Emine ATAKİŞİ, MAHMUT KARAPEHLİVAN, ONUR ATAKİŞİ, AYLA ÖZCAN, MEHMET ÇİTİL

Fenilhidrazin verilen farelerde L-karnitinin karaciğer dokusundaki koruyucu etkisinin araştırılması

Çalışmada fenilhidraziri ile farelerde oluşturulan karaciğer hasarında L-karnitinin koruyucu etkisinin araştırılması amaçlandı. Bu amaçla Swiss albino cinsi fareler, kontrol, fenilhidrazin (40 mg/kg/fare), fenilhidrazin + L-karnitin (40 mg/kg/fare ve 100 mg/kg/fare/gün) ve L-karnitin (100 mg/kg/fare/gün) olmak üzere dört eşit gruba ayrıldı. Periton içi enjeksiyonlar günlük olarak 7 gün boyunca yapıldı. Yedinci günde fareler ötenazi edildi. Karaciğer dokuları toplandı. AST, ALT aktiviteleri ile üre, total protein, kreatinin, demir, total sialik asit (TSA) ve glukoz konsantrasyonları ölçüldü. Fenilhidrazin grubunda karaciğer AST ve ALT aktiviteleri kontrol grubuna göre anlamlı derecede düşük (p

Anahtar Kelimeler:

karaciğer, fareler, karnitin

Investigation of protective effect of L-carnitine in the liver tissue of phenylhydrazine given mice

Aim of this study was to investigate the effects of L-carnitine in phenylhydrazine-induced liver damage in mice. For that purpose, twenty-eight Swiss albino mice were divided into four equal groups as follows: Control, 0.5 ml/day 0.9% NaCl, phenylhydrazine (40 mg/kg/day per mouse), phenylhydrazine plus L-carnitine (40 mg/kg phenylhydrazine and 1000 mg/kg L-carnitine per day per mouse) and L-carnitine group (1000 mg/kg per day per mouse). Intraperitoneal injections were carried out daily for 7 days. On day 7, mice were euthanasied and sacrificed, liver tissues were collected and activities of AST, ALT, urea, total protein, creatinin, iron, total sialic acid (TSA) and glucose were determined. Compared to control group, liver AST and ALT activities were significantly lower but urea, total protein, creatinin, iron, TSA and glucose were higher in phenylhydrazine group. However, there was no statistical difference between control group and phenylhydrazine plus L-carnitine group in terms of the status of the parameters measured except urea levels. In conclusion, the present study suggests that L-carnitine possesses protective roles in phenylhydrazine-induced liver damage in mice, possibly by protecting cell membranes of hepatocytes and by binding iron and toxic substances

Keywords:

liver, mice, carnitine,

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