Fatih AVDATEK, Ruhi TÜRKMEN, Beste ÇİL, Muhammed Enes İNANÇ, Durmuş ORAKÇI, Umut TAŞDEMİR, Pürhan Barbaros TUNCER, Deniz YENİ

Yeşil Çay Ekstraktı İlavesinin Boğa Sperması Dondurulmasına Etkisi

Bu çalışmanın amacı yeşil çay ekstraktı olan kateşinin (CT) tris sulandırıcısına eklenerek boğa sperma dondurulmasında dondurma çözdürme sonucu sperma kalitesinin incelenmesidir. Ejakülatlar Holstein boğalardan alınarak eşit beş kısma ayrıldı ve farklı oranlarda (5, 10, 25 and 50 μg/mL) CT içeren ve içermeyen (kontrol) sulandırıcı ile sulandırıldı. Bütün örnekler 4°C’de 4 saat ekilibrasyona bırakıldı ve daha sonra otomatik dondurma makinası ile donduruldu. Çözüm sonu spermatozoa motilitesi ve kinetik parametreler sperma analiz sistemi ile değerlendirildi. DNA bütünlüğü tek hücre jel elektroforezi, anormal spermatozoa oranı sıvı fiksasyon testi ile, lipit peroksidasyon seviyesi ise kolorimetrik olarak ölçüldü. CT ilavesinin motilite ve kinetik parametreleri olumlu olarak etkilemediği görüldü. Fakat CT10 ve 25 ve 50 gruplarında kontrol grubuna göre daha yüksek morfolojik bütünlük tespit edildi (P

The Effect of Green Tea Extract Supplementation in Bull Semen Cryopreservation

This study aims to investigate the effect of catechin (CT), green tea extract, as a supplement to Tris extender on semen quality parameters infrozen-thawed of bull sperm. Ejaculates were taken with artificial vagina from Holstein bulls and divided equal five aliquots, diluted to containingdifferent amounts of CT (5, 10, 25 and 50 μg/mL) and no-additive (control). All samples were equilibrated at 4°C for 4 h and were frozen using adigital freezing machine. Post-thawed sperm motility and kinetic parameters were determined using the sperm analyser system. SpermatozoaDNA integrity was evaluated with the single cell gel electrophoresis, abnormal spermatozoa rate was evaluated by fluid fixation test and lipidperoxidation status was evaluated colorimetrically. CT supplementation did not improve motility and kinetic parameters. However, the highermorphological integrity was detected in CT10, 25 and 50 groups compared to control (P

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