OKTAY ÖZKAN, Handan AYDIN, A. Funda BAĞCIGİL

Salvia verticillata ve Phlomis pungens'in vitro antibakteriyel etkinliğinin değerlendirilmesi

Bu çalışmada Salvia verticillata L. subsp. amasiaca (Freyn & Bornm) Bornm. ve Phlomis pungens Willd. var. hirta Velen’in yaprak ve çiçek metanolik ekstraktlarının antimikrobiyal aktiviteleri test edilmiştir. Metanolik ekstraktların 9 farklı bakteri türüne (Klebsiella pneumoniae ATCC 4352, Pseudomonas aeruginosa ATCC 27853, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 12228, Bacillus cereus ATCC 11778, Salmonella enteritidis KUEN 349, Proteus mirabilis CCM1944, Escherichia coli ATCC 25922) karşı antibakteriyel etkileri macro broth dilüsyon yöntemi kullanılarak tespit edildi. Salvia verticillata yaprak ve çiçek methaol extraktları Pseudomonas aeruginosa, Salmonella enteritidis, Escherichia coli, Bacillus cereus, Bacillus subtilis ve Staphylococcus aureus’a karşı belirgin bir etkinlik gösterirken Phlomis pungens yaprak ve çiçek ekstraktları Pseudomonas aeruginosa ve Bacillus subtilis’e karşı etkili olmuşlardır. Sonuç olarak bu iki bitki türünün çeşitli enfeksiyöz hastalıklarda antmikrobiyallere alternatif olarak kullanılabileceğini düşünmekteyiz.

Anahtar Kelimeler:

antimikrobiyal özellikler, çiçekler, yapraklar, tıbbi bitkiler, metanol, bitki özütü

In vitro evaluation of antimicrobial activities of salvia verticillata and Phlomis pungens

This study, the methanol extracts of Salvia verticillata L. subsp. amasiaca (Freyn & Bornm) Bornm. and Phlomis pungens Willd. var. hirta Velen from flowers and leaves have been studied for antimicrobial activity. Antibacterial activity of the methanol extracts was determined against 9 bacterial strains (Klebsiella pneumoniae ATCC 4352, Pseudomonas aeroginosa ATCC 27853, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 12228, Bacillus cereus ATCC 11778, Salmonella enteritidis KUEN 349, Proteus mirabilis CCM1944, Escherichia coli ATCC 25922) using the macro broth dilution method. The methanol extract of the leaves and flowers of Salvia verticillata remarkably inhibited the growth of Pseudomonas aeruginosa, Salmonella enteritidis, Escherichia coli, Bacillus cereus, Bacillus subtilis and Staphylococcus aureus. On the other hand, the results indicate that the methanol extract of the leaves and flowers of Phlomis pungens remarkably inhibited the growth of Pseudomonas aeruginosa and Bacillus subtilis. In conclusion, the results show that Salvia verticillata and Phlomis pungens methanol extracts might be an alternative to antimicrobials in various infectious diseases.

Keywords:

antimicrobial properties, flowers, leaves, medicinal plants, methanol, plant extracts,

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