Ayhan TANYELİ, Fazile Nur EKİNCİ AKDEMİR, Ersen ERASLAN, Mustafa Can GÜLER, Saime ÖZBEK SEBİN, İlhami GÜLÇİN

Role of p-Coumaric Acid in Alleviating of The Intestinal Ischemia/Reperfusion Injury

INTRODUCTION: This study was planned to investigate the protective role of p-coumaric acid against intestinal ischemia/reperfusion (I/R) injury. METHODS: For this purpose, an experimental intestinal I/R model was established in our study. In the p-coumaric acid and I/R groups, superior mesenteric artery was clamped for 1 h. Then, the clamp was removed and reperfusion was allowed for 2 h. At the end of 2h, rats were sacrificed and intestinal tissue samples were collected rapidly. RESULTS: It was determined that MPO activity, MDA levels, TOS and OSI values increased due to severe oxidative damage depending on I/R. In addition, TAC value and SOD activity decreased in the I/R group. Also, TAC value and SOD activity increased while TOS, OSI values, MPO activity and MDA level were reducing in the I/R + p-coumaric acid (50 and 100 mg/kg bw.) groups. Moreover, in the p-coumaric acid 100 mg/kg group, TAC value and SOD activity more increased and OSI, TOS values more decreased compared with p-coumaric acid 50 mg/kg group. DISCUSSION AND CONCLUSION: It can say that the different doses of the p-coumaric acid demonstrated protective effects against intestinal I/R (1hour: 2hour) injury in experimental animals.

İntestinal İskemi/Reperfüzyon Hasarının Hafifletilmesinde p-Kumarik Asit’in Rolü

GİRİŞ ve AMAÇ: Bu çalışma intestinal iskemi/reperfüzyon (İ/R) hasarına karşı p-kumarik asit’ in koruyucu rolünü araştırmak amacıyla planlandı. YÖNTEM ve GEREÇLER: Bu amaçla, çalışmamızda deneysel intestinal İ/R hasar modeli oluşturuldu. p-Kumarik asit ve İ/R gruplarında, süperior mezenterik arter 1 saat süreyle klemplendi. Bu sürenin sonunda klemp açıldı ve 2 saat süreyle reperfüzyona izin verildi.2 saatin sonunda, sıçanlar sakrifiye edildi ve intestinal doku örnekleri hızlıca toplandı. BULGULAR: İ/R’ye bağlı olarak şiddetli oksidatif hasardan dolayı MPO aktivitesi, MDA seviyesi, TOS and OSI değerlerinin arttığı belirlendi. İlaveten İ/R grubunda TAC değeri ve SOD aktivitesi azaldı. Ayrıca, İ/R + p-kumarik asit (50 ve 100 mg/kg) gruplarında MPO aktivitesi, MDA seviyesi, TOS and OSI değerleri azalırken TAC değeri ve SOD aktivitesi arttı. Dahası, p-kumarik asit 50 mg/kg grubu ile karşılaştırıldığı zaman p-kumarik asit 100 mg/kg grubunda TAC değeri ve SOD aktivitesi daha fazla artarken OSI ve TOS değerleri daha fazla azaldı. TARTIŞMA ve SONUÇ: p-Kumaraik asit’ in farklı dozları deney hayvanlarında intestinal İ/R (1saat: 2saat) hasara karşı koruyucu etki gösterdiği söylenilebilir.

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