NAZLI ERCAN, Nevin TUZCU, ONUR BAŞBUĞ, Kurtuluş GÖK, HAKAN IŞIDAN, YUSUF ZİYA OĞRAK

The evaluation of important biomarkers in healthy cattle

Bu çalışmada veteriner hekimlikte enfeksiyonların teşhisinde ve prognozunun değerlendirilmesinde önemli faydalar sağlayacağı düşünülen prokalsitonin, neopterin, TNF α, IFN-γ, MDA, PGE2, IL-8 gibi biyolojik markerlerin farklı yaş gruplarında ve cinsiyetlerdeki sağlıklı sığırlardaki kan serumu seviyelerinin belirlenmesi amaçlanmıştır. Çalışmanın materyalini Sivas yöresinde bulunan işletmelerde yetiştirilen biyokimyasal ve hematolojik bulgularıyla sağlıklı olduğu belirlenen neonatal (24 ay) 48 adet (25 dişi 23 erkek) sığır ve buzağı oluşturmuştur. Serum prokalsitonin seviyesi, neonatal grupta genç ve ergin gruba göre düşük bulundu (P

Sağlıklı sığırlarda önemli biyomarkerların değerlendirilmesi

In this study the aim is to determine the blood serum levels of biological markers as procalcitonin, neopterin, TNF-α, MDA, PGE2, IL- 8, IFN-γ which are considered as highly beneficial on diagnosing the infections in the veterinary medicine and evaluating the prognosis in the healthy cattle at diferent ages and in diferent gender. The materials of this study are 48 (25 female and 23 male) cattle and calf bred (neonatal <1 month, young 12-24 month and mature >24 month) in operations in Sivas region and which are determined to be healthy via the biochemical and hematological findings. Serum procalcitonin level was found lower in neonatal group than the young and adult group (P<0.05). It was realized that neopterin level is reasonably higher in neonatal group than both young and adult group (P<0.05). MDA level in the young and adult group was measured as higher than of the neonatal group (P<0.05). There was found between the adult group and neonatal group a statistically reasonable diference for the serum PGE2 levels (P<0.05). In IL-8 level, there was found a statistically important diference only between young group and neonatal group (P<0.05). There was found no statistic diference among the levels of procalcitonin, neopterin, TNF-α, IFN-γ, MDA, PGE2, IL-8 between the sexes. As a result, it is concluded that determining the levels of markers used in defining the prognosis of the infection in healthy cattle at diferent ages would be a base data for further studies.

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1. Çöl R, Durgun Z: Sepsis, lökositler, sitokinler ve dissemine intravasküler koagukasyon. Vet Bil Derg , 23 (1): 97-106, 2007.
2. Baylan O, Albay A, Kısa Ö, Doğancı L: Prokalsitonin. Gülhane Askeri Tıp Akademisi Ayın Kitabı, sayı: 31, 2002.
3. Hisaeda K, Hagiwara K, Eguchi J, Yamanaka H, Kirisawa R, Iwai H: Interferon-gamma and tumor necrosis factor-α levels in sera and whey of cattle with naturally occuring coliform mastitis. J Vet Med Sci, 63 (9): 1009-1011, 2001.
4. Holter W, Goldman Ck, Casabo L, Nelson DL, Greene WC, Waldmann TA: Expression of functional IL 2 receptors by lipopolysaccharide and interferon-gamma stimulated human monocytes. J Immunol, 138, 2917- 2922, 1987.
5. Işık G, Demirezen Ş, Beksaç MS: Tümör nekroz faktör ve servikal kanser bağlantısı. Türk Bilimsel Derlemeler Dergisi, 1 (2): 55-61, 2008.
6. Erer H: Genel Patoloji Ders Notları. Teksir. Selçuk Üniversitesi Veteriner Fakültesi, Konya, 1986.
7. Başoğlu A, Şen İ, Sevinç M, Şimşek A: Serum concentrations of tumor necrosis factor-α in neonatal calves with presumed septicemia. J Vet Med, 18, 238-241, 2004.
8. Tünger Ö: Sepsisin tanı ve izlenimde prokalsitonin, CRP ve diğer göstergeler. Klimik Dergisi, 20, 1, 2007.
9. Baker DG, Gershwin J, Giri SN, Li C: Cellular and chemical mediators of type 1 hypersensivity in calves infected with ostertagia ostertagi: histamine, prostaglandin D2, prostaglandin E2 and leukotriene C4. Int J Parasitol, 23 (3): 333-339, 1993.
10. Tüközkan N, Erdamar H, Seven I: Measurement of total malondialdehyde in plasma and tissues by high-performanced liquid chromatography and thiobarbituric acid assay. Fırat Tıp Dergisi, 11 (2): 88-92, 2006.
11. Mukaida N, Okamoto S, Ishikawa Y, Matsushima K: Molecular mechanism of interleukin-8 gene expression. J Leukocyte Biol, 56, 554- 558,1994.
12. M artin H, Olander B, Norman M: Reactive hyperemia and interleukin 6, interleukin 8 and tumor necrosis factor-alpha in the diagnosis of early onset neonatal sepsis. Peadiatrics, 108, E61, 2001.
13. M ehr SS, Doyle LW, Rice GE, Vervaat P, Henschke P: Interleukin-6 and interleukin-8 in newborn bacterial infection. Am J Perinatol, 18, 313- 324, 2001.
14. S tang BV, Koller LD: Neopterin values in selected groups of normal animals. Res Vet Sci, 65, 87-88, 1998.
15. Assicot M, Gendrel D, Carsin H, Raymond J, Guilbaud J, Bohuon C: High serum procalcitonin concentrations in patients with sepsis and infection. Lancet, 341 (8844): 515-518, 1993.
16. Becker KL, Snider R, Nylen ES: Procalcitonin in sepsis and systemic inflammation: A harmful biomarker and a therapeutic target. Br J Pharmacol, 159, 253-264, 2010.
17. Önal Z, Önal H, Yıldız E, Yıldız CK, Şiraneci R: Prokalsitonin. Sendrom, 14 (12): 81-90, 2002.
18. Dandona P, Nix D, Wilson MF, Aljada A, Love J, Assicot M, Bohuon C: Procalcitonin increase after endotoxin injection in normal subjects. J Clin Endocrinol Metab, 79, 1605-1608, 1994.
19. Eksen M: Hematoloji. Selçuk Üniversitesi Basım Ünitesi, Konya, 1991.
20. Özdamar K: SPSS İle Biyoistatistik. Kaan Kitabevi. Eskişehir. ISBN: 975- 6787-03-1, 2001.
21. Demirdağ K, Özden M, Gödekmerdan A, Cihangiroğlu M, Kalkan A: Sepsis olgularında prokalsitonin, TNF-α ve C-reaktif protein düzeylerinin değerlendirilmesi. Klimik Dergisi, 16, 21-24, 2003.
22. Ertuğrul Ö, Ertuğrul MB: Prokalsitonin ve infeksiyon. Klimik Dergisi, 18 (2): 59-62, 2005.
23. Duncan JR, Prasse KW, Mahafey EA: Quality control, test validity and reference values. In, Veterinary Laboratory Medicine: Clinical Pathology. 3 rd ed, 235-238, Ames Iowa State University Press, 1994.
24. Ro dostits OM, Gay CC, Hinhclif KW, Constable PD: Appendix 2 Reference Laboratory values. In, Veterinary Medicine A Textbook of the Diseases of Cattle, Sheep, Goats, Pigs and Horses. 10 th ed., 2047-2050, WB Saunders, London,2006.
25. Günal Ö, Ulutan F, Erkorkmaz Ü: Sepsisli hastalarda prokalsitoninin prognostik değeri. Klimik Dergisi, 24 (1): 31-35, 2011
26. Castelli GPMD, Pognani CMD, Cita MMD, P RMD: Procalcitonin as a prognostic and diagnostic tool for septic complications after major trauma. Crit Care Med , 37 (6): 1845-1849, 2009.
27. P oyrazoğlu MH, Per H, Öztürk M, Bingöl N, Üzüm K: Çocukluk çağı pnömonilerinde serum prokalsitonin düzeyleri. Çocuk Sağlığı ve Hastalıkları Dergisi, 46, 169-176, 2002.
28. Fraccaro E, Coetzee JF, Odore R, Edwards-Callaway LN, Kukanich B, Badino P, Bertolotti L, Glynn H, Dockweiler J, Allen K, Bergamasco L: A study to compare circulating funixin, meloxicam and gabapentin concentrations with prostaglandin E2 levels in calves undergoing dehorning. Res Vet Sci, 95, 204-211, 2013.
29. Ergönül S, Aşkar T: Anaplasmosisli sığırlarda ısı şok protein (HSP), Malondialdehit (MDA), nitrik oksit (NO) ve interlökin (IL-6, IL-10) düzeylerinin araştırılması. Kafkas Univ Vet Fak Derg, 15 (4): 575-579, 2009.
30. Nisbet C, Yarım GF, Çiftci A, Çenesiz S, Çiftci G: Investigation of serum nitric oxide and malondialdehyde levels in cattle infected with Brucella abortus. Ankara Üniv Vet Fak, 54, 159-163, 2007.
31. Caswell JL, Middleton DM, Sorden SD, Gordon JR: expression pf the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurelosis. Vet Pathol, 35, 124-131, 1998.