Ayhan TANYELİ, İlhami GÜLÇİN, Ersen ERASLAN, Fazile Nur EKİNCİ AKDEMİR, Mustafa Can GÜLER, Saime ÖZBEK SEBİN

İntestinal İskemi/Reperfüzyon Hasarının Hafifletilmesinde p-Kumarik Asit’in Rolü

GİRİŞ ve AMAÇ: Bu çalışma intestinal iskemi/reperfüzyon (İ/R) hasarına karşı p-kumarik asit’ in koruyucu rolünü araştırmak amacıyla planlandı. YÖNTEM ve GEREÇLER: Bu amaçla, çalışmamızda deneysel intestinal İ/R hasar modeli oluşturuldu. p-Kumarik asit ve İ/R gruplarında, süperior mezenterik arter 1 saat süreyle klemplendi. Bu sürenin sonunda klemp açıldı ve 2 saat süreyle reperfüzyona izin verildi.2 saatin sonunda, sıçanlar sakrifiye edildi ve intestinal doku örnekleri hızlıca toplandı. BULGULAR: İ/R’ye bağlı olarak şiddetli oksidatif hasardan dolayı MPO aktivitesi, MDA seviyesi, TOS and OSI değerlerinin arttığı belirlendi. İlaveten İ/R grubunda TAC değeri ve SOD aktivitesi azaldı. Ayrıca, İ/R + p-kumarik asit (50 ve 100 mg/kg) gruplarında MPO aktivitesi, MDA seviyesi, TOS and OSI değerleri azalırken TAC değeri ve SOD aktivitesi arttı. Dahası, p-kumarik asit 50 mg/kg grubu ile karşılaştırıldığı zaman p-kumarik asit 100 mg/kg grubunda TAC değeri ve SOD aktivitesi daha fazla artarken OSI ve TOS değerleri daha fazla azaldı. TARTIŞMA ve SONUÇ: p-Kumaraik asit’ in farklı dozları deney hayvanlarında intestinal İ/R (1saat: 2saat) hasara karşı koruyucu etki gösterdiği söylenilebilir.

Role of p-Coumaric Acid in Alleviating of The Intestinal Ischemia/Reperfusion Injury

INTRODUCTION: This study was planned to investigate theprotective role of p-coumaric acid against intestinalischemia/reperfusion (I/R) injury.METHODS: For this purpose, an experimental intestinal I/Rmodel was established in our study. In the p-coumaric acid andI/R groups, superior mesenteric artery was clamped for 1 h.Then, the clamp was removed and reperfusion was allowed for2 h. At the end of 2h, rats were sacrificed and intestinal tissuesamples were collected rapidly.RESULTS: It was determined that MPO activity, MDA levels,TOS and OSI values increased due to severe oxidative damagedepending on I/R. In addition, TAC value and SOD activitydecreased in the I/R group. Also, TAC value and SOD activityincreased while TOS, OSI values, MPO activity and MDA levelwere reducing in the I/R + p-coumaric acid (50 and 100 mg/kgbw.) groups. Moreover, in the p-coumaric acid 100 mg/kggroup, TAC value and SOD activity more increased and OSI,TOS values more decreased compared with p-coumaric acid50 mg/kg group.DISCUSSION AND CONCLUSION: It can say that thedifferent doses of the p-coumaric acid demonstrated protectiveeffects against intestinal I/R (1hour: 2hour) injury inexperimental animals.

PDF

___

1. Campos VF, Miranda-Ferreira R, Taha NSA, Teixeira GD, Souza WTI, Carmo CEF, et al. Atenolol to Treat Intestinal Ischemia and Reperfusion in Rats. Transplantation Proceedings. 2012;44(8):2313-6.
2. Chang JX, Chen S, Ma LP, Jiang LY, Chen JW, Chang RM, et al. Functional and morphological changes of the gut barrier during the restitution process after hemorrhagic shock. World Journal of Gastroenterology. 2005;11(35):5485-91.
3. Petrat F, Swoboda S, de Groot H, Schmitz KJ. Quantification of Ischemia-Reperfusion Injury to the Small Intestine Using a Macroscopic Score. Journal of Investigative Surgery. 2010;23(4):208-17.
4. Grootjans J, Lenaerts K, Buurman WA, Dejong CHC, Derikx JPM. Life and death at the mucosal-luminal interface: New perspectives on human intestinal ischemia-reperfusion. World Journal of Gastroenterology. 2016;22(9):2760-70.
5. Cerqueira NF, Hussni CA, Yoshida WB. Pathophysiology of mesenteric ischemia/reperfusion: a review. Acta Cir Bras. 2005;20(4):336-43.
6. Yasuhara H. Acute mesenteric ischemia: The challenge of gastroenterology. Surgery Today. 2005;35(3):185-95.
7. Zhao JP, Yu SJ, Tong LQ, Zhang F, Jiang X, Pan S, et al. Oxymatrine attenuates intestinal ischemia/reperfusion injury in rats. Surgery Today. 2008;38(10):931-7.
8. Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol. 2000;190(3):255-66.
9. Oztürk Sarıkaya SB, Topal F, Şentürk M, Gülçin İ, Supuran CT. In vitro inhibition of αcarbonic anhydrase isozymes by some phenolic compounds. Bioorganic and Medicinal Chemistry Letters. 2011;21 (14):4259-62.
10. Sarikaya SBO, Gulcin I, Supuran CT. Carbonic Anhydrase Inhibitors: Inhibition of Human Erythrocyte Isozymes I and II with a Series of Phenolic Acids. Chem Biol Drug Des. 2010;75(5):515-20.
11. Ferguson LR, Lim IF, Pearson AE, Ralph J, Harris PJ. Bacterial antimutagenesis by hydroxycinnamic acids from plant cell walls. Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2003;542(1-2):49-58.
12. Pragasam SJ, Venkatesan V, Rasool M. Immunomodulatory and Anti-inflammatory Effect of p-Coumaric Acid, a Common Dietary Polyphenol on Experimental Inflammation in Rats. Inflammation. 2013;36(1):169-76.
13. Sharma SH, Chellappan DR, Chinnaswamy P, Nagarajan S. Protective effect of p-coumaric acid against 1,2 dimethylhydrazine induced colonic preneoplastic lesions in experimental rats. Biomedicine & Pharmacotherapy. 2017;94:577-88.
14. Ekinci-Akdemir FN, Gulcin I, Gursul C, Alwasel SH, Bayir Y. Effect of P-Coumaric Acid against Oxidative Stress Induced by Cisplatin in Brain Tissue of Rats. J Anim Plant Sci. 2017;27(5):1560-4.
15. Ekinci Akdemir FN, Albayrak M, Calik M, Bayir Y, Gulcin I. The Protective Effects of pCoumaric Acid on Acute Liver and Kidney Damages Induced by Cisplatin. Biomedicines. 2017;5(2).
16. Prince PSM, Roy AJ. p-Coumaric acid attenuates apoptosis in isoproterenol-induced myocardial infarcted rats by inhibiting oxidative stress. International Journal of Cardiology. 2013;168(4):3259-66.
17. Ohkawa H, Ohishi N, Yagi K. Assay for Lipid Peroxides in Animal-Tissues by Thiobarbituric Acid Reaction. Analytical Biochemistry. 1979;95(2):351-8.
18. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry. 2004;37(4):277-85.
19. Harma M, Harma M, Kocyigit A, Erel O. Increased DNA damage in patients with complete hydatidiform mole. Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2005;583(1):49-54.
20. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol. 1982;78(3):206-9.
21. Sun Y, Oberley LW, Li Y. A Simple Method for Clinical Assay of Superoxide-Dismutase. Clin Chem. 1988;34(3):497-500.
22. Mallick IH, Yang WX, Winslet MC, Seifalian AM. Ischemia-reperfusion injury of the intestine and protective strategies against injury. Digestive Diseases and Sciences. 2004;49(9):1359- 77.
23. Mester A, Magyar Z, Sogor V, Tanczos B, Stark Y, Cherniavsky K, et al. Intestinal ischemiareperfusion leads to early systemic microrheological and multiorgan microcirculatory alterations in the rat. Clinical Hemorheology and Microcirculation. 2018;68(1):35-44.
24. Ross S, Foex P. Protective effects of anaesthetics in reversible and irreversible ischaemiareperfusion injury. British Journal of Anaesthesia. 1999;82(4):622-32.
25. Granger DN, Korthuis RJ. Physiologic mechanisms of postischemic tissue injury. Annu Rev Physiol. 1995;57:311-32.
26. Guneli E, Cavdar Z, Islekel H, Sarioglu S, Erbayraktar S, Kiray M, et al. Erythropoietin protects the intestine against ischemia/reperfusion injury in rats. Molecular Medicine. 2007;13(9- 10):509-17.
27. Bursal E, Gülçin İ. Polyphenol contents and in vitro antioxidant activities of lyophilized aqueous extract of kiwifruit (Actinidia deliciosa). Food Research International. 2011;44(5):1482-9.
28. Gulcin I. Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012;86(3):345-91.
29. Grogaard B, Parks DA, Granger DN, Mccord JM, Forsberg JO. Effects of Ischemia and Oxygen Radicals on Mucosal Albumin Clearance in Intestine. American Journal of Physiology. 1982;242(5):G448-G54.
30. Gulcin I. Antioxidant properties of resveratrol: A structure-activity insight. Innovative Food Science & Emerging Technologies. 2010;11(1):210-8.
31. Moslen MT. Reactive oxygen species in normal physiology, cell injury and phagocytosis. Adv Exp Med Biol. 1994;366:17-27.
32. Parks DA, Granger DN. Ischemia-induced vascular changes: role of xanthine oxidase and hydroxyl radicals. Am J Physiol. 1983;245(2):G285- 9.
33. Gulcin I. Antioxidant and antiradical activities of L-carnitine. Life Sciences. 2006;78(8):803-11.
34. Gulcin I. Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology. 2006;217(2-3):213-20.
35. Barut I, Tarhan OR, Kapucuoglu N, Sutcu R, Akdeniz Y. Lamotrigine reduces intestinal I/R injury in the rat. Shock. 2007;28(2):202-6.
36. Yildiz Y, Kose H, Cecen S, Ergin K, Demir EM, Serter M. Protective Effects of Leflunomide on Intestinal Ischemia-Reperfusion Injury Leflunomide against intestinal ischemia-reperfusion. Digestive Diseases and Sciences. 2010;55(2):245-52.
37. Terzi A, Coban S, Yildiz F, Ates M, Bitiren M, Taskin A, et al. Protective Effects of Nigella Sativa on Intestinal Ischemia-Reperfusion Injury in Rats. Journal of Investigative Surgery. 2010;23(1):21-7.
38. Demirpence O, Sevim B, Yildirim M, Nurlu NA, Mert D, Evliyaoglu O. Serum paraoxonase, TAS, TOS and ceruloplasmin in brucellosis. International Journal of Clinical and Experimental Medicine. 2014;7(6):1592-7.
39. Yildiz Y, Serter M, Ek RO, Ergin K, Cecen S, Demir EM, et al. Protective Effects of Caffeic Acid Phenethyl Ester on Intestinal IschemiaReperfusion Injury. Digestive Diseases and Sciences. 2009;54(4):738-44.
40. Zhang Z, Pan C, Wang HZ, Li YX. Protective Effects of Osthole on Intestinal IschemiaReperfusion Injury in Mice. Experimental and Clinical Transplantation. 2014;12(3):246-52.
41. Tanyeli A, Ekinci Akdemir FN, Eraslan E, Güler MC, Topdağı Ö. The Effect of Evodiamine Against Intestinal Tissue Injury Induced By Mesenteric Ischemia-Reperfusion: Role of Oxidative Stress. Bozok Med J 2019;9(1):113-8.
42. Augustin AJ, Goldstein RK, Milz J, Lutz J. Influence of anti-inflammatory drugs and free radical scavengers on intestinal ischemia induced oxidative tissue damage. Adv Exp Med Biol. 1992;316:239-51.
43. Ogura W, Itagaki S, Kurokawa T, Noda T, Hirano T, Mizuno S, et al. Protective effect of lutein on ischemia-reperfusion injury in rat small intestine. Biological & Pharmaceutical Bulletin. 2006;29(8):1764-6.