Emel ÖZVEREN, Feyza İNCİ, Rüya GÖZ, F. Yılmaz GÖĞÜŞ, Mustafa DENİZ

In vitro effects of intravenous propofol infusion on cytokine gene expression in rats

Amaç: Bu çalışmanın amacı; sıçanlarda intravenöz propofol infüzyonunun sitokin gen ekspresyonuna etkisinin invitro araştırılmasıdır. Metod: 300-320 g ağırlığında, 20 adet, erkek, Spraque-Dawley cinsi sıçan iki gruba ayrıldı (n:10). Her iki gruba orta hat laparotomi insizyonu uygulandıktan sonra 90 dakika süre ile Grup I'e 5ml/kg/s serum fizyolojik infüzyonu, Grup II'ye 5ml/kg/s propofol infüzyonu verildi. İnfüzyon bitiminde mezenter lenf nodu kemik iliği aspiratları IL-1, IL-6 and TNFα ölçümleri için alındı. Ölçümler; real time polimeraz zincir reaksiyonu (RT-PCR) yöntemi ile yapıldı. Bulgular: Mezenter lenf nodlarında IL-1 (p

Sıçanlarda intravenöz propofol infüzyonunun sitokin gen ekspresyonuna etkisinin invitro araştırılması

Objective: The aim of this study was to evaluate the effects of propofol infusion on cytokine gene expression levels of bone marrow and mesentheric lymph nodes in rats after surgical trauma. Methods: Twenty male Sprague-Dawley rats weighing between 300-320 g were divided into two groups (n=10). Midline laparatomy incision was performed to both groups. Group I received 5 ml/kg/hr iv serum physiologic infusion. Group II received 5 mg/kg/hr iv propofol infusion. After infusions, mesentheric lymph node and bone marrow samples were obtained for the measurement of IL-1, IL-6 and TNFα gene expression levels by real time polymerase chain reaction (RT-PCR) method. Results: IL-1 (p<0.002) and TNFα (p<0.018) levels of the mesentheric lymph node were significantly lower in group II compared with group I. TNFα gene expression level in the bone marrow was also significantly lower in group II compared with group I (p<0.0015). Conclusion: Propofol infusion during surgery caused significantly more proinflammatory cytokine gene expression supression in mesentheric lymph nodes compared to bone marrow in rats. We concluded that propofol infusion may have a limited and especially local immune supressive effect.

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