Tülay MUŞ ELAL, Füsun SONAT AK

The effect of orally administrated β-glucan and dietary restriction on faecal microfora in rats

Bu çalışmanın amacı oral yolla verilen β-glukan ve/veya diyet kısıtlamasının rat feçesindeki Lactobacillus spp., koliform, Enterobacteriaceae ve enterokok sayıları üzerine etkisini ortaya koymaktır. Bu amaçla ratlar üç deneysel gruba ayrıldı: i) birinci grup 6 ay süresince normal diyetle beslenen ve son 14 gün oral yolla β-glukan (20 mg/kg, canlı ağırlığa) verilen hayvanlar ii) ikinci grup 6 ay boyunca diyet kısıtlaması uygulanan ve ilk grupla aynı şekilde β-glukan verilen hayvanlar iii) yalnızca ad libitum beslenen kontrol grubu ratlardan oluşmaktadır. Kontrol grubu ile karşılaştırıldığında, ilk grupta koliform, Enterobacteriaceae ve laktobasil sayılarında artış gözlendi, fakat bu artış istatistiksel olarak önemli değildi. Koliform ve Enterobacteriaceae sayılarındaki artış yaklaşık 2 log iken, laktobasil sayılarında bu artış 1 log idi. İlginç olarak diyet kısıtlaması ile birlikte β-glukan uygulaması adı geçen bakterilerin artışında önemli bir etki oluşturmadı. Mevcut çalışmanın sonuçları, prebiyotik olarak geniş kullanım alanı bulunan β-glukanın oral yolla takviyesinin rat modelinde dışkıdaki mikroorganizmaları modifiye etme potansiyeline sahip olduğunu gösterdi.

Oral yolla uygulanan β-glukan ve diyet kısıtlamasının ratların fekal mikroforasına etkisi

In this study we aimed to evaluate the efect of orally administered β-glucan and/or dietary restriction on Lactobacillus spp., coliforms, Enterobacteriaceae and enterococci counts in rat faeces. For this aim, rats were divided into three experimental groups: i) first group animals received normal diet for 6 months and administrated orally with β-glucan (20 mg/kg for bodyweigth) over the last 14 day of experiment, ii) second group was dietary restricted animals for 6 months and receiving β-glucan as those of first group animals, iii) last group was the control group rats receiving only ad libitum feed. Compared to control group, numeric increase was observed in the number of coliforms, Enterobacriaceae and lactobacillus counts in first group but this was not statistically important. The increase in coliforms and Enterobacteriaceae counts was nearly 2 log while this was 1 log for lactobacillus counts. Interestingly, dietary restriction + β-glucan administration had no significant infuence on the increase of defined bacterial groups. The results of the present study showed that orally administration of the β-glucan, widely used as prebiotic, has the potential to modify faeces microbiota in rat model.

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