Bazı Schiff Bazlarının Saccharomyces cerevisiae BY4741 Kültür Ortamlarında Biyokimyasal Parametreler Üzerine Etkileri
Yeni sentezlenmiş bazı Schiff bazlarının kullanılmadan öncecanlılar üzerindeki biyokimyasal etkilerinin incelenmesi gerekir.Bu amaçla Saccharomyces cerevisiae en önemli hücre modeli içindeyer alır. S. cerevisiae’daki metabolik özellikler, yüksek yapılıorganizmalara benzerlik gösterdiği için elde edilen sonuçlar daparalellik göstermektedir.Çalışmada; yeni sentezlenmiş Schiff bazlarının S. cerevisiaeBY4741 kültür ortamlarında lipit peroksidasyon (MDA), totalprotein ve glutatyon analizleri yapılmıştır. Bu amaçla; deneydekullanılan S. cerevisiae BY4741’in gelişimi ve çoğalması için YEDPbesiyeri ortamı hazırlandı. Uygulama grupları için; schiff bazlarınher birinden 2ppm, 4 ppm ve 8 ppm olacak şekilde kültür ortamınailave edildi. Elde edilen süpernatant ile; GSH,total protein ve MDAanalizleri yapıldı.Lipid peroksidayon sonuçları kontrol grubu ile karşılaştırıldığındaschiff bazı gruplarının lipid peroksidasyon önleme etkilerininolmadığı belirlenirken, yeni sentezlenen schiff bazlarının kontrolgrubuna göre glutatyon ve total protein miktarlarının oldukçabelirgin düzeyde yüksek olduğu saptandı (p
The Effects of Some Schiff Bases on Biochemical Parameters in Saccharomyces cerevisiae BY4741 Cultural Environments
Biochemical effects of newly synthesized some Schiff bases on living creatures need to be investigated before their use. For this purpose, Saccharomyces cerevisiae is involved in the most important cell model. Because metabolic properties of S. cerevisiae are similar to the highly organized organisms, the results show parallelism, as well. In the present study; lipid peroxidation (MDA), total protein, and glutathione analyses of newly synthesized Schiff bases were analysed in S. cerevisiae BY4741 culture media. For this purpose, YEDP medium was prepared for development and growth of S. cerevisiae BY4741 used in the experiment. In the study, 2 ppm, 4 ppm, and 8 ppm from each of Schiff bases were added in to culture medium for application groups. GSH, total protein and MDA analyses were carried out with the obtained supernatant. As the results of lipid peroxidation were compared to the control group, it was concluded that the Schiff base groups had no lipid peroxidation inhibition effects, and the newly synthesized schiff bases were found to have significantly greater amounts of glutathione and total protein than the control group (p
___
- Ahmed HJ, Ezer N 2008. Prunella L. türlerinin
kimyasal bileşikleri ve biyolojik aktiviteleri.
Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi,
28: 93-113.
- Akkuş İ 1995. Serbest radikaller ve fizyopatolojik
etkileri. 1. Baskı, Mimoza Yayınları, Konya, 3-10s.
- Amirkhanov VM, Bundya EA, Trush VA, Ovchynnikov
VA, Zaitsev VN 1999. Coordination compounds of
Co (II), Ni(II), Mn(II), and Zn(II) with new
representative of carbacylamidophosphates–
potential anticancer drugs. 5th International
symposium on applied bioinorganic chemistry,
Greece.
- Bakkali F, Averbeck S, Averbeck D, Zhiri A, Baudoux
D, Idaomar M 2006. Antigenotoxic effects of three
essential oils in diploid yeast (Saccharomyces
cerevisiae) after treatments with UVC radiation, 8-
MOP plus UVA and MMS. Mutation Research, 606:
27-38.
- Bergman LW 2001. Growth and Maintenance of Yeast.
2001. Methods in Molecular Biology, Vol. 177, TwoHybrid
Systems: Methods and Protocols Edited by:
P. N. MacDonald © Humana Press Inc., Totowa, NJ.
- Braconi D, Sotgi M, Millucci L, Paffetti A, Tasso F,
Alisi C, Martini S, Rappuoli R, Lusini P, Rosa A,
Rossi C, Santucci A 2006. Comparative analysis of
the effects of locally used herbicides and their active
ingredients on a wild-type wine (Saccharomyces cerevisiae) strain. Journal of Agricural and Food
Chemistry, 54: 31633172.
- Braconi D, Bernardini G, Santucci A 2015.
Saccharomyces cerevisiae as a model in
ecotoxicological studies: A post-genomics
perspective. Journal of Proteomics, 137:19-34.
- Ceyhan G, Çelik C, Uruş S, Demirtaş İ, Elmastaş M,
Tümer M 2011. Antioxidant, electrochemical,
thermal, antimicrobial and alkane oxidation
properties of tridentate Schiff base ligands and
their metal complexes. Spectrochimica Acta Part A,
81: 184– 198.
- Chavan VL, Mehta BH 2011. X-ray, Thermal and
biological studies of Ru (III), Rh(III) and Pd(II)
schiff base metal complexes. Research Journal of
Chemistry and Environment, 15: 57-61.
- Cheeseman KH, Slater TF 1993. An in production to
free radical biochemistry. British Medical Bulletin,
49 (3): 481-93.
- Chohan ZH, Praveen M 2001. Synthesis,
characterization, coordination and antibacterial
properties of novel asymmetric 1,1′-disubstituted
ferrocene-derived Schiff-base ligands and their
Co(II), Cu(II) Ni(II) and Zn(II) complexes. Applied
Organometalic Chemistry, 15: 617-625.
- Chu Z, Huang W 2007. Syntheses and structures of two
new bis-N,O-bidentate schiff base ligands and their
respective copper(II) complexes with dinuclear
double-helical configuration. Journal of Molecular
Structure, 837: 15-22.
- Dikci İ 1999. Akut viral hepatitlerle interferon tedavisi
görmüs kronik viral hepatitlerde oksidatif stresin
arastırılması. Selçuk Üni. Tıp Fak. Biyokimya
Anabilim Dalı, Uzmanlık Tezi, 73s.
- Dolaz M, McKee V, Uruş S, Demir N, Sabik AE, Golcu
A, Tumer M 2010. Synthesis, structural
characterization, catalytic, thermal and
electrochemical investigations of bidentate Schiff
base ligand and its metal complexes.
Spectrochimica Acta A, 76: 174-181.
- Dominguez-Vera JM, Galvez N, Moreno JM, Colacio E
1998. Copper (II) complexes of two new oxamidate
bis-tetradentate schiff-base ligands. Polyhedron,
17: 2713-2718.
- Ellman GI 1959. Tissue sulfhydryl groups. Archives of
Biochemistry and Biophysics, 70-77.
- Esterbauer H, Gebicki J, Puhl H, Jgens G 1992. The
role of lipit peroxidation and antioxidants in
oxidative modification of LDL. Free Radical Biology
and Medicine, 13: 341-90.
- Garcia JJ, Reiter RJ, Guerrero JM, Escames G, Yu BP,
Oh CS 1997. Melatonin prevents changes in
microsomal membrane fluidity during induced lipid
peroxidation. FEBS Letters, 408: 297-300.
- Helmut S, 1976. Metal ions in biological systems,
Marcel Dekker Inc, New York, 2-50s.
- Izawa S, Inoue Y, Kimura, A 1995. Oxidative stress
response in yeast: effect of glutathione on adaption to hydrogen peroxide stress in Saccharomyces
cerevisia. FEBS Letters, 368: 73-76.
- Karlin KD, Tyekkerz L 1993. Bioorganic Chemistry of
Cupper. Chapman and Hill, NewYork.
- Kaya B 2003. Anti-genotoxic effect of ascorbic acid on
mutagenic dose of three alkylating agents. Turkish
Journal of Biology, 27: 241-246.
- Kelland LR, Barnard CF, Mellish KJ, Jones M,
Goddard PM, Valenti M, Bryant A, Murrer BA,
Harap KR 1994. A novel trans-platinum
coordination complex possessing in vitro and in vivo
antitumor activity. Cancer Research, 54: 5618-5622
- Kim C, Yoong-He L 1992. Synthesis and evaluation of
uracil-6-carboxaldehyde Schiff bases as potential
antitumor agents. Korean Journal of Medicinal
Chemistry, 2(1).
- Kirecci OA 2017. Saccharomyces cerevisiae’nın
gelişme ortamına ilave edilen ağır metallerin (Mn,
Mg, Cd, Fe) bazı biyokimyasal parametrelere
etkileri. KSU Doğa Bilimleri Dergisi, 20(3): 175-
184.
- Klayman DL, Scovill JP, Bartosevich JF, Bruce J 1983.
2-Acetylpyridine thiosemicarbazones.1-[l-(2-
Pyridyl) ethyl]-3-thiosemicarbazides as potential
antimalarial agents. Journal of Medicinal
Chemistry, 26-35.
- Kuduk J, Trynda L 1994. Impact of K 2 PtCl 6 on the
structure of human serum albumin and its binding
ability of heme and bilirubin. Journal of Inorganic
Biochemistry, 53: 4, 249-260.
- Lee JH, Choi IY, Kil IS, Kim SY, Yang ES, Park J 2001.
Protective role of superoxide dismutases against
ionizing radiation in yeast. Biochimica et
Biophysica Acta, 1526: 191–198.
- Li-Juan C, Fu-Ming M, Guang-Xing L 2009. Co (II)
Schiff base complexes on silica by sol–gel method as
heterogeneous catalysts for oxidative carbonylation
of aniline. Catalysis Communication, 10: 981-985.
- Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ
1951. Protein measurement with the folin-phenol
reagent. The Journal of Biochemistry, 193: 265-
277.
- Mathur S, Tabassum S 2006. New homodi- and
heterotrinuclear metal complexes of Schiff base
compartmental ligand: Interaction studies of copper
complexes with calf thymus DNA. Central
European Journal of Chemistry, 4: 502–522.
- Metzler CM, Cahill A, Metzler DE 1980. Equilibriums
and absorption spectra of Schiff bases. Journal of
The American Chemical Society, 102(19): 6075-
6082.
- Mirabelli CK, Hill DT, Faucette LF, McCabe FL,
Girard GR, Bryan DB, Sutton BM, Bartus JO,
Crooke ST, Johnson RK 1987. Antitumor activity of
bis(diphenylphosphino)alkanes, their gold(I)
coordination complexes, and related compounds.
Journal of Medicinal Chemistry, 30: 2181-90
- Mounika K, Anupama B, Pragathi J, Gyanakumari C
2010. Synthesis¸ characterization and biological
activity of a Schiff base derived from 3-ethoxy
salicylaldehyde and 2-amino benzoic acid and its
transition metal complexes. Journal of Scientific
Research, 2: 513-524.
- Mukherjee S, Samanta, S, Roy, B. C, Bhaumik, A 2006.
Efficient allylic oxidation of cyclohexene catalyzed
by immobilized Schiff base complex using peroxides
as oxidants. Applied Catalaysis A: General, 301: 79-
88.
- Niederhoffer EC, Timmons JH, Martel AG 1984.
Thermodynamics of oxygen binding in natural and
synthetic dioxygen complexes. Chemical Reviews,
84-137.
- Ohkawa H, Ohishi N, Yagi K 1979. Assay for lipid
peroxides in animal tissues by thiobarbituric acid
reaction. Analytical Biochemistry, 95: 351.
- Patel VK, Vasanwala AM, Jejurkar CR 1989.
Synthesis of mixed Schiff base complexes of Cu (II)
and Ni (II) and their spectral, magnetik and
antifungal studies. Indian Journal of Chemistry,
28A: 719-721.
- Penninckx M 2000. A short review on the role of
glutathione in the response of yeasts to nutritional,
environmental, and oxidative stresses. Enzyme and
Microbial Technology, 26: 737-742.
- Peralta RA, Neves A, Bortoluzzi AJ, dos Anjos A,
Xavier FR, Szpoganicz B, Terenzi H, Oliveira MCB,
Castellano E, GR, Friedermann, Mangrich, AS,
Novak MA 2006. New unsymmetric dinuclear Cu
(II)Cu(II) complexes and their relevance to
copper(II) containing metalloenzymes and DNA
cleavage. Journal of Inorganic Biochemistry, 100:
992-1004.
- Prashanthi Y, Raj S 2010. Synthesis and
characterization of transition metal complexes with
N,O; N,N and S,N-donor Schifff base ligands.
Journal of Scientific Research, 2: 114-126.
- Raman N, Muthuraj V, Ravichandran S,
Kulandaisamy, A 2003. Synthesis, characterization
and electrochemical behavior of Cu (II), Co(II),
Ni(II) and Zn(II) complexes derived from
acetylacetone and p-anisidine and their
antimicrobial activity. Proceedings of the Indian
Academy of Science, 115-161.
- Reiss A, Florea S, Caproiu T, Stanica N 2009.
Synthesis, characterization, and antibacterial
activity of some transition metals with the Schiff
base N-(2-furanylmethylene)-3-
aminodibenzofuran. Turkish Journal of Chemistry,
33: 775 – 783.
- Reddy KH, Reddy PS 2000. Nuclease activity of mixed
ligand complexes of copper (II) with heteroaromatic
derivatives and Picoline. Transition Metal
Chemistry, 25: 505-510.
- Rho YS, Kim SA, Jung JC, Shin CC, Chang SG 2002.
Anticancer cytotoxicity and nephro- toxicity of the new platinum (II) complexes containing
diaminocyclohexane and glycolic acid.
International Journal of Oncology, 20: 929-35.
- Sağlam N, Çolak A, Serbest K, Dülger S, Güner S,
Karaböcek S, Beldüz AO 2002. Oxidative cleavage
of DNA by homo- and heteronuclear Cu(II)-Mn(II)
complexes of an oxime-type ligand. Biometals, 15:
357- 365.
- Scovill JP, Klayman DL, Franchino F 1982. 2-
Acetylpyridine thiosemicarbazones. 4. Complexes
with transition metals as antimalarial and
antileukemic agents. Journal of Medicinal
Chemistry, 25: 1261.
- Serin S 1980. 1,3-difenil-2-tio-4,5-bis(hidroksimino)-
1,2,4,5-tetrahidroimidazol eldesi, geometrik
izomerleri, geçiş metalleri ile kompleks
formasyonları. KTÜ. Fen Bil. Ens., Kimya ABD,
Doktora Tezi, Trabzon.
- Silva AR, Wilson K, Clark JH, Freire C 2006. Covalent
attachment of chiral manganese (III) salen
complexes onto functionalised hexagonal
mesoporous silika and application to the
asymmetric epoxidation of alkenes. Microporous
and Mesoporous Materials, 91: 128-138.
- Shayma AS, Yang F, Abbas AS 2009. Synthesis and
characterization of mixed ligand complexes of 8-
hydroxyquinoline and o-hyroxybenzylidene-1-
phenyl-2,3-dimethyl-4-amino-3-pyrazolin-5-on with
Fe(II), Co(II), Ni(II) and Cu(II) ions. European
Journal of Scientific Research, 33: 702-709
- Şekeroğlu, ZA, Şekeroğlu V 2011. The in vitro alkaline
comet assay in genetic toxicology. The Journal of
Applied Behavioral Science, 5: 49-54.
- Takani M, Yajimab T, Masudac H, Yamauchi O 2002.
Spectroscopic and structural characterization of
copper (II) and palladium (II) complexes of a lichen
substance usnic acid and its derivatives. Possible
forms of environmental metals retained in lichens.
Journal of Inorganic Biochemistry, 91: 139-150.
- Tamarit J, Cabiscol E, Ros J 1998. Identification of the
major oxidatively damaged proteins in Escherichia
coli cells exposed to oxidative stres. Journal of
Biological Chemistry, 273: 3027 – 3032.
- Treshchalina EM, Konovalova AL, Presnov MA,
Chapurina LF, Belichuk NI 1979. Antitumor
properties of mixed coordination compounds of
copper (II) and alpha-amino acids. Doklady
Akademii Nauk, 248: 1273-6).
- Wang XW, Zheng YQ 2007. A dinuclear copper (II)
complex and a zigzagchain iron(II) polymer based
on the 4-antipyrine derived Schiff base ligands: The
hydroxylation and redox occurred under the
solvothermal conditions. Inorganic Chemistry
Communication, 10: 709-712.
- West DX, Pannel LK 1989. Transition metal ion
complexes of thiosemicarbazones derived from 2-
acetylpyridineN-oxide. II. The N-dimethyl
derivative. Transition Metal Chemistry, 14: 457-
462.
- Xu GJ, Yan SP, Liao DZ, Jiang ZH, Cheng P 2005. A
New Schiff Base Copper (II) Complex: Bis [N-(4-
hydroxysalicylidene)-N,N-diethylethylenediamineK
2 N,O]bis[(oxalato- K 2O,O)copper(II)]dihydrate.
Acta Crystallographica, E61: 933-935.
- You ZL, Zhu HL 2004. Syntheses, crystal structures,
and antibacterial activities of four Schiff base
complexes of copper and zinc. Zeitschrift für
Anorganische und Allgemeine Chemie, 630: 2754-
2760.
- Zeishen W, Zigi G, Zhenhuan Y 1990. Synthesis,
characterization and anticanser activity of L –
alanin Schiff base complexes of cooper ( II ), zinc(II
), and cobalt (II ). Inorganic Metal Organic
Chemistry, 20 (3): 335 – 344.