Anton RUSENOV, Lazarin LAZAROV, Zvezdelina KIRKOVA, Anton TONEV, Nikolina RUSENOVA, Francesca DILDA

Transient glomerular dysfunction in dogs caused by dirofilaria immitis infection

Bu çalışmanın amacı sağlıklı ve Dirofilaria immitis ile enfekte köpeklerde ve ivermectin ve doxcycline ile tedavi sonrasında iki glomerüler markırdaki (üriner albumin, uALB; üriner C-reaktif protein, uCRP) muhtemel değişimleri araştırmaktır. Çalışmada Knott metodu and SNAP 4Dx (IDEXX, USA) testleriyle D. immitis ile enfekte olduğu tespit edilen yirmi köpek ile birlikte kontrol olarak aynı yaş grubunda klinik olarak sağlıklı 12 adet köpek kullanıldı. Kontrol grubuyla (uAlB/Cr mg/g: 94.44±56.50; uCRP/Cr mg/g: tespit limitinin altı) karşılaştırıldığında kalp kurdu olan köpeklerin glomerüler biomarkırlarında (ortalama, Standart sapma) (uAlB/Kreatin, Cr mg/g: 527.57±312.54; uCRP/Cr mg/g: 0.520±0.624) artma olduğu belirendi. İlk bakıdan sekiz ay sonra ve takip eden tedavi sonrasında tüm glomerüler markırların dikkati çeken derecede azaldığı ve sağlıklı olan hayvanların değerlerinden farklılık göstermediği belirlendi. Sonuç olarak, biomarkır değerlerinde gözlenen değişikliklerin köpeklerde kalp kurdu enfeksiyonu nedenli glomerüler disfonksiyonun değişken doğasını açıkça ortaya koyduğu ve bu durumun klinik değeri olabileceği gösterilmiştir.

Köpeklerde dirofilaria immitis enfeksiyonu nedenli transient glomerüler disfonksiyon

The present study aimed to examine two glomerular markers (urinary albumin, uALB; urinary C-reactive protein, uCRP) in healthy dogs and in dogs infected with Dirofilaria immitis, and to identify some possible changes in these markers after therapy with ivermectin and doxycycline. Twenty dogs with D. immitis infection positive by both the Knott method and SNAP 4Dx (IDEXX, USA) test were included in the research, as well as twelve clinically healthy dogs of similar age which served as controls. Glomerular biomarkers (meаn, SD) increased significantly in dogs with heartworm disease (uAlB/Creatinine, Cr mg/g: 527.57±312.54; uCRP/Cr mg/g: 0.520±0.624), compared to control dogs (uAlB/Cr mg/g: 94.44±56.50; uCRP/Cr mg/g: below detection limit). Six months after the initial examination and the simultaneous treatment, all glomerular markers were considerably decreased and did not difer from those in healthy animals. In conclusion, the observed changes in glomerular biomarkers clearly indicated the transient nature of glomerular dysfunction caused by the heartworm infection in dogs, which may be of clinical relevance.

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1. Ceribasi AO, Simsek S: Histopathologic efects of Dirofilaria immitis microfilaria on internal organs of dog confirming by PCR technique. Iran J Parasitol, 7, 103-107, 2012.
2. Ludders JW, Grauer GF, Dubielzig RR, Ribble GA, Wilson JW: Renal microcirculatory and correlated histologic changes associated with dirofilariasis in dogs. Am J Vet Res, 49, 826-830, 1988.
3. Chew D, DiBartola S: Diagnosis and pathophysiology of renal disease. In, Ettinger, SJ (Ed). Textbook of V eterinary I nternal M edicine. 3 rd ed., 1893-1961, Philadelphia, WB Saunders, 1989.
4. Finco DR: Kidney function. In, Kaneko JJ, Harvey JW, Bruss ML (Eds): Clinical B iochemistry of D omestic Animals. 441-484, San Diego, CA Academic Press, 1995.
5. Clemo FAS: Urinary enzyme evaluation of nephrotoxicity in the dog. Toxicologic Pathol, 26, 29-32, 1998.
6. Price RG: Early markers of nephrotoxicity. Comp Clin Path , 11, 2-7, 2002.
7. DAmico G, Bazzi C: Pathophysiology of proteinuria. Kidney Int, 63, 809-825, 2003.
8. Smets P M , Meyer E, Maddens BE, Duchateau L, Daminet S: Urinary markers in healthy young and aged dogs and dogs with chronic kidney disease. J Vet Intern Med, 24, 65-72, 2010.
9. Maddens B, Daminet S, Demeyere K, Demon D, Smets P, Meyer M: Validation of immunoassays for the candidate renal markers C-reactive protein, immunoglobulin G, thromboxane B 2 and retinol binding protein in canine urine. Vet Immunol Immunopathol, 134, 259-264, 2010.
10. Grauer GF, Oberhauser EB, Basaraba RJ, Lappin MR, Simpson DF, Jensen WA: Development of microalbuminuria in dogs with heartworm disease. J Vet Intern Med, 16, 352, 2002.
11. Lazarov L, Georgieva TM, Simeonova G, Zapryanova D, Nikolov J, Simeonov R: Markers of infammation in experimentally induced pancreatitis in dogs (Part I): Creactive protein and white blood cell counts. Revue Med Vet, 162, 118-122, 2011.
12. Zapryanova D, Mircheva T, Denev S: Plasma protein profiles and fibrinogen concentrations in dogs with experimentally induced Staphylococcus aureus infection. Revue Med Vet, 164, 150-155, 2013.
13. Maddens B, Daminet S, Smets P, Meyer E: Escherichia coli pyometra induces transient glomerular and tubular dysfunction in dogs. J Vet Intern Med, 24, 1263-1270, 2010.
14 . Polzin DJ: Chronic kidney disease. In, Ettinger SJ, Feldman EC (Eds): Textbook of Veterinary Internal Medicine. 1990-2021, St Louis, MO Saunders Elsevier, 2010.
15. Morchόn R, Carretόn E, Grandi G, González-Miguel J, Montova- Alonso JA, Simόn F, Genchi C, Kramer LH: Anti-Wolbachia surface protein antibodies are present in the urine of dogs naturally infected with Dirofilaria immitis with circulating microfilariae but not in dogs with occult infections. Vector Borne Zoonotic Dis, 12, 17-20, 2012.
16. Kramer L, Simόn F, Tamarozzi F, Genchi M, Bazzocchi C: Is Wolbachia complicating the pathological efects of Dirofilaria immitis infections. Vet Parasitol, 133, 133-136, 2005.
17. Paes-de-Almeida EC, Ferreira AM, Labarthe NV, Caldas ML, McCall JW: Kidney ultrastructural lesions in dogs experimentally infected with Dirofilaria immitis (Leidy, 1856). Vet Parasitol, 113, 157-168, 2003.