Esra KAYA, Murat ARAL, ZERİFE ORHAN, Kezban Tülay YALÇINKAYA, Hacer UĞURLU

Comparison of Conventional PCR Method With Cefoxitin Disc Diffusion, Automated System and Isolation on Chromogenic MRSA Medium Methods for The Detection of Meticillin Resistance In Staphylococcus aureus Strains Isolated From Various Clinical Specimens

Meticillin-resistant Staphylococcus aureus (MRSA) is one of the mostimportant infectious agents. Therefore, fast and accurate diagnosisof MRSA is utmost important. Although mecA gene detection by PCRis reference method, conventional methods are preferred in routinepractices due to simplicity. As conventional methods last 48-96hours, several chromogenic media have been developed. Ourobjective was to compare the methods used for meticillin resistancedetection with PCR. Forty-eight S. aureus strains isolated fromvarious clinical specimens were included. Of the 48 S. aureus strains,19 were mecA gene-positive and 29 were negative. mecA genepositive 19 strains were also meticillin-resistant by automatedsystem and disk diffusion. On chromogenic agar, 15 of 19 MRSAstrains were meticillin-resistant and 4 were meticillin-sensitive.Twentynine mecA gene-negative strains were susceptible tometicillin by automatized system and disk diffusion. Among 29 mecAgene-negative MSSA strains inoculated on chromogenic agar, 17were methicillin-resistant. According to our study, chromogenicmedia would be ineffective to detected to meticillin-resistancebecause of low sensitivity and specificity in routine.

Çeşitli Klinik Örneklerden Izole Edilen Staphylococcus aureus Suşlarında Metisilin Direncinin Araştırılmasında Konvansiyonel PCR Yöntemi ile Sefoksitin Disk Difüzyon, Otomatize Sistem ve Chromogenic MRSA Agar Yöntemlerinin Karşılaştırılması

Metisiline dirençli Staphylococcus aureus (MRSA) en önemli enfeksiyöz ajanlardan biridir. Bu nedenle MRSA'nın hızlı ve doğru tanısı çok önemlidir. PCR ile mecA gen tespiti referans metodu olmakla birlikte, basit olmasından dolayı rutin uygulamada geleneksel yöntemler tercih edilmektedir. Geleneksel yöntemler ile tanının koyulması 48-96 saat sürdüğü için çeşitli kromojenik besiyerleri geliştirilmiştir. Çalışmamızdaki amacımız metisilin direncinin tespitinde kullanılan çeşitli yöntemleri PCR ile karşılaştırmaktır. Çeşitli klinik örneklerden izole edilen 48 S. aureus suşu çalışmamıza dahil edilmiştir.48 S.aureus suşunun 19'u mecA gen-pozitif ve 29'u negatiftir. mecA gen-pozitif 19 suş da otomatik sistem ve disk difüzyonu ile dirençli bulunmuştur. Kromojenik agarda, 19 MRSA suşunun 15'i metisilin dirençli ve 4'ü duyarlı bulunmuştur.29 mecA gen-negatif suş, otomatik sistem ve disk difüzyonu ile duyarlı bulunmuştur. mecA geni negatif olan 29 MSSA suşunun kromojenik agarda 17’si dirençli olarak değerlendirmiştir.

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