SAVAŞ YILDIZ, YAVUZ ÖZTÜRKLER, UMUT ÇAĞIN ARI, NECDET CANKAT LEHİMCİOĞLU, EMİNE ATAKİŞİ, RECAİ KULAKSIZ

The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen

Bu çalışmanın amacı, koçlarda L-Ergothionin (LE), sistein ve N-asetil sisteinin (NAC) farklı dozlarının çözüm sonu sperma parametreleri üzerine etkisini belirlemekti. Dört Tuj ırkı koçtan alınan ejakülatlar değerlendirildi ve 35°Cde karışım yapıldı. Sperma örnekleri, LE (5, 10 mM), Sistein (5, 10 mM), NAC (5, 10 mM) içeren veya antioksidan içermeyen (kontrol), yağsız süt temelli sulandırıcıyla sulandırılarak, 0.25 mllik payetlere dolduruldu. Payetler 5°Cye 2 saat süreyle soğutuldu, sıvı azot buharında (yaklaşık -120°C) 15 dak. süre ile donduruldu ve çözme işlemine kadar sıvı azot içinde saklandı. Payetler su banyosunda (37°Cde 1 dak.) çözüldü. Motilite, canlılık, anormal akrozom, toplam anormalite ve membran bütünlüğü (hipoozmotik şişme testi, HOST) yüzdeleri istatistiksel olarak değerlendirildi. Ayrıca, toplam antioksidan kapasite (TAK) ve toplam oksidatif stres (TOS), replikasyonlardan elde edilen örneklerde değerlendirildi. LEnin motilite (sistein hariç), canlılık, akrozom hasarı, total morfolojik anormalite, HOST ve TAK oranları bakımından, NAC ve sisteine üstünlük sağladığı görüldü (P0.05). Sistein ve NACın koçlarda çözüm sonu spermatolojik parametreler üzerine bazı olumsuz etkileri belirlenirken, LEnin olumlu ya da olumsuz etkisinin olmadığı sonucuna varıldı. Bu sonuçlar, koç spermasının dondurulmasında, LE, NAC ve sistein kullanılması üzerine yeni bilimsel çalışmaların yapılması gerektiğini göstermektedir.

Koç spermasının dondurulması üzerine L-Ergothionin, N-asetil sistein ve sisteinin etkileri

The aim of this study was to determine the infuence of diferent doses of L-Ergothioneine (LE), cystein and N-acetylcystein (NAC) on post-thawing semen parameters in rams. Ejaculates collected from four Tushin Rams were evaluated and pooled at 35°C. Semen samples were diluted with skim-milk based extender containing LE (5, 10 mM), Cystein (5, 10 mM), NAC (5, 10 mM) or not containing any antioxidant (control) and loaded to 0.25 ml French straws. Straws were cooled to 5°C for 2 h, frozen in liquid nitrogen vapour (aprox. -120°C) for 15 min and then being stored in liquid nitrogen until thawing process. Straws were thawed in water bath (37°C for 1 min). The percentages of motility, viability, abnormal acrosome, total abnormalities, membrane integrity (hypoosmatic swelling test, HOST) were statistically assessed. Also total antioxidant capacity (TAC) and total oxidatif stres (TOS) were evaluated in samples from replications. It was seen that LE was superior to N-acetylcystein and cystein in motility, viability, defected acrosome, total morphological abnormalities, HOST and TAC, (P<0.05) except cystein in motility. Neverthless, there was not any statistically diference between LE and control groups (P>0.05). It was conlcluded that there was not any benefcial or detrimental efects of LE on post thawing semen parameters in rams while it was determined that cystein and NAC may have been some detrimental efects on post thawing semen parameters in rams. These results warrants future scientifc studies on LE, NAC and cystein in ram semen cryopreservation.

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