Abdussamat GÜZEL, MAHFUZ ELMASTAŞ

Antioxidant Activity, Isolation and Identification of Some Chemical Constituents of Sphaerophysa kotschyana

Sphaerophysa kotschyana, an endemic plant belonging to Fabaceae,is distributed around the Tuz Lake in the Konya region. In this study,methanolic extract of S. kotschyana was evaluated for antioxidantcapacity. According to obtained data; free radical scavenging capacity(DPPH●) and reducing power activity were observed to be lower thanstandard antioxidants, cation radical scavenging activity (ABTS●+)was similar to the standard antioxidants. Total phenolic compoundwas found in leaf-stem and fruit 0.357 mg GAE/kg DW and 0.006 mgGAE/kg DW, respectively. D-pinitol and sucrose were isolated andidentified by chromatographic and spectroscopic techniques.

Sphaerophysa kotschyana Bitkisinin Antioksidan Aktivitesi ve Bazı Kimyasal Bileşenlerinin Izolasyonu ve Belirlenmesi

Fabaceae'ye ait endemik bir bitki olan Sphaerophysa kotschyana, Konya bölgesindeki Tuz Gölü çevresinde dağılım gösterir. Bu çalışmada, S. kotschyana bitkisinden elde edilen metanol ekstrelerden antioksidan kapasitesi değerlendirildi. Elde edilen antioksidan aktivite sonuçlarına göre; serbest radikal (DPPH●) giderme aktivitesi ve indirgeme gücü aktivitesi standart antioksidanlara göre düşük olduğu gözlemlenirken, katyon radikal (ABTS●+) giderme aktivitesi standart antioksidanlara benzer bir aktivite göstermektedir. Toplam fenolik bileşiklerin tayininde sırasıyla yaprak-sapı ve meyvede, 0,357 mg GAE / kg kuru bitki ve 0,006 mg GAE / kg kuru bitki olarak bulunmuştur. D-pinitol ve sukroz kromatografik ve spektroskopik teknikler ile izole edildi ve yapısı aydınlatıldı.

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