Beste M. ATASOY, Güngör Mine GÜLLÜOĞLU, Ufuk ABACIOĞLU, Mustafa DENİZ, Meriç SENGÖZ, Berrak YEĞEN

Granulocyte colony stimulating factor ameliorates radiation-induced morphological destruction of intestinal mucosa in rats

Amaç: Profilaktik olarak uygulanan G-CSF’nin, radyasyona bağlı akut dönemde intestinal mukozada gelişen hasarı azaltmadaki rolü araştırıldı. Yöntem: Wistar-Albino türü dişi sıçanlar fizyolojik tuzlu su (FTS) öntedavisi veya G-CSF öntedavisi verilen kontrol grupları ile FTS tedavisi verilmesinin ardından radyasyon uygulanan ve radyasyon öncesi G-CSF uygulanan gruplar olmak üzere 4 gruba ayrıldı. G-CSF öntedavisi alan iki grupta sıçanlara G-CSF (100µg/kg/gün) 4 gün boyunca deri altından uygulandı. Son ilaç uygulamasından 12 saat sonra sıçanların yarısına, anestezi altında pelvik alana tek doz 14 Gy radyasyon verildi. FTS veya G-CSF verilen kontrol gruplarındaki sıçanlara ise radyasyon uygulanmadı. Bütün sıçanlar ışınlamadan sonraki 4. günün başında dekapite edildi, terminal ileum ve rektumdan alınan örnekler mikroskopik olarak değerlendirildi. Bulgular: Terminal ileum örnekleri incelendiğinde, G-CSF uygulanan kontrol grubu ile FTS uygulanmış kontrol grupları arasında anlamlı bir fark yoktu. Işınlanan gruplardan FTS verilen radyasyon grubundaki skor tüm gruplara gore anlamlı olarak daha yüksek bulundu. Buna karşılık, G-CSF uygulanan grupta bu skorun anlamlı olarak düşük olduğu gözlendi. G-CSF uygulanan ve FTS uygulanan radyasyon gruplarının rektum örnekleri arasında ise mikroskopik hasar açısından anlamlı bir değişiklik oluşmadı. Sonuç: Deneysel çalışmamız, iyonizan radyasyon öncesi G-CSF uygulanmasının terminal ileum mukozasının morfolojik yapısının korunmasında etkili olduğunu, ancak aynı etkinin rektumda oluşmadığını göstermektedir.

Granülosit koloni stimülan faktörün sıçanlarda radyasyona bağlı gelişen ince barsak mukoza hasarını azaltıcı etkisi

Objective: We aimed to evaluate whether prophylactic administration of granulocyte colony-stimulating factor (G-CSF) has a protective effect on the morphology of irradiated intestinal mucosa. Methods: Twenty-three animals were divided into 4 groups: saline-treated control and G-CSF-injected control groups, irradiated group with saline injection and irradiated and G-CSF administered group. G-CSF (100µg/kg/day) was given subcutaneously for four consecutive days. Twelve hours after the fourth injection, under anaesthesia a single pelvic dose of 14 Gy ionising radiation was given to irradiated groups, while the control groups were not irradiated. On the fourth day of irradiation or sham-radiation, rats were sacrificed and terminal ileum and rectum samples were removed for histological assessment of mucosal injury. Results: Terminal ileum samples from both G-CSF treated and saline-treated control groups revealed minimum scores. In the saline-treated IR group, the score was significantly elevated as compared to either control groups. However, G-CSF administration preceding the irradiation reduced the severity of mucosal damage. On the other hand, the increased scores in the rectum samples of irradiated rats treated with either saline or G-CSF were not different from each other. Conclusion: Prophylactic administration of G-CSF might reduce ionising radiation-induced mucosal destruction of the terminal ileum, but not the rectum.

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