Türkiye’de yetiştirilen altı yerli koyun ırkında BMPR-IB (Booroola) geninde FecB allel varlığının PCR-RFLP yöntemiyle araştırılması

Bu çalışmada, Türkiye’de yetiştirilen altı yerli koyun ırkında, çoklu doğumu arttıran Booroola (BMPR-IB) majör geninde FecB allelinin varlığı PCR-RFLP metodu kullanılarak araştırılmıştır. Yapılan analizler sonucunda Türkiye’nin değişik bölgelerinde yetiştirilen sürülerden rastgele seçilen Akkaraman (19 örnek), Morkaraman (19 örnek), Dağlıç (18 örnek), İvesi (18 örnek), Tuj (18 örnek) ve Karakaş (19 örnek) koyunlarına ait toplam 111 örnekte FecB alleli tespit edilememiştir.

Anahtar Kelimeler:

analitik yöntemler, teknikler, koyun ırkları, koyun, kısıtlı fragment uzunluğu polimorfizmi, üreme, polimeraz zincir reaksiyonu, genler, döllenme gücü, alleller, Türkiye, Tuj

An investigation of presence of FecB allele on BMPR-IB (Booroola) gene raised in Turkey in six local sheep breeds using PCR-RFLP method

The presence of FecB allele on Booroola major gene (BMPR-IB) which has an significant effect on the prolificacy was investigated in six local sheep breeds reared in Turkey, by using PCR-RFLP method. As a result of these analysis, FecB allele couldn’t be determined in totally 111 samples obtained from Akkaraman (19 samples), Morkaraman (19 samples), Dağlıç (18 samples), Ivesi (18 samples), Tuj (18 samples) and Karakaş (19 samples), which are the sheeps randomly selected from the flocks which are reared in different regions of Turkey.

Keywords:

analytical methods, techniques, sheep breeds, sheep, restriction fragment length polymorphism, reproduction, polymerase chain reaction, genes, fecundity, alleles, Turkey, Tuj,

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1. Laçin E, Aksoy AR: Kars bölgesinde yetiştirilen Morkaraman ve Tuj kuzularının büyüme özelliklerinin karşılaştırılması. Kafkas Univ Vet Fak Derg, 9 (1): 33-37, 2003.
2. Karaca O, Cemal İ, Atay O: Hayvancılıkta kimi majör genlerin aktarımı ve kullanımı olanakları. Ulusal Hayvancılık Kongresi, İzmir, 728-732, 18-20 Eylül, 1996.
3. Morris CA: Theoretical and realised responses to selection for reproductive rate. Proc of the 4th World Congress on Genetics Applied to Livestock Production, Edinburgh, 16, 309-318, July, 23-27, 1990.
4. Montogomery GW, Mcnatty KP, Davis GH: Physiology and molecular genetics of mutations that increase ovulation rate in sheep. Endocr Rev, 13 (2): 309-328, 1992.
5. Cemal İ: Çiftlik hayvanlarında majör genler, bunların belirlenmesi, transferi ve endüstriyel kullanımı. Yüksek lisans tezi. Yüzüncü Yıl Üniv Fen Bil Enst, Van, 1996.
6. Davis GH: Fecundity genes in sheep. Anim Reprod Sci, 82- 83, 247-253, 2004.
7. Wilson T, Wu XY, Juengel JL, Ross IK, Lumsden M, Lord EA, Dodds KG, Walling GA, McEwan JC, O’connel AR, Mcnatty KP, Montogomery GW: Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulosa cells. Biol Reprod, 64, 1225-1235, 2001.
8. Souza CJH, Macdougall C, Campbell BK, Mcneilly AS, Baird DT: The booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type IB (BMPRIB) gene. J Endocrinol, 169 (2): 1-6, 2001.
9. Mulsant P, Lecerf F, Fabre S, Schibler L, Monget P, Lenneluc I, Pisselet C, Riquet J, Monniaux D, Calebaut I, Cribıu E, Thimonieri J, Teyssieri J, Bodin L, Cognie Y, Chitour N, Elsen JM: Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes. Proc Natl Acad Sci, 98, 5104-5109, 2001.
10. Kumar S, Kolte AP, Mishra AK, Arora AL, Singh VK: Identification of the FecB mutation in Garole Malpura sheep and its effect on litter size. Small Rumin Res, 64, 305-310, 2006.
11. Hanrahan JP, Gregan MS, Mulsant P, Mullen M, Davis GH, Powel R, Galloway SM: Mutation in the genes for oocyte derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biol Reprod, 70, 900-909, 2004.
12. Davis GH, Balakrishnan L, Ross IK, Wilson T, Galloway SM, Lumsden JM, Hanrahan JP, Mullen M, Mao XZ, Wang GL, Zhao ZS, Zeng YQ, Robinson JJ, Mayrogenis AP, Papachristoforou C, Peter C, Baumung R, Cardyn P, Boujenane I, Cockett NE, Eythorsdottir E, Arranz JJ, Noter DR: Investigation of the Booroola (FecB) and Inverdale (FecXI) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries. Anim Reprod Sci, 92, 87–96. 2006.
13. Akyüz B, Ertuğrul O, Ağaoğlu ÖK: Detection of Bovine Leukocyte Adhesion Deficiency (BLAD) allele in Holstein cows reared in Kayseri vicinity. Kafkas Univ Vet Fak Derg, 16 (2): 519-521, 2010.
14. Tajangookeh HD, Shahneh AZ, Zamiri MJ, Daliri M, Kohram H, Javaremi AN: Study of BMP-15 gene polymorphism in Iranian goats. Afr J Biotechnol, 8 (13): 2929-2932, 2009.
15. Birben E: Polimeraz Zincir Reaksiyonu - Restriksiyon Parça Uzunluğu Polimorfizmi (PCR-RFLP) DNA’nın restriksiyon endonükleazlar ile kesimi. Astım Allerji İmmünoloji, 5 (1): 53- 55, 2007.
16. Miller S, Dykes D, Plesky HA: Simple salting out procedure for extracting DNA from human cells. Nucleic Acids Res, 16, 1215, 1988.
17. Davis GH., Galloway SM, Ross IK, Gregan SM, Ward J, Nimbkar BV, Ghalsasi PM, Nimbkar C, Gray DG, Inounu I, Tiesnamurti B, Martyniuk E, Eythorsdottir E, Mulsant P, Lecerf F, Hanrahan JP, Bradford GH, Wilson T: DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation. Biol Reprod, 66, 1869-1874, 2002.
18. Kolte AP, Mishra AK, Kumar S, Arora AL, Singh VK: A study on effect carrying FecB gene on body weight in Garole and GaroleXMalpura sheep. Asian-Aust J Anim Sci, 18 (10): 1379-1382, 2005.
19. Chu MX, Liu ZH, Jiao CL, He YQ, Fang L, Ye, SC, Chen GH, Wang JY: Mutation in BMPR-IB and BMP-15 genes are associated with litter size in small tailed Han sheep (Ovis aries). J Anim Sci, 85, 598-603, 2007.
20. Polat YO: Sakız koyun ırkında BMPR-IB geninde çoklu doğuma neden olabilecek FecB alleli varlığının PCR-RFLP yöntemi ile araştırılması. Doktora tezi. Uludağ Üniv Sağlık Bil Enst, Bursa, 2006.
21. Guan F, Liu, SR, Shi GQ, Yang LG: Polymorphism of FecB gene in nine sheep breeds or strains and its effects on litter size, lamb growth and development. Anim Reprod Sci, 99, 44-52, 2007.
22. Kasariyan MM, Hafezeyan H, Sayahzadeh H, Jamshidi R, Asghari SR, Irajeyan GH, Buesagh H: Genetic polymorphism FecB and BMP15 genes and its association with litter size in Sangsari sheep breed of Iran. J Anim Vet Adv, 8 (5): 1025- 1031, 2009.