SUAT TEKİN, SÜLEYMAN SANDAL, Cemil ÇOLAK

İnsan Prostat Kanseri Hücre Serilerinde Apelin-13'ün Etkileri

Prostat kanseri erkeklerde görülen en yaygın kanser tiplerinden biridir. Değişik tipteki çoğu kanser türleri için kesin çözüm olabilecek bir tedavi yöntemi tam olarak geliştirilememiştir. Yağ dokudan salgılanan apelin, APJ reseptörüne bağlanarak etkilerini ortaya koymaktadır. Artan yağ doku kitlesi ile obezite gelişmekte ve obeziteye bağlı apelin salgısının da arttığı bilinmektedir. Obezitenin kanser gelişiminde bir risk faktörü olması, hem apelinin hem de reseptörünün testis ve prostat dokusunda yaygın bir şekilde bulunması apelinin prostat kanseri üzerinde önemli etkilerinin olabileceğini akla getirmektedir. Bu çalışmada ilk olarak, apelin-13'ün 0.1, 1 ve 10 nM'lik konsantrasyonları, testosteron hormonunun ise 1, 10, 100 nM'lik konsantrasyonları LNCaP (androjen reseptör pozitif) ve DU-145 (androjen reseptör negatif) insan prostat kanseri hücre serilerine ayrı ayrı uygulandı. Daha sonra testosteron hormonunun 1, 10, 100 nM'lik konsantrasyonları ile 24 saat inkübe edilen prostat kanser hücrelerine apelin-13'ün 10 nM'lik dozu uygulandı ve 24 saat inkübasyona bırakıldı. Apelin13 ve testosteron hormonlarının prostat kanseri hücre canlılığı üzerine etkileri, 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay ile belirlendi. Çalışma sonunda uygulanan apelin-13 ve testosteronun LNCaP hücre canlılığını arttırdığı (p

Effects of Apelin-13 on Human Prostate Cancer Lines

Prostate cancer is one of the most prevalent type of cancer in men. The exact solution for the treatment of different types of cancers has not been fully elucideted. Apelin secreted from adipose tissue reveals its effects by binding to APJ receptor. Obesity develops together with increased adipose tissue, and the increase of obesity related-apelin secretion is known. Obesity is a risk factor in the development of cancer, and considering both apelin and its receptor widely localized in the tissues of testis and prostate and apelin may have significant effects on prostate cancer. In the present study, 0.1, 1 and 10 nM concentrations of apelin-13 and three different testosterone concentrations (1, 10 and 100 nM) were separately aplied to human prostate cancer cells with LNCaP (androgen receptor positive) and DU-145 (androgen receptor negative). Then 1, 10 and 100 nM concentrations of testosterone hormone and 10 nM dose of apelin-13 incubated for 24 h in the prostate cancer cells were administrated and were allowed to incubate for 24 h. Effects of apelin-13 and testosterone on prostate diphenyltetrazolium bromide (MTT) assay. At the end of the experiment, DU-145 did not affected cell viability while the treatment with apelin -13 and testosterone increased the viability of LNCaP cells (p<0.001). The proliferation effect was strongly occured when LNCaP cells were treated with together apelin-13 and testosterone (p<0.001) but, no effect was observed in DU-145 cells. In conclusion; apelin-13 had similar proliferative effects with testosterone on LNCaP cells, but had no effect on DU-145 cells. These results suggest that the effects of apelin-13 are similar to testosterone, and it realises their effects through an androgen receptor-dependent mechanism.

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