Necdet Cankat LEHİMCİOĞLU, Yavuz ÖZTÜRKLER, Savaş YILDIZ, Umut Çağın ARI

The Effect of Intrauterine Infusion of Carvacrol After Insemination on Conception Rate in Repeat Breeder Cows Subjected to Progesteron Based Estrus Synchronization Protocol

Repeat breeder (RB) is one of the crucial causes of economic loss in dairy cows. The aim of this study was to determine the effects of intrauterine carvacrol administration after timed artificial insemination (TAI) on the conception rate (CR) in RB cows. The study involved 155 RB cows returned to service for more than three times and without any significant pathologic defects in genital tract. All cows were subjected to following estrus synchronization method: An intra-vaginal apparatus (IVA) containing 1.55 g progesteron were inserted for 9 days and administered one dose of PGF2α one day before removing IVA, then injected one dose of GnRH 6 h before TAI. All the cows were inseminated in 56th h after removing IVA. All RB cows were randomly divided to three groups. Group I (GI; n=52) and Group II (GII; n=51), treatments groups were infused with 0.1% diluted Carvacrol and 0.1% Lugol’s (GII) solution. Group III (GIII; n=51) the control group, was received normal saline only. All infusions were administered one dose of 30-50 mL (according to uterine size) into uterus half hour after insemination. In the observations performed in 21 to 30 days after insemination, non-return rates (NRR) were 71.15%, 69.23% and 68.62% in groups of GI, GII and GIII, respectively. CR detected by rectal palpation at 60 days following artificial insemination were 67.30%, 63.46% and 46.15% in GI, GII and GIII, respectively. However, the differences between groups were not statistically significant. As a result: In the treatment of RB cows, it may be suggested that carvacrol infusion to the uterus may be preferred as it has improved pregnancy rates to some extent. Nevertheless, further investigations are needed to confirm these results.

Progesteron Temelli Östrus Senkronizasyonu Protokolüne Tabi Tutulan Repeat Breeder İneklerde Suni Tohumlama Sonrası İntrauterin Carvacrol İnfüzyonunun Gebelik Oranları Üzerine Etkisi

Repeat Breeder (RB), süt ineklerinde ekonomik kaybın önemli nedenlerinden biridir. Bu çalışmanın amacı, RB ineklerinde zamanlanmış suni tohumlama sonrası intrauterin carvacrol uygulamasının gebelik oranlarına (GO) etkilerini belirlemektir. Çalışma, 3 defadan fazla geri dönen, genital sisteminde önemli patolojik bozukluk saptanmayan 155 adet RB inekte yürütüldü. Tüm inekler aşağıdaki östrus senkronizasyonu yöntemine tabi tutuldu: 1.55 g progesteron içeren bir intra-vajinal aparat (IVA), 9 gün boyunca yerleştirildi ve IVA çıkarılmasından bir gün önce bir doz PGF2α uygulandı ve suni tohumlamadan 6 saat önce bir doz GnRH enjekte edildi. Tüm inekler, IVA çıkarıldıktan 56 saat sonra tohumlandı. Tüm hayvanlar rastgele üç gruba ayrıldı. Uygulama grupları olan grup I (GI; n=52) ve grup II (GII; n=51)’ye sırasıyla %0.1 seyreltilmiş Carvacrol ve %0.1 Lugol (GII) çözeltileri verildi. Kontrol grubu grup III’e (GIII; n=51) sadece normal serum fizyolojik infüze edildi. Tüm infüzyonlar, tohumlamadan yarım saat sonra uterus içine bir doz 30-50 mL (uterus boyutuna göre) olacak şekilde uygulandı. Tohumlamadan 21 ila 30 gün sonra yapılan gözlemlerde, geri dönmeyenlerin oranı (NRR), GI, GII ve GIII gruplarında sırasıyla %71.15, %69.23 ve %68.62 olarak belirlendi. Suni tohumlamayı takiben 60. günde yapılan rektal palpasyonla teşhis edilen GO ise GI, GII ve GIII’de sırasıyla %67.30, %63.46 ve %46.15 olarak saptandı. Ancak, gruplar arasındaki farklar istatistiksel olarak anlamlı değildi. Sonuç olarak: RB ineklerin tedavisinde, uterusa carvacrol infüzyonunun gebelik oranlarını bir dereceye kadar arttırdığı için tercih edilebileceği kanaatine varıldı. Fakat, bu sonuçları pekiştirmek için başka araştırmalara ihtiyaç olduğu düşünülmektedir.

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