Simental Irkı Sığırlarda GH ve PIT-I Gen Polimorfizmleri ile Süt Verimleri Arasındaki İlişkinin Araştırılması

Bu çalışmada Simental ırkı sığırlarda büyüme hormonu (GH) vehipofiz spesifik transkripsiyon faktör-1 (PIT-1) genleri ile 1. ve 2.laktasyon toplam süt verimleri ve günlük süt verimleri arasındakiilişkinin araştırılması amaçlanmıştır. Çalışmada, Kayseri ilicivarında yetiştirilen 203 baş Simental ırkı sağmal inekincelenmiştir. GH genotiplerinin belirlenmesi amacıyla yapılan PCRişlemi sonunda elde edilen 223 bp’lik PCR ürünleri AluI restriksiyonenzimiyle kesilmiştir. PIT-1 genotiplerinin belirlenmesi amacıylayapılan PCR işlemi sonunda elde edilen 600 bp’lik PCR ürünleri iseHinfI enzimi ile kesilmiştir. GH-AluI ve PIT-1-HinfI genotipleri ilegünlük ve laktasyon toplam süt verimleri arasındaki ilişki tek yönlüvaryans analizi (ANOVA) ile değerlendirilmiştir. GH-AluIpolimorfizmi yönünden incelenen Simental ırkı sığırlarda iki allel (Ave B) ile üç genotip (LL, LV ve VV) gözlenmiştir. İncelenenörneklerde LL genotip frekasının en yüksek (0.52), VV genotipfrekansının ise en düşük (0.11) olduğu belirlenmiştir. PIT-1-HinfIpolimorfizmi yönünden incelenen Simental ırkı ineklerde iki genotip(AB ve BB) gözlenmiştir. AA genotipine incelenen örneklerderastlanılmamıştır. İncelenen Simental ırkı sığırlarda BB genotipfrekasının en yüksek (0.74) olduğu belirlenmiştir. Çalışma sonundaSimental ırkı ineklerde GH-AluI ve PIT-1-HinfI polimorfizmleri ile1., 2. laktasyon toplam süt verimleri ve günlük süt verimi arasındaistatistik olarak önemli ilişki olmadığı belirlenmiştir (P>0.05).Simental ırkı sığırlarda GH-AluI ve PIT-1-HinfI polimorfizmleri ilediğer süt verim özellikleri ile büyüme ve et verim özellikleriarasındaki ilişkilerin araştırıldığı çalışmaların planlanmasıgerektiği düşünülmüştür.

Investigation of the Relationship between GH and PIT-I Gene Polymorphisms and Milk Yields in Simmental Cattle Breed

The aim of this study was to investigate the relationship between growth hormone (GH) and pituitary-specific transcription factor-1 (PIT-1) genes and 1th, 2nd lactation total milk yield and daily milk yield in Simental cattle breed. In this study 203 Simmental dairy cows, reared in Kayseri vicinity, were examined. In order to determine GH genotypes, 223 bp PCR products obtained by PCR were digested with AluI restriction enzyme. In order to determine PIT-1 genotypes, 600 bp PCR products obtained by PCR were digested with HinfI restriction enzyme. The mean differences of 305- day milk yield among the genotype groups of GH-AluI and PIT-1- HinfI were assessed by one-way analysis of variance (ANOVA). Two alleles (A and B) and three genotypes (AA, AB and BB) for GH-AluI polymorphism were observed in examined Simmental cows. The LL genotype frequency was found highest (0.52) and the VV genotype frequency was found the lowest (0.11) in examined Simmental cows. The frequency of the L allele was found higher (0.7) than V allele (0.3) in this study. Two alleles (A and B) and two genotypes (AB and BB) for PIT-1-HinfI polymorphism were observed in examined Simmental cows in this study. The AA genotype was not found in the examined samples. The BB genotype frequency was found to be highest (0.74) in examined Simnetal cows. End of the study, the genotype effects for GH-AluI and PIT-1-HinfI polymorphisms on daily and lactation total milk yield were not found to be significant in examined Simmental cows (P>0.05). It was thought that further studies on the association between GH-AluI and PIT-1-HinfI polymorphism and other milk yield traits and meat yield traits in Simmental cattle breed should be conducted.

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