Nurhan KESKİN, Fethi Ahmet ÖZDEMİR, Mustafa ÇELİK

‘Karaerik’ Üzüm Çeşidinde in vitro Fenolik Bileşik Üretimi Üzerine UV Işını Uygulaması ve İnkübasyon Sürelerinin Etkisi

Bu çalışmada, Karaerik üzüm çeşidine ait kallus kültürlerinde, bireysel ve toplam fenolik bileşik üretiminin uyarılması üzerine Ultraviyole (UV) ışınının etkisi incelenmiştir. Kallus kültürleri, in vitro bitkiciklerin yaprak ayalarından elde edilmiştir. Kültür ortamı olarak, 0.1 mg l -1 NAA (Naftalen asetik asit) ve 0.2 mg l-1 Kin (Kinetin) eklenmiş Gamborg B-5 ortamı kullanılmıştır. Kalluslar, 21 gün ara ile iki defa alt kültüre alınmıştır. İkinci alt kültürden sonra, 12 gün yaşlı kalluslara, steril kabin içerisinde petri kutularının kapakları açılarak, 10 cm uzaklıktan 10 ve 15 dk süreyle 254 nm dalga boyuna sahip UV-C ışını uygulanmıştır. Uygulamanın ardından kalluslar karanlık koşullarda inkübe edilmiştir. Fenolik bileşik ölçümleri 24., 48. ve 72. saatlerde yapılmıştır. Bireysel fenolik bileşikler HPLC (Yüksek Basınçlı Sıvı Kromatografisi), toplam fenolik bileşikler ise Spektrofotometre ile analiz edilmiştir. Çalışma sonucunda UV ışınının Karaerik üzüm çeşidine ait kallus dokularında fenolik bileşik üretimini uyarmada etkili ve bu etkinin uygulama süresi ile yakından ilişkili olduğu saptanmıştır.

The Effects of UV Irradiation and Incubation Time on in vitro Phenolic Compound Production in 'Karaerik' Grape Cultivar

In this study, the effect of Ultraviolet (UV) irradiation on induction ofindividual and total phenolics production on callus cultures of‘Karaerik’ grape cultivar was investigated. Callus tissues wereobtained from the leaves of the cuttings grown in in vitro plants. As aculture medium, Gamborg B-5 was utilized with 0.1 mg L-1 NAA(Naphthaleneacetic acid) and 0.2 mg L-1 Kin (Kinetin). Callus tissueswere sub-cultured twice with 21 days intervals. After the secondsubculture, 12-day-old callus tissues were exposed to 254 nm UV-Clight at 10 cm distance from the source for 10 and 15 min by openingcovers of the petri dishes in sterile cabin. After the treatment, callustissues were incubated under dark conditions. Phenolic compoundswere measured at 24th, 48th and 72nd hours. Individual phenoliccompounds were analyzed by HPLC (High Pressure LiquidChromatography) and total phenolic compounds were measured byspectrophotometer. As a result of the study, it was found that UVirradiation was effective for induction the production of phenoliccompounds in the callus tissues of ‘Karaerik’ grape cultivar and thiseffect was closely related to the application time.

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