Bazı Schiff Bazlarının Saccharomyces cerevisiae BY4741 Kültür Ortamlarında Biyokimyasal Parametreler Üzerine Etkileri

Yeni sentezlenmiş bazı Schiff bazlarının kullanılmadan öncecanlılar üzerindeki biyokimyasal etkilerinin incelenmesi gerekir.Bu amaçla Saccharomyces cerevisiae en önemli hücre modeli içindeyer alır. S. cerevisiae’daki metabolik özellikler, yüksek yapılıorganizmalara benzerlik gösterdiği için elde edilen sonuçlar daparalellik göstermektedir.Çalışmada; yeni sentezlenmiş Schiff bazlarının S. cerevisiaeBY4741 kültür ortamlarında lipit peroksidasyon (MDA), totalprotein ve glutatyon analizleri yapılmıştır. Bu amaçla; deneydekullanılan S. cerevisiae BY4741’in gelişimi ve çoğalması için YEDPbesiyeri ortamı hazırlandı. Uygulama grupları için; schiff bazlarınher birinden 2ppm, 4 ppm ve 8 ppm olacak şekilde kültür ortamınailave edildi. Elde edilen süpernatant ile; GSH,total protein ve MDAanalizleri yapıldı.Lipid peroksidayon sonuçları kontrol grubu ile karşılaştırıldığındaschiff bazı gruplarının lipid peroksidasyon önleme etkilerininolmadığı belirlenirken, yeni sentezlenen schiff bazlarının kontrolgrubuna göre glutatyon ve total protein miktarlarının oldukçabelirgin düzeyde yüksek olduğu saptandı (p

The Effects of Some Schiff Bases on Biochemical Parameters in Saccharomyces cerevisiae BY4741 Cultural Environments

Biochemical effects of newly synthesized some Schiff bases on living creatures need to be investigated before their use. For this purpose, Saccharomyces cerevisiae is involved in the most important cell model. Because metabolic properties of S. cerevisiae are similar to the highly organized organisms, the results show parallelism, as well. In the present study; lipid peroxidation (MDA), total protein, and glutathione analyses of newly synthesized Schiff bases were analysed in S. cerevisiae BY4741 culture media. For this purpose, YEDP medium was prepared for development and growth of S. cerevisiae BY4741 used in the experiment. In the study, 2 ppm, 4 ppm, and 8 ppm from each of Schiff bases were added in to culture medium for application groups. GSH, total protein and MDA analyses were carried out with the obtained supernatant. As the results of lipid peroxidation were compared to the control group, it was concluded that the Schiff base groups had no lipid peroxidation inhibition effects, and the newly synthesized schiff bases were found to have significantly greater amounts of glutathione and total protein than the control group (p

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KSÜ Tarım ve Doğa Dergisi-Cover
  • ISSN: 2619-9149
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 2018
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