eksperese edilen Hepatit B yüzey antijenine karşı antikor yanıtı

Fajlar organizmada humoral ve hücresel immün yanıtı uyandırabilen yüksek immünojenik özelliğe sahip moleküllerdir ve potansiyel aşı taşıyıcısı olarak kullanılmaktadırlar. Bu çalışmada Hepatit B yüzey antijeni (HBsAg) tam molekül olarak M13 faj yüzeyinde füzyon protein olarak ekprese edilmiş ve rekombinant fajın antijenik etkisi BALB/cJ farelerinde incelenmiştir. Farelerin yüksek dozda (1012) rekombinant faj ile immünize edilmesinin normal faja kıyasla daha yüksek antikor yanıtına neden olduğu gösterilmiştir. BALB/cJ farelerinde elde edilen olumlu yanıt sonrası rekombinant fajın etkinliği Hepatit B virüsü (HBV) transgenik farelerde ve hibrid farelerde (C57BL/6JXBALB/c) incelenmiş ve ayrıca etkisi alüminyum adjuvantı ve mineral yağ adjuvantı gibi yaygın kullanımı olan adjuvantlarla karşılaştırılmıştır. Çalışmanın sonuçları BALB/cJ farelerin, HBV transgenik farelerin ve hibrid farelerin rekombinant faj ile immünizasyonunun antijene özgü antikor yanıtı oluşturduğunu göstermiştir. Sonuçlar ayrıca HBV taşıyıcılarında immün yanıt oluşturmak için güçlü adjuvantların kullanılması gerektiğini de işaret etmektedir.

Anahtar Kelimeler:

Adjuvant, Antikor yanıtı, Hepatit B virüsü, Faj gösterim

Antibody response to Hepatitis B surface antigen displayed on filamentous phage

Phages are highly immunogenic molecules capable of inducing both humoral and cellular immune response and have been used as potential vaccine delivery vehicles. In this study, the full-length Hepatitis B surface antigen (HBsAg) was expressed as HBsAg-pIII fusion protein on M13 phage and the efficiacy of recombinant phage was studied in BALB/cJ mice. It was shown that, immunization of mice with the highest dose of recombinant phage (1012) generated an increased antibody response compared to wild type phage immunization. After having a promising result in BALB/cJ mice, recombinant phage was tested in Hepatitis B virus (HBV) transgenic mice and hybrid mice (C57BL/6JXBALB/c) and its effect was also compared with the well known adjuvants such as alum and mineral oil adjuvants. Our results have shown that immunization of BALB/cJ mice, HBV transgenic mice and hybrid mice with recombinant phage elicited an antigen-specific antibody response. The results also indicated that very strong adjuvants/carriers should be used to induce immune response in HBV carriers.

Keywords:

Adjuvant, Antibody response, Hepatitis B Virus, Phage display,

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