Bo HOU, Ru-jing CHEN, Qiu-yong CHEN, Xue-min WU, Yong-liang CHE, Chen-yan WANG, Lun-jiang ZHOU, Long-bai WANG

Farklı Domuz Viral Diyare Aşı Kombinasyonları İle İmmunizasyon Sonrası PED IgG ve IgA, TGE IgG ve PoR IgG Antikor Seviyelerindeki Farklılıkların Araştırılması

Domuz viral diyareyi önlemek için iyi antikor seviyesi sağlayan bir aşı kombinasyonunu belirlemeye ihtiyaç bulunmaktadır. Bu çalışmada, farklı aşı kombinasyonlarının domuz epidemik diyare, domuz bulaşıcı gastroenteritisi ve domuz rotavirus IgG seviyeleri ile domuz epidemik diyare IgA seviyesine etkilerini araştırmak amacıyla 30 gebe domuz 5’i immunize grup 1’i kontrol olmak üzere altı gruba ayrıldı. Antikor seviyeleri ELISA ile belirlendi. Elde edilen sonuçlar, domuz epidemik diyare ve domuz bulaşıcı gastroenteritisi serum IgG seviyeleri ile meme sütünde domuz epidemik diyare IgA seviyesinin “PT+PT*” aşı kombinasyonu grubunda diğerlerinden daha yüksek olduğunu ve “PTR”yi içeren aşı kombinasyonunun domuzları domuz rotavirus IgG antikoru üretmek üzere stimüle ettiğini gösterdi. Bu sonuçlar “PT+PT*” aşı kombinasyonunun domuz viral diyareyi önlemede en iyi olduğunu ve “PTR+PT*”in domuz rotavirus enfeksiyon riski bulunan bölgelerde bir alternatif olabileceğini gösterdi. Çalışma sonucunda domuz viral diyarenin bölgesel epidemik durumuna göre domuz çiftliklerinin immunizasyon seçiminde bulunması önerilir.

Study on the Antibody Level Differences of PED IgG and IgA, TGE IgG and PoR IgG After Immunization with Different Porcine Viral Diarrhea Vaccine Combinations

To prevent porcine viral diarrhea, a vaccine combination that can provide good antibody levels needs to be determined. In this study, we screened30 pregnant sows divided into six experimental groups, namely, five immunized groups and one control group, to investigate the antibody leveldifferences of different vaccine combinations on porcine epidemic diarrhea (PED), porcine transmissible gastroenteritis (TGE), porcine rotavirus (PoR)IgG, and PED IgA. The antibody level was detected by ELISA. Results showed that the antibody levels of PED and TGE IgG in serum and PED IgA inbreast milk of the “PT+PT*” vaccine combination group were higher than those of the other groups, and vaccine combination including “PTR” couldstimulate the sows to produce PoR IgG antibody. These findings revealed that the vaccine combination of “PT+PT*” is optimal for preventing porcineviral diarrhea, and “PTR+PT*” may be an alternative option in areas under PoRV infection risk. This study suggested that pig farms should selectsuitable immunization on the basis of the local epidemic situation of porcine viral diarrhea.

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1. Park SJ, Kim HK, Song DS, Moon HJ, Park BK: Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea. Arch Virol, 156 (4): 577-585, 2011. DOI: 10.1007/s00705- 010-0892-9
2. Li W, Li H, Liu Y, Pan Y, Deng F, Song Y, Tang X, He Q: New variants of porcine epidemic diarrhea virus, China, 2011. Emerg Infect Dis, 18 (8): 1350-1353, 2012. DOI: 10.3201/eid1808.120002
3. Lv C, Xiao Y, Li X, Tian K: Porcine epidemic diarrhea virus: Current insights. Virus Adapt Treat, 8, 1-12, 2016. DOI: 10.2147/vaat.s107275
4. Wang Y, Gao X, Yao Y, Zhang Y, Lv C, Sun Z, Wang Y, Jia X, Zhuang J, Xiao Y, Li X, Tian K: The dynamics of Chinese variant porcine epidemic diarrhea virus production in vero cells and intestines of 2-day old piglets. Virus Res, 208, 82-88, 2015. DOI: 10.1016/j.virusres.2015.06.009
5. Chen Q, Li G, Stasko J, Thomas JT, Stensland WR, Pillatzki AE, Gauger PC, Schwartz KJ, Madson D, Yoon KJ, Stevenson GW, Burrough ER, Harmon Km, Main RG, Zhang J: Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States. J Clin Microbiol, 52 (1): 234- 243, 2014. DOI: 10.1128/JCM.02820-13
6. Song D, Park B: Porcine epidemic diarrhoea virus: A comprehensive review of molecular epidemiology, diagnosis, and vaccines. Virus Genes, 44 (2):167-175, 2012. DOI: 10.1007/s11262-012-0713-1
7. Doyle LP, Hutchings LM: A transmissible gastroenteritis in pigs. J Am Vet Med Assoc, 108 (3): 257-259, 1946.
8. Hu XJ, Li NJ, Tian ZJ, Yin XJ, Qu L, Qu J: Molecular characterization and phylogenetic analysis of transmissible gastroenteritis virus HX strain isolated from China. BMC Vet Res, 11, 72, 2015. DOI: 10.1186/s12917-015- 0387-8
9. Miyazaki A, Kuga K, Suzuki T, Kohmotoe M, Katsuda K, Tsunemitsu H: Genetic diversity of group A rotaviruses associated with repeated outbreaks of diarrhea in a farrow-to-finish farm: Identification of a porcine rotavirus strain bearing a novel VP7 genotype, G26. Vet Res, 42, 112, 2011. DOI: 10.1186/1297-9716-42-112
10. Dang W, Fang L, Xiao S: Porcine epidemic diarrhea in China. Virus Res, 226, 7-13, 2016. DOI: 10.1016/j.virusres.2016.05.026
11. Yang DQ, Ge FF, Ju HB, Wang J, Liu J, Ning K, Liu PH, Zhou JP, Sun QY: Whole-genome analysis of porcine epidemic diarrhea virus (PEDV) from eastern China. Arch Virol, 159 (10): 2777-2785, 2014. DOI: 10.1007/ s00705-014-2102-7
12. Macpherson AJ, Mccoy KD, Johansen FE, Brandtzaeg P: The immune geography of IgA induction and function. Mucosal Immunol, 1, 11-22, 2008. DOI: 10.1038/mi.2007.6
13. Mantis NJ, Rol N, Corthésy B: Secretory IgA’s complex roles in immunity and mucosal homeostasis in the gut. Mucosal Immunol, 4, 603- 611, 2011. DOI: 10.1038/mi.2011.41
14. Langel SN, Paim FC, Lager KM, Vlasova AN, Saif LJ: Lactogenic immunity and vaccines for porcine epidemic diarrhea virus (PEDV): Historical and current concepts. Virus Res, 226, 93-107, 2016. DOI: 10.1016/ j.virusres.2016.05.016
15. Annamalai T, Saif LJ, Lu Z, Jung K: Age-dependent variation in innate immune responses to porcine epidemic diarrhea virus infection in suckling versus weaned pigs. Vet Immunol Immunopahol, 168 (3-4): 193- 202, 2015. DOI: 10.1016/j.vetimm.2015.09.006
16. Jung K, Saif LJ: Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis. Vet J, 204 (2): 134- 143, 2015. DOI: 10.1016/j.tvjl.2015.02.017
17. Hu XY, Zhang SX, Feng XF, Yue J, Wang KG, Wen M, Zhou BJ, Cheng ZT, Wang W, Qin WX: Growth and decline of PEDV IgA antibody in sow milk after immunization with porcine viral diarrhea vaccine. Chinese J Vet Sci, 37 (9): 1659-1663, 2017. DOI: 10.16303/j.cnki.1005-4545.2017.09.04
18. Chen F, Ku X, Li Z, Memon AM, Ye S, Zhu Y, Zhou C, Yao L, Meng X, He Q: Genetic characteristics of porcine epidemic diarrhea virus in Chinese mainland, revealing genetic markers of classical and variant virulent parental/attenuated strains. Gene, 588 (1): 95-102, 2016. DOI: 10.1016/j.gene.2016.05.011
19. Yu J, Chai X, Cheng Y, Xing G, Liao A, Du L, Wang Y, Lei J, Gu J, Zhou J: Molecular characteristics of the spike gene of porcine epidemic diarrhoea virus strains in Eastern China in 2016. Virus Res, 247, 47-54, 2018. DOI: 10.1016/j.virusres.2018.01.013
20. Lan DL: Molecular cloning and phylogenetic analysis of the E gene of transmissible gastroenteritis virus (TGEV) isolated in China. Afr J Microbiol Res, 6 (20): 907-916, 2012.
21. Yu TF, Li M, Shao SL, Lv JW, Xu XJ, Xu S: Genetic variation analysis of TGEV spike protein antigenic sites. J Anim Vet Adv, 11 (3): 361-363, 2012. DOI: 10.3923/javaa.2012.361.363
22. Molinari BLD, Possatti F, Lorenzetti E, Alfieri AF, Alfieri AA: Unusual outbreak of post-weaning porcine diarrhea caused by single and mixed infections of rotavirus groups A, B, C, and H. Vet Microbiol, 193, 125- 132, 2016. DOI: 10.1016/j.vetmic.2016.08.014
23. Vlasova AN, Amimo JO, Saif LJ: Porcine rotaviruses: Epidemiology, immune responses and control strategies. Viruses, 9 (3): 48, 2017. DOI: 10.3390/v9030048
24. Zhang Q, Hu R, Tang X, Wu C, He Q, Zhao Z, Chen H, Wu B: Occurrence and investigation of enteric viral infections in pigs with diarrhea in China. Arch Virol, 158 (8): 1631-1636, 2013. DOI: 10.1007/ s00705-013-1659-x
25. Liu Q: Establishment and application of IgG and IgA antibody deletion method for porcine epidemic diarrhea virus. BVSc, Hunan Agricultural University, Hunan, China, 2016.