İDRİS TÜREL, ALİ ERTEKİN, GÖKHAN OTO, F. Çağlar ÇELİKEZEN, SEMİH YAŞAR

Urtica dioica L. (ısırgan otu)'nin metanol ve su ekstraktının 7.12-dimetilbenz(a)antrasen uygulanan tavşan tüylerindeki iz element seviyeleri üzerine etkileri

Bu çalışmada, Urtica dioica L.’nin, bir karsinojenik madde olan polisiklik aromatik hidrokarbonlardan 7,12- dimetilbenz(a)antrasen (DMBA) uygulanan tavşanların tüylerindeki iz element (Zn, Cu, Mn, Fe) konsantrasyonlarına etkileri araştırıldı. Her biri yedişer tavşandan oluşan üç deneme ve bir kontrol grubu oluşturuldu. A grubuna 7,12- dimetilbenz(a)antrasen (0,5 ml/kg/gün), B grubuna 0,5 ml/kg/gün DMBA+0.2 ml/kg/gün dozunda ısırgan otu metanol ekstraktı, C grubuna 0,5 ml/kg/gün DMBA+0.5 ml/kg/gün dozunda ısırgan otu sulu ekstraktı ve kontrol grubuna ise fizyolojik tuzlu su ile hazırlanan %10’luk dimetilsülfoksit çözeltisinden 0.5 ml/kg/gün dozunda İM olarak beş ay süreyle uygulandı. Yapılan ölçümlerde Cu düzeylerinde gözlenen azalmalar kontrol grubu ile karşılaştırıldığında istatistik olarak anlamlı bulundu (P

Anahtar Kelimeler:

kürk, Urtica dioica, eser elementler, tavşan, bitki özütü, metanol, tıbbi bitkiler, karsinojenler

The effects of nettle herb (Urtica dioica L.) on the levels of trace elements in the rabbit fur treates with 7.12-dimetylbenzanthracene

In this study the effect of nettle herb (Urtica dioica L.) on the levels of trace element (Zn, Cu, Mn, Fe) in the rabbit fur treated with 7,12-dimetylbenzanthracene (DMBA) were investigated. The rabbit were divided into three experimental groups and one control group, each group contained seven rabbits. DMBA (0.5 ml/kg/day) were administreated IM to group A as carcinogenic compound. DMBA (0.5 ml/kg/day) were administreated IM as carcinogenic compound plus methanol extract of nettle herb (0.2 ml/kg/day) to group B, DMBA (0.5 ml/kg/day) were administreated IM as carcinogenic compound plus water extract of nettle herb (0.5 ml/kg/day) to group C and 10% dimethylsulphoxide (0.5 ml/kg/day) dissolved in normal saline solution were administrated to control group. All of the treatments were performed for five mounths to each group. The decrases in Cu levels were significant when compared with that of control group (P<0.01). The decrases in Fe levels in water extract of DMBA+Urtica dioica L. was statistically significant compared with that of control group (P<0.05). In conclusion, fur trace element levels of groups treated with methanol or water extract of Urtica dioica L. were less compared with that of control group but were higher compared with that of DMBA group. It was thought that Urtica dioica L. can have protective effect against damage caused by DMBA.

Keywords:

fur, Urtica dioica, trace elements, rabbits, plant extracts, methanol, medicinal plants, carcinogens,

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