Farklı Mikroalg ve Ticari Yemlerin Rotifer (Brachionus plicatilis , Müller 1786) Büyümesi, Protein ve Yağ Asidi Profiline Etkisi
Rotifer (Brachionus plicatilis) deniz balıkları yetiştiriciliğinde larvaldönemde kullanılan ilk canlı yemdir. Bu dönemde besince zengin vekaliteli rotifer kullanımı larvaların hayatta kalma oranlarınınyüksek olmasında rol oynamaktadır. Rotiferler esansiyel yağasitleri, amino asitler, vitamin ve mineraller bakımından denizbalığı larvalarının doğal besinleri olan kopepodlara kıyasla besincedaha eksiktir. Bu sebeple, rotifer kültüründe kısa sürede rotifersayısının çoğaltılması ve besin değeri yüksek rotiferler elde edilmesiönem göstermektedir. Bu çalışmadaki amaç, taze olarak kültüredilen mikroalgler ( Chlorella vulgaris ve Dunaliella salina ) ve ticarirotifer yemleri (Ekmek mayası ® , S.parkle ® ve Ekmek mayası+W-3 ® )ile beslenen rotiferlerin büyüme performansı, besin maddeleri veyağ asidi kompozisyonunlarının incelenmesidir. Chlorella vulgarisve Dunaliella salina sırasıyla 3N-BBM+V ve f/2 besi yerlerindekültür edilmiş ve 7 gün süresince 5 deney yemi ile rotiferlerbeslenmiştir. Deney sonunda, ‘’Ekmek mayası+W-3’’ karışımı ilebeslenen rotifer grubu en yüksek rotifer yoğunluğuna ulaşmıştır.Chlorella vulgaris ile beslenen rotiferler yüksek oranda oleik asitiçermiştir. Ekmek mayası ile beslenen rotifer grubunda ise EPAyüksek oranda bulunmuştur (P
Effect of Different Microalgae and Commercial Feeds on Growth, Protein and Fatty Acid Profile of Rotifer ( Brachionus plicatilis)
Rotifer is the first live prey for marine fish larvae. At that stage, the quality of live prey such as rotifer (Brachionus plicatilis), which is the first feed of larvae, play an important role in survival. Rotifers are known to lack essential fatty acids (EFA), essential amino acids (EAA), vitamins and minerals in comparison to the copepods, the natural feed of marine fish larvae. Therefore, before the enrichment process, increasing growth rate of rotifer in short time with high nutritional value is very important. In this study, effects of two freshly cultured microalgae ( Chlorella vulgaris and Dunaliella salina ) and three commercial rotifer feeds (Beaker’s yeast ® , S.parkle ® and Beaker’s yeast+W-3 ® ) were evaluated for growth performance, proximate composition and fatty acid profile of rotifers. Chlorella vulgaris ve Dunaliella salina were cultivated in 3N-BBM+V and f/2 medium respectively. Rotifers were fed with five experimental diets during 7 days of feeding experiment. At the end of the experiment, rotifers fed ‘’Beaker’s yeast+W3’’ showed highest rotifer population among experimental groups. Oleic acid level was found higher in rotifer fed Chlorella vulgaris group and another important fatty acid EPA was found higher in rotifer fed Beaker’s yeast. In conclusion, that commercial products have high potential on the numbers of produced rotifers, however, microalgae are still important for obtaining high essential fatty acid profile in terms of delivering essential nutrients.
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- AOAC 1998a. Official method 980.46, Moisture in
meat. Meat and meat products (Official Methods of
Analysis of AOAC International: Ed. Soderberg,
D.L. Gaitherbury, Maryland, USA).
- AOAC 1998b. Official method 955.04, Nitrogen (total)
in seafood. Fish and other marine products (Official
methods of analysis of AOAC International: Ed:
James M. Hungerford and P. Cunniff).
- Arndt C, Sommer U 2014. Effect of algal species and
concentration on development and fatty acid
composition of two harpacticoid copepods, Tisbe sp.
and Tachidius discipes , and a discussion about their
suitability for marine fish larvae. Aquaculture
Nutrition, 20(1): 44-59.
- Borowitzka MA, 2013. High-value products from
microalgae
their
development
and
commercialisation. Journal
of
Applied
Phycology, 25(3): 743-756.
- Brown MR, Jeffrey SW, Volkman JK, Dunstan GA
1997. Nutritional properties of microalgae for
mariculture. Aquaculture, 151(1): 315-331.
- Christie WW 1982. Lipid Analysis. Pergamon Press,
Oxford, UK.
- Dhert P, Rombaut G, Suantika G, Sorgeloos P 2001.
Advancement of rotifer culture and manipulation
techniques in Europe. Aquaculture, 200(1): 129-
146.
- Dhert P, King N, O'Brien E 2014. Stand-alone live food
diets, an alternative to culture and enrichment
diets for rotifers. Aquaculture, 431: 59-64.
- Drillet G, Frouël S, Sichlau MH, Jepsen PM, Højgaard
JK, Joarder AK, Hansen BW 2011. Status and
recommendations on marine copepod cultivation for
use as live feed. Aquaculture, 315(3): 155-166.
- Ferreira M, Maseda A, Fábregas J, Otero A 2008.
Enriching rotifers with “premium” microalgae.
Isochrysis
aff.
galbana
clone
T-
ISO. Aquaculture, 279(1), 126-130.
- Folch JL, Lees M, Stanley GHS 1957. A simple methodfor the isolation and purification of total lipids from
animal tissues. The Journal of Biological
Chemistry, 226:497-509.
- Ganuza E, Benitez-Santana T, Atalah E, Vega-
Orellana O, Ganga R, Izquierdo M 2008.
Crypthecodinium cohnii and Schizochytrium sp. as
potential substitutes to fisheries-derived oils from
seabream (Sparus aurata) microdiets. Aquaculture,
277:109-116.
- Hayashi M, Yukino T, Watanabe F, Miyamoto E,
Nakano Y 2007. Effect of vitamin B12-enriched
thraustochytrids on the population growth of
rotifers. Bioscience,
biotechnology,
and
biochemistry, 71(1): 222-225.
- Hemaiswarya S, Raja R, Kumar RR, Ganesan V,
Anbazhagan C 2011. Microalgae: a sustainable feed
source
for
aquaculture. World
Journal
of
Microbiology and Biotechnology, 27(8): 1737-1746.
- Hirayama K, Maruyama I, Maeda T 1989. Nutritional
effect of freshwater Chlorella on growth of the
rotifer Brachionus plicatilis . Hydrobiologia, 186(1):
39-42.
- IUPAC 1987. Standart Methods for The Analysis of
Oils, Fats and Derivatives. 6th Edition (Fifth
Edition Method II.D.19), Pergamon Press, Oxford,
96-102.
- Izquierdo MS, Watanabe T, Takeuchi T, Arakawa T,
Kitajima C 1990. Optimal EFA levels in Artemia to
meet the EFA requirements of red seabream
( Pagrus major ) (The Current Status of Fish
Nutrition in Aquaculture: Ed. by Takeda M,
Watanabe T) 221–232.
- Izquierdo MS 2005. Essential fatty acid requirements
in Mediterranean fish species. Cah. Options
Mediterr, 63: 91-102.
- Lee YK 2001. Microalgal mass culture systems and
methods: their limitation and potential. Journal of
Applied Phycology, 13(4): 307-315.
- Lee BI, Kim DJ, Kim SK, Lee NS, Hagiwara A, Kwon
ON, Park HG, Park JC 2016. Optimal Food and
Concentration for Growth of Small Rotifer , Proales
similis . Journal of Fisheries and Marine Sciences
Education, 28(2): 315-322.
- Kennari AA, Ahmadifard N, Seyfabadi J, Kapourchali
MF 2008. Comparison of growth and fatty acids
composition of freshwater rotifer, Brachionus
calyciflorus Pallas, fed with two types of microalgae
at different concentrations. Journal of the World
Aquaculture Society, 39(2): 235-242.
- Kim HJ, Nakamura K, Hagiwara A 2014. Dietary
effect of selenium‐fortified Chlorella vulgaris on
reproduction of Brachionus plicatilis species complex (Rotifera: Monogononta). International
Review of Hydrobiology, 99(1-2): 161-165.
- Kobayashi T, Nagase T, Hino A, Takeuchi T 2008.
Effect of combination feeding of Nannochloropsis
and freshwater Chlorella on the fatty acid
composition of rotifer Brachionus plicatilis in a
continuous culture. Fisheries science, 74(3): 649-
656.
- Li K, Olsen Y 2015. Effect of enrichment time and
dietary DHA and non-highly unsaturated fatty acid
composition on the efficiency of DHA enrichment in
phospholipid
of
rotifer
(Brachionus
Cayman). Aquaculture, 446: 310-317.
- Maruyama I, Nakao T, Shigeno I, Ando Y, Hirayama
K 1997. Application of unicellular algae Chlorella
vulgaris for the mass-culture of marine rotifer
Brachionus. Hydrobiologia, 358(1-3): 133-138.
- Maruyama I, Yamamoto S, Hayashi M, Murata O
2006. Rotifers fed with n-3 highly unsaturated fatty
acid-enriched Chlorella vulgaris are suitable for the
rearing of larval red sea bream Pagrus
major . Aquaculture Science, 54(2): 229-230.
- Nanton DA, Castell JD 1999. The effects of
temperature and dietary fatty acids on the fatty
acid composition of harpacticoid copepods, for use
as a live food for marine fish larvae. Aquaculture
175: 167–181.
- Nhu CV 2004. A Comparison of yield and quality of the
rotifer ( Brachionus plicatilis -L. Strain) fed different
diets under aquaculture conditions, Vietnam. Asian
Fisheries Science, 17: 357-363.
- Nordgreen A, Penglase S, Hamre K 2013. Increasing
the levels of the essential trace elements Se, Zn, Cu
and Mn in rotifers ( Brachionus plicatilis ) used as
live feed. Aquaculture, 380 :120-129.
- Olsen Y, Van der Meeren T, Reitan KI 2004. First
Feeding Technology (Moksness E, Kjørsvik E, Olsen
Y) 279-333.
- Qie G, Reitan KI, Olsen Y 1994. Comparison of rotifer
culture quality with yeast plus oil and algal-based cultivation diets. Aquaculture International, 2(4):
225-238.
- Penglase S, Hamre K, Sweetman JW, Nordgreen A
2011. A new method to increase and maintain the
concentration of selenium in rotifers (Brachionus
spp.). Aquaculture, 315(1): 144-153.
- Pulz O, Gross W 2004. Valuable products from
biotechnology of microalgae. Applied microbiology
and biotechnology, 65(6): 635-648.
- Ribeiro ARA, Ribeiro L, Dinis MT, Moren M 2011.
Protocol to enrich rotifers (Brachionus plicatilis)
with
iodine
and
selenium. Aquaculture
Research, 42(11): 1737-1740.
- Reitan KI, Rainuzzo JR, Øie G, Olsen Y 1993.
Nutritional effects of algal addition in first-feeding
of
turbot
( Scophthalmus
maximus
L.)
larvae. Aquaculture, 118(3): 257-275.
- Sargent JR, McEvoy LA, Bell JG 1997. Requirements,
presentation and sources of
- Polyunsaturated fatty acids in marine fish larval feeds.
Aquaculture 155: 117–127.
- Spolaore P, Joannis-Cassan C, Duran E, Isambert A
2006.
Commercial
applications
of
microalgae. Journal
of
Bioscience
and
Bioengineering, 101(2): 87-96.
- Sayın S, Ișık O, Polat S 2000. The feeding of the rotifer
Brachionus plicatilis Müller, 1786 with different
microalgae species, Isochrysis galbana Parke,
Tetraselmis chuii (Bucker), Rhinomonas reticulata
(Lucas) Novamizo, Pavlova lutheri (Droop) Green
and Chlorella vulgaris (Beijerinck). Turkish
Journal of Biology, 24: 87-95.
- Zaki MI, Saad H 2010. Comparative study on growth
and survival of larval and juvenile Dicentrarchus
labrax rearing on rotifer and Artemia enriched with
four different microalgae species. African Journal of
Biotechnology, 9(24): 3676-3688.