The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification

Fusarium türleri bağışıklıkları baskılanmış hastalar için önemli fırsatçı patojenlerdir. Bu çalışmada, diyabetik ayaklı bir hastadan izole edilen patojenin, konvansiyonel ve moleküler tekniklerle tanımlanması yapılarak, Fusarium sporotrichioides olduğu sonucuna varıldı. HPLC analizleri, patojenin T-2 toksinini üretmediği görüldü. Dolayısıyla, laboratuvar tanılarında, Fusarium türleri basit çevresel kontaminantlar olarak düşünülmemelidir.

Diyabetik ayaklı bir hastaya ait yara örneğinden fusarium sporotrichioides izolasyonu ve tanımlanması

Fusaria are major opportunist pathogens for immunocompromised patients. In this study, it was concluded as a result of both conventional and molecular identification techniques that the isolate obtained from the patient’s diabetic foot was Fusarium sporotrichioides. T-2 toxin production of the pathogen was investigated using HPLC and no toxin determined. In laboratory diagnosis, it must not be thought that Fusaria are solely an environmental contaminant.

___

  • 1) Brown DW, McCormick SP, Alexander NJ, Proctor RH, Desjardins AE. Inactivation of a cytochrome P-450 is a determinant of trichotecene diversity in Fusarium species. Fungal Genet Biol 2002; 36: 224-233.
  • 2) Kenedy MJ, Sigler L. Aspergillus, Fusarium, and other opportunistic moniliaceous fungi. In: Murray P, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds., Manuel of Clinical Microbiology, 6th ed. Washington D C: ASM Press, 1995; 765-790.
  • 3) Cenis JL. Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Res 1992; 20: 2380.
  • 4) Wilson A, Simpson D, Chandler E, Jennings P, Nicholson P. Development of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae. FEMS Microbiol Lett 2004; 233: 69-76.
  • 5) Jimenez M, Mateo JJ, Mateo R. Determination of typeA trichothecenes by determination of typeA trichothecenes by high-performance liquid chromatography with coumarin-3-carbonyl chloride derivatisation and fluorescence detection. J Chromatogr 2000; A 870: 473-481.
  • 6) Jimenez M, Mateo R. Determination of mycotoxins produced by Fusarium isolates from banana fruits by capillary gas chromatography and high-performance liquid chromatography. J Chromatogr 1997; 778: 363-372.
  • 7) White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfland DH, Sninsky JJ, White TJ. (eds.), PCR Protocols, San Diego, Academic Press, 1990; 315-322.
  • 8) Waalwijk C, De Koning JRA, Baayen RP, Gams W. Discordant groupings of Fusarium spp. from the sections Elegans, Liseola and Dlaminia based on ribosomal ITS1 and ITS2 sequences. Mycologia 1996; 88; 361-368.
  • 9) Samson RA, Hoekstra ES Oorschot CA. Introduction to food-borne fungi. 2nd ed. CBS, The Nederland. 1984.
  • 10) Mateo JJ, Mateo R, Jimenez M. Accumulation of type A trichothecenes in maize, wheat and rice by F. sporotrichioides isolates under diverse culture conditions. Int J Food Microbiol 2002; 72: 115-123.
  • 11) Bigley VH, Duarte RF, Gosling RD, Kibbler CC, Seaton S, Potter M. Fusarium dimerum infection in a stem cell transplant recipient treated successfully with voriconazole. Bone Marrow Transpl 2004; 34: 815-817.
  • 12) Moschovi M, Trimis G, Anastasopoulos J, Kanariou M, Raftopoulou A, Tzortzatou-Stathopoulou F. Subacute vertebral osteomyelitis in a child with diabetes mellitus associated with Fusarium. Pediatr Int 2004; 46: 740-742.
  • 13) Hemashettar BM, Siddaramappa B, Padhye AA, Sigler L, Chandler FW. Wite grain mycetoma caused by a cylindrocarpon sp. in India. J Clin Microbiol 2000; 38: 4288-4291.
  • 14) Cocuroccia B, Gaido J, Gubinelle E, Annessi G, Girolomoni G. Localized cutaneous hyalohyphomycosis caused by a Fusarium species infection in a renal transplant patient. J Clin Microbiol 2003; 41: 905-907.
Marmara Medical Journal-Cover
  • ISSN: 1019-1941
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 1988
  • Yayıncı: Marmara Üniversitesi
Sayıdaki Diğer Makaleler

Brucella epididymo-orchitis as the first presenting sign of brucellosıs: A case report and review of the literature

Yılören TANIDIR, Abdülkadir GÜMRAH, Cem AKBAL, Tufan TARCAN

The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification

MUSTAFA ÖZYURT, Nurittin ARDIÇ, KADİR TURAN, ŞENOL YILDIZ, Oğuz ÖZYARAL, Uğur DEMİRPEK, Tunçer HAZNEDAROĞLU, Türkan YURDUN

İNFERTİLİTEDE TANISAL LAPAROSKOPİ MUTLAK GEREKLİ MİDİR?

Recep YILDIZHAN, Necdet SÜER

ACİL SERVİSE BAŞVURAN ZEHİRLENME OLGULARININ GERİYE DÖNÜK ANALİZİ

Onur YEŞİL, Haldun AKOĞLU, Özge ONUR, Özlem GÜNEYSEL

DİYABETİK AYAKLI BİR HASTAYA AİT YARA ÖRNEĞİNDEN FUSARIUM SPOROTRICHIOIDES İZOLASYONU VE TANIMLANMASI

Mustafa ÖZYURT, Nurittin ARDIÇ, Kadir TURAN, Şenol YILDIZ, Oğuz ÖZYARAL, Uğur DEMİRPEK, Tuncer HAZNEDAROĞLU, Türkan YURDUN

İnfertilitede tanısal laparoskopi mutlak gerekli midir?

Recep YILDIZHAN, Necdet SÜER

SIÇANLARDA İNTRAVENÖZ PROPOFOL İNFÜZYONUNUN SİTOKİN GEN EKSPRESYONUNA ETKİSİNİN İNVİTRO ARAŞTIRILMASI

Emel ÖZVEREN, Feyza İNCİ, Rüya GÖZ, F. Yılmaz GÖĞÜŞ, Mustafa DENİZ

PARADOKSİKAL MİYOKARD İNFARKTÜSU TEKRAR ZİYARETTE

Atilla Ener RASIH, Behnam BOZORGNIA, Babak BOZORGNIA

İNTRASKROTAL EKSTRATESTİKÜLER NÖROFİBROMA: OLGU SUNUMU

Fikret ERDEMİR, Bekir Süha PARLAKTAŞ, Nihat ULUOCAK, Nurpek Onuk FİLİZ, Berat ACU, REŞİT DOĞAN KÖSEOĞLU, Başar SARIKAYA

İSTANBUL’DA BİR TIP FAKÜLTESİ HASTANESİNDE YATAN HASTALARIN MEMNUNİYET DÜZEYİ

Muhammed ÖNSÜZ, Ahmet TOPUZOĞLU, Utku CÖBEK, Sercan ERTÜRK, Fatma YILMAZ, Selim BİROL