Ahmet ÜNVER, MEHMET ÇİTİL, Halil İ. ATABAY, SALİH OTLU, MİTAT ŞAHİN

Yenidoğan buzağı ishallerinden Salmonella ve Citrobacter türlerinin izolasyon ve identifikasyonu

Bu çalışma, Salmonella ve Citrobacter türlerinin yenidoğan ishalli buzağılardaki prevalansını ortaya koymayı amaçlamıştır. Kars yöresinde yetiştirilmekte olan toplam 155 adet yenidoğan ishalli buzağıdan alınan dışkı örneklerinin 3 tanesinden Salmonella spp., farklı 3 tanesinden de Citrobacter spp. izole ve identifiye edildi. Bu sonuçlara göre hem Salmonella hem de Citrobacter türlerinin ishalli yenidoğan buzağılardaki prevalansı % 1.93 olarak bulundu. Yörede ishal vakalarında Salmonella türlerinin önemini koruduğunu ve Citrobacter türlerinin yenidoğan buzağılarda ishal nedeni olabileceğini ortaya koyan bu çalışma, bu etkenlerin ishal vakalarının teşhisinde, tedavisinde, kontrol ve koruma çalışmalarında dikkate alınması gerekliliğini ortaya koymuştur.

Anahtar Kelimeler:

Citrobacter, buzağı, yenidoğan, Salmonella, ishal

Isolation and identification of Salmonella spp. and Citrobacter spp. from diarrheic neonatal calves

The purpose of this study was to determine the prevalence of Salmonella spp. and Citrobacter spp. in diarrheic neonatal calves. In 155 fecal samples analyzed for presence of these agents, Salmonella spp. and Citrobacter spp. were isolated and identified from 3 different samples. These results showed that prevalence of these microorganisms was 1.93% in diarrheic neonatal calves. The current study presents the significance of possible Salmonella- and Citrobacter-related diarrhoea, therefore, these agents need to be considered for diagnosis, prophylaxis, control and prevention of diarrhoea of neonatal calves in Kars region.

Keywords:

Citrobacter, calves, neonates, Salmonella, diarrhoea,

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1.Acres SD, Laing CJ, Saunders JR, Radostits OM: Acute undifferentiated neonatal diarrhea in beef calves. I. Occurrence and distribution of infectious agents. Can J Comp Med, 39(2): 116-132, 1975.
2.Arda M: Neonatal buzağılarda ishaller ve neonatal bağışıklık. Etlik Vet Mikrobiyol Derg, 6(2): 143-166, 1988.
3.Arda M, Aydın N, İlgaz A, Minbay A, Kahraman M, İzgür M, Leloğlu N, Akay Ö, Diker KS: Özel mikrobiyoloji. 5.baskı. Medisan, Ankara, 1999.
4.Carter GR, Chengappa MM: Essentials of Veterinary Microbiology and Mycology. 4th Ed. Lea and Febiger, 1991.
5.Ball HJ, Finlay D, Zafar A, Wilson T: The detection of verocytotoxins in bacterial cultures from human diarrhoeal samples with monoclonal antibody-based ELISAs. Med Microbiol, 44(4):273-6, 1996.
6.Bellinzoni RC, Blackball J, Terzolo HR, Moreira AR, Auza N, Mattion N, Micheo GL, La Torre JL, Scodeller EA: Microbiology of diarrhoea in young beef and dairy calves in Argentina. Rev Argent Microbiol, 22(3): 130-136, 1990.
7.Schmidt H, Montag M, Bockemuhl J, Heesemann J, Karch H: Shiga-like toxin II-related cytotoxins in Citrobacter freundii strains from humans and beef samples. Infect Immun, 61(2):534-543, 1993.
8.Aydin F, Umur Ş, Gökçe G, Genç O, Güler MA: Kars yöresindeki ishalli buzağılarda bakteriyel ve paraziter etkenlerin izolasyonu ve identifikasyonu. Kafkas Üniv Vet Fak Derg, 7(1) 7-14. 2001.
9 Bulgin MS, Anderson BC, Ward AC, Evermann JF: Infectious agents associated with neonatal calf disease in southwestern Idaho and eastern Oregon. J Am Vet Med Assoc, 180(10): 1222-1226, 1982.
10.Hall GA, Reynolds DJ, Parsons KR, Bland AP, Morgan JH: Pathology of calves with diarrhoea in southern Britain. Res VetSci, 45 (2): 240-250, 1988.
11.Reynolds DJ, Morgan JH, Chanter N, Jones PW, Bridger JC, Debney TG, Bunch KJ: Microbiology of calf diarrhoea in southern Britain. VetRec, 119(2):34-39, 1986.
12.Salyers A, Dixie A, Whitt D: Bacterial Pathogenesis: a Molecular Approach, Second Edition, Washington, D.C., USA, ASM Press, 1994
13.Guarino A, Capano G, Malamisura B, Alessio M, Guandalini S, Rubino A: Production of Escherichia coli STa- like heat-stable enterotoxin by Citrobacter freundii isolated from humans. J Clin Microbiol, 25(1): 110-114, 1987.