İbrahim TURAN, Yüksel ALİYAZICIOĞLU, Sema MISIR, Selim DEMİR

Dorycnium pentaphyllum Ekstraktının İnsan Meme, Karaciğer ve Akciğer Kanseri Hücrelerindeki Seçici Sitotoksik Etkisi

Fabaceae ailesine mensup bitkilerin sitotoksik etkilerini konu alan bazı çalışmalar bulunmakla birlikte, Dorycnium pentaphyllum'un sitotoksik etkisi ile ilgili sınırlı araştırma bulunmaktadır. Bu çalışmanın amacı, D. pentaphyllum ekstraktının antioksidan özelliklerinin ve çeşitli insan kanser hücreleri üzerindeki sitotoksik etkisini belirlenmesidir. Ekstraktın toplam fenolik içeriği kolorimetrik yöntem kullanılarak belirlendi. Ekstraktın insan akciğer (A549), karaciğer (HepG2) ve meme (MCF-7) kanser hücreleri ile normal insan fibroblast hücreleri üzerindeki sitotoksik etkisi MTT analizi kullanılarak değerlendirildi. Ekstraktın toplam fenolik içerik değeri g örnek başına 140.3±1.1 mg gallik asit eşdeğeri olarak bulundu. Ekstraktın çalışılan tüm kanser hücre serileri üzerinde normal fibroblast hücrelerine göre seçici sitotoksik etki gösterdiği belirlendi ve IC50 değerleri 100.4-298.5 μg/mL arasında hesaplandı. Bu çalışma D. pentaphyllum ekstraktının kanser hücre serileri üzerindeki sitotoksik etkisini ortaya koyan ilk çalışmadır. D. pentaphyllum'un fitokimyasal uygulamaları kanser tedavisinde umut verici yaklaşımları temsil edebilir.

Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells

Although some studies have evaluated the cytotoxic activity ofdifferent Fabaceae species, there has been only limited research intothe cytotoxic effect of Dorycnium pentaphyllum. The purpose of thisstudy was to determine the antioxidant properties and cytotoxic effectof D. pentaphyllum extract on various human cancer cells. The totalphenolic content (TPC) of the extract was determined usingcolorimetric method. The cytotoxic effect of the extract on human lung(A549), liver (HepG2), and breast (MCF-7) cancer cells and a normalhuman fibroblast cells was assessed using the MTT assay. TPC valueof extract was found as 140.3±1.1 mg gallic acid equivalent per gsample. Extract showed selective cytotoxicity on all studied cancercells compared to normal fibroblast cells, and the IC50 values of theextract in the cancer cells range from 100.4 to 298.5 μg/mL. This is thefirst study to reveal the cytotoxic effect of D. pentaphyllum extract oncancer cell lines. Phytomedical applications of D. pentaphyllum mayrepresent promising approaches in the treatment of cancer.

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