To determine the occurence of afatoxin M1 (AFM1) in samples of cyprus traditional cheese (Halloumi): A Cross-Sectional study
Bu kesitsel çalışma ile Kıbrıs geleneksel peynir (Hellim) örneklerinde afatoksin M1 (AFM1) oluşumunu belirlemek için yapılmıştır. Çalışmada küme örnekleme yöntemiyle 36 endüstriyel yapımı ve 92 ev yapımı olmak üzere toplam 128 Hellim peyniri örneğe seçildi. Peynir örneklerindeki AFM1 varlığı ELISA yöntemi ile analiz edilerek belirlendi. Endüstri yapımı Hellim peynirinde AFM1 pozitif bulunma oranı %28.8 (0.00-16.66 ng/kg), ev yapımı Hellim peynirinde ise bu oran %21.7 (0.00-4.63 ng/kg) şeklinde düşük bir oran olduğu belirlendi. Endüstri ve ev yapımı Hellim peynirindeki AFM1 değerinin ortalama, standart hata (SEM) ve %95 güven aralığında sırasıyla 0.84±0.24, (%95 CI: 0.35-1.35) ve 1.28±0.32, (%95 CI: 0.63-1.93) şeklinde bulundu. Her iki ortalama arasındaki farklılık önemli bulunmadı (P=0.422) ve European Commission (EC) (250 ng/kg) and Turkish Food Codex (TFC) (500 ng/kg) değerlerinden önemli derecede düşük olduğu bulundu (p
Kıbrıs geleneksel peynir (Hellim) örneklerinde aflatoksin M1 (AFM1) oluşumunun belirlenmesi: Kesitsel bir çalışma
This Cross-Sectional present study was conducted to determine the occurence of afatoxin M1 (AFM1) in samples of Turkish Republic of North Cyprus (TRNC) traditional cheese (halloumi). In the current study a total of 128 halloumi cheese samples including 36 industrial made and 92 home made were selected by using cluster sampling method and analyzed for AFM1 with the competitive ELISA. The percent of AFM1 contamination in halloumi cheese was found to be low, since 28.8% (0-16.66 ng/kg) of the samples were positive in industrial made and 21.7% (0-4.63 ng/kg) in home made. The mean, standard error of mean (SEM), 95% Confidence Interval (95% CI) values of afatoxin M1 in halloumi cheese with industrial and home made samples were 0.84±0.24, (95% CI :0.35-1.35) and 1.28±0.32, (95% CI: 0.63-1.93) respectively. Both means were not significantly diference (P=0.422) and found very low from the limits of European Commission (EC) (250 ng/kg) and Turkish Food Codex (TFC) (500 ng/kg) (P<0.001). In order to prevent from introduction of afatoxin M1 into cheese industry cycle, hygienic conditions, appropriate storage and control of livestock feed at all stages of planting and requires system that makes afatoxin control are necessary.
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- 1. Hellim Tanıtım Grubu: Hellim/Halloumi. http://hellimhalloumi.org, Accessed: 10 March 2014.
- 2. Darsanaki RK, Mohammadi M, Kolavani MH, Issazadeh K, Aliabadi MA: Determination of afatoxin M 1 levels in raw milk samples in Gilan, Iran. Adv Stud Biol, 5 (4): 151-156, 2013.
- 3. Motawee MM: Reduction of afatoxin M1 content during manufacture and storage of Egyptian Domaiti Cheese. Int J Vet Med Res Rep, 2013, 1-11, 2013.
- 4. Ayoub MM, Mahmoud KSA, Amal AR: Evaluation of afatoxin M 1 in raw, processed milk and some milk products in Cairo with special reference to its recovery. Researcher, 3 (9): 5-11, 2011.
- 5. Mohamadi SMA, Khezri M, Moradnia H: Determination of afatoxin M1 in milk by ELISA technique in Mashad (Northeast of Iran). Int Schol Res Network ISRN Toxicolog, 2012, 1-4, 2012.
- 6. Liu Y, Wu F: Global burden of aflatoxin-ınduced hepatocellular carcinoma: A risk assessment. Environ Health Persp, 118, 818-824, 2010.
- 7. World Health Organization (WHO): The Global Burden of Disease: 2004 Update. Geneva: 2008. http://www.who.int/healthinfo/global_burden_ disease/2004_report_update/en/index.html. Accessed: 10 March 2014.
- 8. Wild CP, Gong YY: Mycotoxins and human disease: A largely ignored global health issue. Carcinogenesis, 31 (1): 71-82, 2010.
- 9. Manetta AC, Giammarco M, Di Giuseppe L, Fusaro I, Gramenzi A, Formigoni A, Vignola G, Lambertini L: Distribution of afatoxin M 1 during Grana Padano cheese production from naturally contaminated milk. Food Chemistry, 113 (2): 595-599, 2009.
- 10. Rubio R, Moya VJ, Berruga MI, Molina MP: Afatoxin M 1 in the intermediate dairy products from Manchego cheese production: Distribution and stability. Mljekarstvo, 61 (4): 283-290, 2011.
- 11. Anfossi L, Baggiani C, Giovannoli C, DArco G, Passini C, Giraudi G: Occurrence of aflatoxin M 1 in Italian cheese: Results of a survey conducted in 2010 and correlation with manufacturing, production season, milking animals, and maturation of cheese. Food Control, 25 (1): 125-130, 2012.
- 12. Grace D: Animals and afatoxins. In, Unnevehr L, Grace D (Eds): Afatoxins Finding Solutions for Improved Food Safety. 16-18, Int. Food Policy Res Inst, USA, 2013. http://www.ifpri.org/sites/default/files/ publications/focus20.pdf, Accessed: 10 March 2014.
- 13.Taniwaki MH, Hocking AD, Pitt JI, Fleet GH: Growth of fungi and mycotoxin production on cheese under modified atmospheres. Int J Food Microbiol, 68 (1-2): 125-133, 2001.
- 14. Hocking AD, Faedo M: Fungi causing thread mould spoilage of vacuum packaged cheddar cheese during maturation. Int J Food Microbiol, 16 (2): 123-130, 1992.
- 15. European Commission (EC): No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstufs. Of J Eur Union , 364, 5-24, 2006.
- 16.Türk Gıda Kodeksi (TGK): Gıda maddelerinde belirli bulaşanların maksimum seviyelerinin belirlenmesi hakkında tebliğ. Resmi Gazete, Sayı: 26879, 17 Mayıs 2008.
- 17. Motawee MM, McMahon DJ: Fate of afatoxin M1 during manufacture and storage of feta cheese. J Food Sci, 74 (5): 42-47, 2009.
- 18. Prado G, Oliveira MS, Lima AS, Moreira APA: Occurrence of afatoxin M1 in parmesan cheese consumed in Minas Gerais, Brazil Ciênc, 32 (6): 1413-1418, 2008.
- 19.Rubio R, Moya VJ, Berruga MI, Molina MP, Molina A: Afatoxin M 1 in the intermediate dairy products from Manchego cheese production: Distribution and stability. Mljekarstvo , 61 (4): 283-290, 2011.
- 20. Turgay O, Aksakal DH, Sunnetci S, Celik AB: A survey of afatoxin M1 levels in Kahramanmaraş cheese. Turk J Vet Anim Sci, 34 (6): 497-500, 2010.
- 21. Oruc HH, Cibik R, Yilmaz E, Kalkanli O: Distribution and stability of afatoxin M1 during processing and ripening of traditional white pickled cheese. Food Addit Contam , 23 (2): 190-195, 2006.
- 22. Atasever MA, Adiguzel G, Atasever M, Ozturan K: Determination of afatoxin M1 levels in some cheese types consumed in Erzurum - Turkey. Kafkas Univ Vet Fak Derg , 16 (Suppl-A): S87-S91, 2010.
- 23.Rahimi E, Anari MM, Alimoradi M, Rezaei P: Aflatoxin M1 in pasteurized milk and white cheese in Ahvaz, Iran. Global Veterinaria , 9 (4): 384-387, 2012.
- 24.Filazi A, Ince S, Temamogullari F: Survey of the occurrence of afatoxin M 1 in cheeses produced by dairy ewes milk in Urfa city, Turkey . Ankara Üniv Vet Fak Derg, 57, 197-199, 2010.
- 25. Lopez C, Ramos L, Ramadan S, Bulacio L, Perez J: Distribution of afatoxin M1 in cheese obtained from milk artificially contaminated. Int J Food Microbiol, 64, 211-215, 2001.
- 26. Aksoy A, Yavuz O, Guvenc D, Das YK, Terzi G, Celik S: Determination of afatoxin levels in raw milk, cheese and dehulled hazelnut samples consumed in Samsun province, Turkey. Kafkas Univ Vet Fak Derg , 16 (Suppl-A): S 13- S 16, 2010.
- 27.Atasever MA, Adiguzel G, Atasever M, Ozlu H, Ozturan K: Occurrence of afatoxin M1 in UHT milk in Erzurum-Turkey. Kafkas Univ Vet Fak Derg , 16 (Suppl A): S119-S122, 2010.
- 28. Karakaya Y, Atasever M: Afatoxin B 1 in corn silage and its probability passing in milk. Kafkas Univ Vet Fak Derg, 16 (Suppl-A): S123-S127, 2010.
- 29.Tekinsen KK, Tekinsen OC: Afatoxin M 1 in white pickle and Van otlu (herb) cheeses consumed in southeastern Turkey. Food Control, 16 (7): 565-568, 2005.
- 30.Kıvanç M: Mold growth and presence of afatoxin in some Turkish cheeses. J Food Safety, 10 (4): 287-294, 1990.
- 31.Oliveira CAF, Franco RC, Rosim RE, Fernandes AM: Survey of aflatoxin M1 in Minas cheese from the North-East region of São Paulo, Brazil. Food Addit Contam: Part B: Surveillance, 4 (1): 57-60, 2011.
- 32. López C, Ramos L, Ramadan S, Bulacio L, Perez J: Distribution of aflatoxin M1 in cheese obtained from milk artificially contaminated. Int J Food Microbiol, 64 (1-2): 211-215, 2001.
- 33. Talebi E, Khademi M, Rastad A: An over review on efect of afatoxin in animal Husbandry. Asian J Exp Biol Sci, 2 (3): 754-757, 2011.