İSMAİL SEVEN, PINAR TATLI SEVEN, Seval YILMAZ

Responses of broilers under cold conditioning $(15^oC)$ to dietary triiodothyronine and lodine combined to antioxidants (Selenium and Vitamin C)

Bu araştırma soğuğa maruz kalan broylerlerde diyetsel antioksidanların (selenyum (Se) ve vitamin C) ve triiodotiroidinin (T3) biyokimyasal parametreler, malondialdehit (MDA) ve katalaz (CAT) üzerine etkilerini belirlemek için yapıldı. Çalışmada bir günlük yaşta 150 adet broyler civcivler (Ross 308) kullanıldı. Civcivler her biri 30 civcivden oluşan 1 kontrol ve 4 deneme grubuna tesadüfi olarak ayrıldı ve her deneme grubu 10 civcivden oluşan 3 tekerrür grubu ihtiva etti. Deneme grupları şöyledir; kontrol grubuna bazal diyet yedirildi, grup 1’e sodyum selenit olarak 1 mg/kg selenyum + kalsiyum iyodat olarak 2 mg/kg iyot katkılı basal diyet yedirildi; grup II’ye 1 mg/kg selenyum + 1 mg/kg T3 katkılı bazal diyet yedirildi; grup III’e askorbik asit olarak 250 mg/kg vitamin C + 1 mg/kg iyot katkılı bazal diyet yedirildi; grup IV’e askorbik asit olarak 250 mg/kg vitamin C + 1 mg/kg T3 katkılı bazal diyet yedirildi. Plazma T3, trigliserit ve SGPT gruplar arasında önemli oranda farklıydı. Kontrol grubunun kalp dokusundaki MDA düzeyi (P

Anahtar Kelimeler:

askorbik asit, antioksidanlar, etlik piliç, soğuk, soğuk stresi, soğuk toleransı, diyet, iyot, kümes hayvanları beslenmesi, selenyum, triiyodotironin

Soğuk koşullar altındaki $(15^oC)$ broylerlerin diyetsel antioksidanlarla (Selenyum ve Vitamin C) kombine edilen triiodotiroidinin ve iyodine tepkileri

This experiment was conducted to determine the influence of dietary antioxidants (selenium (Se) and vitamin C), and triiodothyronine (T3) to iodine on biochemical parameters, malondialdehyde (MDA), catalase (CAT) in broilers exposed cold. In the study, 150, one-day-old, broiler chicks (Ross 308) were used. Chicks were randomized into 1 control and 4 treated groups each containing 30 birds and each experimental group comprised 3 replicates of 10 birds. The experimental groups were as follow: control was fed with basal diet; group I was fed with basal diet supplemented with 1 mg/kg selenium as sodium selenite plus 2 mg/kg iodine as calcium iodate; group II was fed with basal diet supplemented 1 mg/kg selenium plus 1 mg/kg T3; group III was fed with basal diet supplemented with 250 mg/kg vitamin C as ascorbic acid plus 1 mg/kg iodine; group IV was fed with basal diet supplemented with 250 mg/kg vitamin C plus 1 mg/kg T3. Plasma T3, triglyceride and SGPT were significantly different among the groups. MDA level in heart tissue of control group was found significantly higher than those of other groups (P<0.01). MDA levels in liver (P<0.01) and abdominal fat (P<0.001) tissues of control and group I were determined significantly higher than those of other groups, whereas MDA level in lung tissue was similar found in all groups. The CAT activity of liver of control and group I was found significantly the highest (P<0.001). Results showed that cold exposure in broilers induced oxidative damage in tissues, but this damage decreased partly in supplement groups, except group I.

Keywords:

ascorbic acid, antioxidants, broilers, cold, cold stress, cold tolerance, diet, iodine, poultry feeding, selenium, triiodothyronine,

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1. Filizciler M, Cerci IH, Tatli P: Sıcak stresi altındaki SPF (Specific Pathogen Free) beyaz yumurtacı tavuklarda gece yemlemesinin etkileri. Turk J Vet Anim Sci, 26, 439-446,2002.
2. Cerci IH, Tatli P, Azman MA, Birben N: The effect of restricted feed on feed intake, egg production and feed conversion in pullets. Indian Vet J, 80, 1153-1157, 2003.
3. Aksu T, Bozkurt AS: Effect of dietary essential oils and/or humic acids on broiler performance, microbial population of intestinal content and antibody titres in the summer season. Kafkas Univ Vet Fak Derg, 15, 185-190, 2009.
4. Venditti P, De Rosa R, Portero-Otin M, Pamplona R, Di Meo S: Cold-induced hyperthyroidism produces oxidative damage in rat tissues and increases susceptibility to oxidants. Int J Biochem Cell B, 36, 1319-1331, 2004.
5. Sewerynek E, Wiktorska J, Lewinski A: Effects of melatonin on the oxidative stress induced by thyrotoxicosis in rats. Neuro Endocrinol Lett, 20, 157-161, 1999.
6. Tatli Seven P, Seven I, Yilmaz M, Simsek G: The effects of Turkish propolis on growth and carcass characteristics in broilers under heat stress. Anim Feed Sci Technol, 146, 137- 148, 2008.
7. Gerloff BJ: Effect of Se supplementation on dairy cattle. J Anim Sci, 70, 3934-3940, 1992.
8. Tatli Seven P, Yilmaz S, Seven I, Dalkilic B: Responses of broilers to triiodothyronine hormone and iodine supplements in cold environment (15ºC). Indian Vet J, 86, 566-569, 2009.
9. Anonymus: http://vitamins.ygoy.com/2007/10/20/thyroidand- vitamins/#nn, 2007.
10. Tatli Seven P: The Effects of dietary Turkish propolis and vitamin C on performance, digestibility, egg production and egg quality in laying hens under different environmental temperatures. Asian-Aust J Anim Sci, 21, 1164-1170, 2008.
11. Tatli Seven P, Yilmaz S, Seven I, Cerci IH, Azman MA, Yilmaz M: The effect of propolis on some blood parameters and antioxidant enzyme activities in broilers under heat stres. Acta Vet Brno, 78, 75-83, 2009.
12. National Research Council (NRC): National Academy Press. Washington, USA, 1994.
13. Brown MW, Watkinson JH: An automated fluorimetric method for the determination of nanogram quantities of selenium. Anal Chim Acta, 89, 29-35, 1977.
14. Groppel B: Jodmangelerscheinungen, Jodversorgung und Jodstatus des Wiederkauers (Rind, Schaf, Ziege). Promotion B, Wissenschaftliche Rat der Karl-Marx-Universitat, Leipzig,1987.
15. Satoh K: Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta, 90, 37-43, 1978.
16. Ohkawa H, Ohishi N, Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-358, 1979.
17. Aebi H: Catalase. Methods of enzymatic analysis. 673- 678. Academic Press, New York, USA, 1974
18. Ergün G, Aktaş S: ANOVA modellerinde kareler toplamı yöntemlerinin karşılaştırılması. Kafkas Univ Vet Fak Derg, 15, 481-484, 2009.
19. SPSS: Base System User’s Guide, Release10.1, for Windows SPSS inc. Chicago, USA, 1999.
20. Petrovic N, Cvijic G, Davidovic V: Thyroxine and triiodothyronine differently affect uncoupling protein-1 content and antioxidant enzyme activities in rat interscapular brown adipose tissue. J Endocrinol, 176, 31-38, 2003.
21. Tatli Seven P, Seven I, Yılmaz S, Dalkilic B: The effects of selenium and vitamin C supplementation on lipid peroxidation in broilers reared cold environment (15ºC) and diets of high energy. Fırat Univ Sağ Bil Derg, 23, 15-19, 2009.
22. Tona K, Onagbesan OM, Bruggeman V, Mertens K, Jego Y, Decuypere E: Comparison of feed intake, blood metabolic parameters, body and organ weights of growing broilers originating from dwarf and standard broiler breeder lines. Int J Poult Sci, 3, 422-426, 2004.
23. Aceves C, Anguiano B, Delgado G: Is iodine a gatekeeper of the integrity of the mammary gland? J Mammary Gland Biol, 10, 189-196, 2005.
24. Yuming G: The necessity of selenium to the normal thyroid hormone metabolism of rats. PhD dissertation, Beijing Agricultural University, China, 1991.
25. Martinello F, Soares SM, Franco JJ, Santos AC, Sugohara A, Garcia SB, Curti C, Uyemura SA: Hypolipemic and antioxidant activities from Tamarindus indica L. pulp fruit extract in hypercholesterolemic hamsters. Food Chem Toxicol, 44, 810-818, 2006.
26. Mercan U: Toksikolojide serbest radikallerin önemi. YYU Vet Fak Derg, 15, 91-96, 2004.
27. Ruhl CE, Everhart JE: Relation of elevated serum alanine aminotransferase activity with iron and antioxidant levels in the United States. Gastroenterology, 124, 1821-1829, 2003.
28. Kaushic S, Kaur J: Chronic cold exposure affects the antioxidant defense system in various rat tissues. Clin Chim Acta, 333, 69-77, 2003.
29. Mujahid A, Furuse M: Oxidative damage in different tissues of neonatal chicks environmental temperature. Comp Biochem Phys A, 152, 604-608, 2009.
30. Jianhua H, Ohtsuka A, Hayashi K: Selenium influences via thyroid hormone status in broiler chickens. Brit J Nutr, 84, 727-732, 2000.
31. Zamoner A, Barreto KP, Filho DW, Sell F, Woehl VM, Rodrigues Guma FC, Barreto Silva FRM, Pessoa-Pureur R: Hyperthyroidism in the developing rat testis is associated with oxidative stress and hyperphosphorylated vimentin accumulation. Mol Cell Endocrinol, 267, 116-126, 2007.
32. Huston TM, Carmon JL: The influence of high environmental temperature on thyroid size of domestic fowl. Poultry Sci, 41, 175-183, 1962.
33. Nayyar H, Bains TS, Kumar S: Chilling stressed chickpea seedlings: effect of cold acclimation, calcium and abscisic acid on cryoprotective solutes and oxidative damage. Environ Exp Bot, 54, 275-285, 2005.
34. Buzadzic B, Korac B, Petrovic VM: The effect of adaptation to cold and re-adaptation to room temperature on the level of glutathione in rat tissues. J Therm Biol, 24, 373- 377, 1999.