Bayram METİN, Esma MENEVŞE, Abdullah SİVRİKAYA, Tamer ALTİNOKS, Hilal ARIKOĞLU

The effects of ankaferd blood stopper on DNA damage and enzymes with paranchymal damaged rabbits

Ankaferd blood stopper (ABS) is a medical product that is used in several injuries, dental operations, prevention of minor or major bleeding after spontaneous or surgerical operations and have anti-microbial, anti-inflamatory, anti-thrombin, anti-platelet, anti-atherosclerotic, anti-oxidants effects. The present study is aimed to evaluate the effects of ABS on 8-hydroxy-2ˈ-deoxyguanosine (8-OHdG), superoxide dismutase (SOD), myeloperoxidase (MPO) levels over pleural adhesions in rabbits with pulmonary parenchymal damage.16 New Zelland species rabbits were divided in two groups such as control (n=7) and study group (n=7). One rabbit in each group died during anesthesia. In both groups, we performed wedge resections in equal size to the left lower lobes of all rabbits. No interventions were made on control group, whereas 5 puff’s (1 cc) ABS was performed to the resection area at study group. Tube thoracostomy that performed both groups were terminated postoperatively at 6th hour after drainage and air leakages follow up. Rabbits were sacrificed with anesthetics at postoperative 8th day. Lung tissues were collected for analyzing of 8-OHdG, SOD, MPO. The 8-OHdG levels were respectively 2.01±0.39 ng/ml in control group and, 0.38±0.12 ng/ml in study. The differences between study and control group were statistically important group (p

PDF

___

1. Turhan N, Bilgili H, Captug O, Kurt M, Shorbagi A, Beyazit Y, Kurt OK, Kosar A, Haznedaroglu IC. Evaluation of a haemostatic agent in rabbits. Afr J Tradit Complement Altern Med. 2011;8(1):61-5.

2. Göker H, Haznederoglu IC, Ercetin S, Kirazli S, Akman U, Ozturk Y, Firat HC. Haemostatic actions of the folkloric medicinal plant extract Ankaferd Blood Stopper. J Int Med Res. 2008;36(1):163-70.

3. Vigo E, Cepeda A, Gualillo O, Perez-Fernandez R. In-vitro antiinflammatory effect of Eucalyptus globulus and Thymus vulgaris: nitric oxide inhibition in J774A.1 murine macrophages. Pharm Pharmacol.2004;56(2):257-63.

4. Akkoç N, Akçelik M, Haznedaroglu IC, Goker H, Turgut M, Aksu S. In vitro anti-bacterial activities of Ankaferd medicinal plant extract. T Klin J Med Sci. 2009;29(2):410-5.

5. Tsai ML, Lin CC, Lin WC, Yang CH. Antimicrobial, antioxidant and anti-inflammatory activities of essential oils from five selected herbs. Biosci Biotechnol Biochem. 2011;75(10):1977-83.

6. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem. 2001;49(11):5165-70.

7. Somogyi A, Rosta K, Pusztai P, Tulassay Z, Nagy G. Antioxidant measurements. Physiol Meas. 2007;28(4):R41-55.

8. Evans MD, Dizdaroğlu M, Cooke MS. Oxidative DNA damage and disease: induction, repair and significance. Mutat Res Rev. 2004;567(1):1-61.

9. Arnhold J. Free radicals-Friends or foes? Properties, functions, and secretion of human myeloperoxidase. Biochemistry (Moscow). 2004;69 (1):4-9.

10. Klebanoff SJ. Myeloperoxidase. Proc Assoc Am Physician. 1999;111(5):383-9.

11. Kotyza J, Buňatová K, Pešek M, Pužman P. Pleural injury and pleurisy-induced progelatinase B/proMMP-9 is associated with markers of neutrophil degranulation. Scand J Clin Lab Invest. 2006;66(6):487-9.

12. Temneanu OR, Zamfir C, Eloaie Zugun F, Cojocaru E, Tocan L: Oxidants and antioxidants relevance in rats’ pulmonary induced oxidative stress. J Med Life. 2011;4(3):244-49.

13. Kasai H, Nishiura S: Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents. Nucl Acids Res. 1984;12(4):2137-45.

14. Yokuş B, Çakır DU. Biomarker of invivo oxidative DNA damage; 8- hydroxy-2-deoxyguanosine. T Klin J Med Sci.2002; 22:535-43.

15. Atmaca E, Paksoy A. Oxidative DNA damage and its chromatographic determination. YYU Vet Fak Derg. 2009;20(2):79- 83.

16. De Martinis, B. S., De Lourdes Pires Bianchi, M. Methodology for urinary 8-hydroxy-2ˈ-deoxyguanosine analysis by HPLC with electrochemical detection. Pharmacol Res. 2002;46 (2):129-31.

17. Dizdaroglu, M. Facts about the artifacts in the measurement of oxidative DNA base damage by gas chromatography-mass spectrometry. Free Radic Res.1998;29(6):551-63.

18. Jaruga P, Dizdaroglu M. Identification and quantification of (50R)- and (50S)-8,50-cyclo-20-deoxyadenosines in human urine as putative biomarkers of oxidatively induced damage to DNA. Biochem Bioph Res Co. 2010;397(1):48-52.

19. Kulaksız G, Sancar A. Nucleotide Excision Repair and Cancer. Turk J Biochem.2007;32(3):104-11.

20. Sancar A, Lindsey-Boltz LA, Kacmaz UK, Linn S. Molecular mechanism of mammalian DNA repair and the damage checkpoints. Annu Rev Biochem. 2004;73:39-85.

21. Sambrook J, Fritsche EF, Maniatis T: Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press,2nd edition, New York, 1989.

22. Lang G, Csekeö A, Stamatis GB, Lampl L, Hagman L, Marta GM et al. Efficacy and safety of topical application of human fibrinogen/thrombin- coated collagen patch (Tachocomb) for treatment of air leakage after standart lobectomy. Eur J Cardiothorac Surg. 2004;25(2):160-6.

23. Macchiarini P, Wain J, Almy S, Dartevelle P: Experimental and clinical evaluation of a new synthetic, absorbable sealent to reduce air leaks in thoracic operations. J Thorac Cardiovasc Surg. 1999;117(4):751-8.

24. Yavuz E, Kalaycı MU, Kesici S, Kesici U, Ozkara A, Yalcın O. Efficacy of Ankaferd Blood Stopper in Bleeding Control in Experimental Partial Splenectomy Model. Kafkas Univ Vet Fak Derg. 2012;18(5):753-8.

25. Gul Satar NY, Cangul IT, Topal A,Oktay A, Inan K,Akgul MB: Effects of Ankaferd Blood Stopper (ABS) and Topical Tripeptide Copper Complex (TCC) on Wound Healing in Rats: An Experimental Study. Kafkas Univ Vet Fak Derg. 2014;20(4):545-51.

26. Öner AF, Dogan M, Kaya A, Sal E, Bektas MS, Yesilmen O, et al. New coagulant agent (ankaferd blood stopper) for open hemorraghes in hemophilia with inhibitor. Clin Appl Thromb Hemost. 2010;16(6):705-7.

27. Bilgili H, Kosar A, Kurt M, Onal IK, Goker H, Captug O et al: Hemostatic Efficacy of Ankaferd Blood Stopper in a Swine Bleeding Model. Med Princ Pract. 2009;18(3):165–9.

28. Cipil HS. In vivo hemostatic effect of the medicinal plant extract Ankaferd blood stopper in rats pretreated with warfarin. Clin Appl Thromb Hemost. 2009;15(3):270-6.

29. Metin B, Altınok T, Menevse E, Esen H. Evaluation of the effects of ankaferd blood stopper on rabbits with paranchyme damage: experimental study. Türk Göğüs Kalp Damar Cerr Derg.2013;21:428- 33. (Article in Turkish)

30. .Williams EA, Quinlan GJ, Goldstraw P, Gothard JWW, Evans TW: Postoperative lung injury and oxidative damage in patients undergoing pulmonary resection. Eur Respir J.1998;11(5):1028-34.

31. .Machlin L, Bendich A: Free radical tissue damage: protective role of antioxidant nutrients. FASEB J.1987;1(6):441-5.

32. Szabó C.The pathophysiological role of peroxynitrite in shock, inflammation, and ischemia- reperfusion injury. Shock 1996;6(2):79- 88,.

33. Willy C, Dahouk S, Starck C, Kaffenberger W, Gerngross H, Plappert UG. DNA damage in human leukocytes after ischemia/reperfusion injury. Free Radical Bio Med.2000;28(1):1-12.