Inhibin-βA (INHBA) could feedback suppress synthesis and secretion of follicle-stimulating hormone (FSH), which correlates with the litter size of sheep. In this study, the inhibin-βA subunit was used as a candidate gene, and Kazakh sheep was used as a test object. The polymorphism of the gene was detected by PCR-SSCP method and its association with litter size was analyzed. The results showed that there were three polymorphisms in the exon 5’UTR region (primer 0-2), for which AA, AB and BB were detected in Kazakh sheep. The correlation analysis showed that genotype AA had 0.13 (P
İnhibin-βA (INHBA), folikül stimule edici hormon (FSH) sentez ve salınımını baskılayabilir ki bu durum koyunlarda yavru sayısı ile ilişkili olabilir. Bu çalışmada, inhibin-βA geni aday gen olarak değerlendirilerek Kazak koyunları çalışma materyali olarak kullanıldı. Genin polimorfizmi PCR-SSCP metodu ile belirlendi ve yavru sayısı ile ilişkisi analiz edildi. Elde edilen sonuçlar, Kazak koyununda ekzon 5’UTR bölgesinde AA, AB ve BB için üç polimorfizmin bulunduğunu gösterdi (primer 0-2). Korelasyon analizi, Kazak koyununda genotip AA’da 0.13 kuzu bulunduğunu (P
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1. McCullagh DR: Dual endocrine activity of the testes. Science, 76, 19-20, 1932. DOI: 10.1126/science.76.1957.19
2. Robertson DM, Foulds LM, Leversha L, Morgan FJ, Hearn MTW, Burger HG, Wettenhall REH, Kretser DM: Isolation of inhibin from bovine follicular fluid. Biochem Biophys Res Commun, 126, 220-226, 1985. DOI: 10.1016/0006-291X(85)90594-7
3. Ling N, Ying SY, Ueno N, Esch F, Denoroy L, Guillemin R: Isolation and partial characterization of a Mr 32.000 protein with inhibin activity from porcine follicular fluid. Proc Natl Acad Sci USA, 82, 7217-7221, 1985. DOI: 10.1073/pnas.82.21.7217
4. Si T, Lu Y, Li FL, Jiang L, Pei RZ, Zhou JX: High expression of INHBA is an adverse prognostic factor for de novo acute myeloid leukemia. Leuk Lymphoma, 59, 114-120, 2018. DOI: 10.1080/10428194.2017.1324157
5. Teh APP, Izzati UZ, Mori K, Fuke N, Hirai T, Kitahara G, Yamaguchi R: Histological and immunohistochemical evaluation of granulosa cells during different stages of folliculogenesis in bovine ovaries. Reprod Domest Anim, 53, 569-581, 2018. DOI: 10.1111/rda.13132
6. Mann GE, Campbell BK, McNeilly AS, Baird DT: The role of inhibin and oestradiol in the control of FSH secretion in the sheep. J Endocrinol, 133, 381-391, 1992. DOI: 10.1677/joe.0.1330381
7. Zi XD, Xu HW, Wang Y: Variation in sequences and mRNA expression levels of inhibin subunits alpha (INHA) and beta A (INHBA) genes between prolific and nonprolific goat breeds. Mol Reprod Dev, 79, 238-238, 2012. DOI: 10.1002/mrd.22001
8. Leyhe B, Hiendleder S, Jaeger C, Wassmuth R: Pronounced differences in the frequency of TaqI βA-inhibin alleles between sheep breeds with different reproductive performance. Anim Genet, 25, 41-43, 1994. DOI: 10.1111/j.1365-2052.1994.tb00446.x
9. Knox RV, Vatzias G, Naber CH, Zimmerman DR: Plasma gonadotropins and ovarian hormones during the estrous cycle in high compared to low ovulation rate gilts. J Anim Sci, 81, 249-260, 2003. DOI: 10.2527/ 2003.811249x
10. Hu S, Ni W, Sai W, Zhang H, Cao X, Qiao J, Sheng J, Guo F, Chen C: Sleeping Beauty-mediated knockdown of sheep myostatin by RNA interference. Biotechnol Lett, 33, 1949-1953, 2011. DOI: 10.1007/s10529- 011-0667-8
11. Li D, Duan M, Feng Y, Geng LL, Li XQ, Zhang WG: MiR-146a modulates macrophage polarization in systemic juvenile idiopathic arthritis by targeting INHBA. Mol Immunol, 77, 205-212, 2016. DOI: 10.1016/j.molimm.2016.08.007
12. Kristensen SG, Andersen K, Clement CA, Stephen F, Kate H, Claus YA: Expression of TGF-beta superfamily growth factors, their receptors, the associated smads and antagonists in five isolated size-matched populations of pre-antral follicles from normal human ovaries. Mol Hum Reprod, 20 (4): 293-308, 2014. DOI: 10.1093/molehr/gat089
13. Yang J, Li X, Cao YH, Pokharel K, Hu XJ, Chen ZH, Xu SS, Peippo J, Honkatukia M, Kantanen J, Li MH: Comparative mRNA and miRNA expression in european mouflon (Ovis musimon) and sheep (Ovis aries) provides novel insights into the genetic mechanisms for female reproductive success. Heredity, 122 (2): 172-186, 2019. DOI: 10.1038/ s41437-018-0090-1
14. Chu MX, Xiao CT, Fu Y, Fang L, Ye SC: PCR-SSCP polymorphism of inhibin βA gene in some sheep breeds. Asian-Australas J Anim Sci, 20 (7): 1023-1029, 2007.
15. Chu MX, Peng ZL, Chen HQ, Zhang YJ, Fang L, Di R, Cao GL, Feng T, Li N: Polymorphism in exon 2 of INHBB gene and its relationship with litter size in Jining Grey goats. Anim Sci Pap Rep, 30 (1): 57-63, 2012.
16. Han L, Wu C, Riaz H, Bai L, Chen J, Zhen Y, Guo A, Yang L: Characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference. PLoS One, 8:e74596, 2013. DOI: 10.1371/journal.pone.0074596
17. Dan X, Liu X, Han Y, Liu Q, Yang L: Effect of the novel DNA vaccine fusing inhibin α (1-32) and the RF-amide related peptide-3 genes on immune response, hormone levels and fertility in Tan sheep. Anim Reprod Sci, 164, 105-110, 2016. DOI: 10.1016/j.anireprosci.2015.11.018