Kevser TABAN, Nuraniye ERUYGUR, Osman ÜSTÜN

Biological activity studies on the aqueous methanol extract of Anchusa undulataL. subsp. hybrida (Ten.) Coutinho

The genus Anchusa L. (Boraginaceae) is represented by 15 species in the flora of Turkey. Anchusaspecies are used traditionally as wound healing and diuretic agent. In addition, some Anchusaspecies are used as demulcent, expectorant, analgesic, sedative, diaphoretic and antihypertensive purposes. Carminative, antidiabetic, hemostatic and antirheumatical usage is also recorded.The aim of this study is to determine in vitroantioxidant, anti-AChE, anti-BuChE and α-glucosidase activity of methanol extract (Methanol: water = 80:20, v/v) prepared from roots and aerial parts of Anchusa undulataL. subsp. hybrida (Ten.) Coutinho. Antioxidant activity was evaluated using ABTS and DPPH radical scavenging assay, total phenolic and total flavonoid content. IC50values for DPPH were 239.47 and 292.04 μg/mL and for ABTS were calculated as 41.15 and 32.3 μg/ml. The total phenolic content of root and herbal extract were found as 130.39 and 122.94 mg/g, while flavonoid content as 65.25 and 117.5 mg/g. In the enzyme inhibition studies, the methanol extract showed cholinesterase and α-glucosidase enzyme inhibition activities with the increasing concentrations. The significant enzyme inhibition activities are depended on higher amount of phenolic compounds.

PDF

___

[1]Koz O, Pizza C, Kırmızıgül S. Triterpene and flavone glycosides from Anchusa undulatasubsp. hybrida.Nat Prod Res. 2009; 23(3): 284-292.

[2]Kuruuzum-Uz A, Suleyman H, Cadirci E, Guvenalp Z, Demirezer LO. Investigation on anti-inflammatory and antiulcer activities of Anchusa azureaextracts and their major constituent rosmarinic acid. Z Naturforsch C. 2012; 67(7-8): 360-366.

[3]Kuruuzum-Uz A, Guvenalp Z, Kazaz C, Salih B, Demirezer LO. Four new triterpenes from Anchusa azureavar. azurea. Helv Chim Acta. 2010; 93(3): 457-465.

[4]Deveci E, Tel-Çayan G, Yıldırım H, Duru ME,. Chemical composition, antioxidant, anticholinesterase and anti-urease activities of Sideritis pisidicaBoiss. & Heldr. endemic to Turkey. Marmara Pharm J. 2017; 21(4): 898-905.

[5]Telagari M, Hullatti K. In-vitroα-amylase and α-glucosidase inhibitory activity of Adiantum caudatumLinn. and Celosia argenteaLinn. extracts and fractions. Ind J Pharmacol. 2015; 47(4): 425-429.

[6]Abirami A, Nagarani G, Siddhuraju P. In vitroantioxidant, anti-diabetic, cholinesterase and tyrosinase inhibitory potential of fresh juice from Citrus hystrixand C.maximafruits. Food Sci Hum Wellness. 2014; 3(1): 16-25.

[7]Kumarappan C, Mandal SC. α-Glucosidase inhibitory activity and in vitro antioxidant activities of alcohol-water extract (AWE) of Ichnocarpus frutescensleaves. Med Chem Res.2008; 17(2-7): 219-233.

[8]Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc.2007; 2(4): 875-876.

[9]Yang H, Dong Y, Du H, Shi H, Peng Y, Li X. Antioxidant compounds from propolis collected in Anhui, China. Molecules. 2011; 16(4): 3444-3455.

[10]Wang H, Gao XD, Zhou GC, Cai L, Yao WB. In vitroand in vivoantioxidant activity of aqueous extract from Choerospondias axillarisfruit. Food Chem.2008; 106(3): 888-895.

[11]Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free RadicBiol Med. 1999; 26(9-10): 1231-1237.

[12]Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol.1961; 7(2): 88-95.

[13]Ademiluyi AO, Oboh G. Soybean phenolic-rich extracts inhibit key-enzymes linked to type 2 diabetes (α-amylase andα-glucosidase) and hypertension (angiotensin I converting enzyme) in vitro. Exp Toxicol Pathol. 2013; 65(3): 305-309.