ELÇİN GÜNAYDIN, HAMİT KAAN MÜŞTAK, BARIŞ SAREYYÜPOĞLU, Zafer ATA

PCR detection of Coxiella burnetii in fetal abomasal contents of ruminants

Bu çalışma, İç Anadolu ve Karadeniz Bölgesinden laboratuvara gönderilen 102 sığır, 45 koyun ve 5 keçi atık fötus mide içeriğinde trans-PCR ile Coxiella burnetii (C. burnetii) varlığını araştırmak için yapıldı. Yüz elli iki abomasal içeriğin, 11inin (%7.23) C. burnetii DNAsı içerdiği tespit edildi. 102 sığırın 4 (%3.92)ünün, 45 koyunun 5 (%11.11)inin ve 5 keçinin 2 (%40)sinin abomasal içeriklerinde C. burnetii DNAsı tespit edildi. Sonuç olarak, fetal abomasal içeriklerde PCR ile C. burnetii DNAsı tespit edilmesinin, C. burnetii ilişkili abortlar için önemli bir bulgu olduğu düşünüldü.

Ruminantların fötal abomasal içeriklerinde Coxiella burnetiinin PCR ile tespiti

This study was carried out on abomasal contents of aborted fetuses of 102 cattles, 45 sheep and 5 goats sent to the laboratory from Central Anatolia, and Black Sea Region of Turkey to investigate the presence Coxiella burnetii (C. burnetii) by trans-PCR. Out of 152 abomasal contents, 11 (7.23%) were detected to contain C. burnetii DNA. Four (3.92%) out of 102 cattle, 5 (11.11%) out of 45 sheep, and 2 (40%) out of 5 goat abomasal contents harbored C. burnetii DNA. As a result, PCR detection of C. burnetii DNA in fetal abomasum contents is a significant finding of a possible C. burnetii related abortion.

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1. Porter RS, Czaplicki G, Mainil J, Guatteo R, Saegerman C: Q Fever: Current state of knowledge and perspectives of research of a neglected zoonosis. Int J Microbiol, 248418, 1-22, 2011.
2. Brouqui P, Marrie T, Raoult D: Coxiella. In, Murray P, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA (Eds): Manual of Clinical Microbiology. 9 th ed., 1062-1069, Washington, DC: ASM Press; 2007.
3. Cutler SJ, Bouzid M, Cutler RR: Q fever. J Infect, 54, 313-318, 2007.
4. Field PR, Mitchell JL, Santiago A, Dickeson DJ, Chan SW, Ho DWT, Murphy AM, Cuzzubbo AJ, Devine PL: Comparison of a commercial enzyme-linked immunosorbent assay with immunofluorescence and complement fixation tests for detection of Coxiella burnetii (Q fever) immunoglobulin M. J Clin Microbiol, 38, 1645-1647, 2000.
5. Anderson A, Bijlmer H, Fournier PE, Graves S, Hartzell J, Kersh GJ, Limonard G, Marrie T, Massung RF, McQuiston JH, Nicholson WL, Paddock CD, Sexton DJ: Diagnosis and management of Q Fever-United States, 2013: Recommendations from CDC and the Q Fever working group. MMWR , 62, 35, 2013.
6. World Organization for Animal Health (OIE): Q fever. In, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 7 th ed., Paris, Office International Des Epizooties. 2010.
7. Berri M, Laroucau K, Rodolakis A: The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by the use of a single touchdown polymerase chain reaction. Vet Microbiol, 72, 285-293, 2000.
8. Stein A, Raoult D: Detection of Coxiella burnetti by DNA amplification using PCR. J Clin Microbiol, 30, 2462-2466, 1992.
9. To H: Prevalence of Coxiella burnetii infection in dairy cattle with reproductive disorders. J Vet Med Sci, 60, 859-861, 1998.
10. Zhang GQ, Hotta A, Mizutani M, Ho T, Yamaguchi T, Fukushi H, Hirai K: Direct identification of Coxiella burnetii plasmids in human sera by nested PCR. J Clin Microbiol, 36, 2210-2213, 1998.
11. Brennan RE, Samuel JE: Evaluation of Coxiella burnetii antibiotic susceptibilities by real-time PCR assay. J Clin Microbiol, 41, 1869-1874, 2003.
12. Klee SR, Tyczka J, Ellerbrok H, Franz T, Linke S, Baljer G, Appel B: Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii. BMC Microbiol, 6, 2, 2006.
13. Panning M, Kilwinski J, Greiner-Fischer S, Peters M, Kramme S, Frangoulidis D, Meyer H, Henning K, Drosten C: High throughput detection of Coxiella burnetii by real-time PCR with internal control system and automated DNA preparation. BMC Microbiol, 8, 77, 2008.
14. Nguyen SV, Hirai K: Diferentiation of Coxiella burnetii isolates by sequence determination and PCR-restriction fragment length poly- morphism analysis of isocitrate dehydrogenase gene. FEMS Microbiol Lett, 180, 249-254, 1999.
15. Heinzen RA, Frazier ME, Mallavia LP: Nucleotide sequence of Coxiella burnetii superoxide dismutase. Nucleic Acids Res, 18, 6437, 1990.
16. Hoover TA, Vodkin MH, Williams JC: A Coxiella burnetii repeated DNA element resembling a bacterial insertion sequence. J Bacteriol, 174, 5540-5548, 1992.
17. Cetinkaya B, Kalender H, Ertas HB, Muz A, Arslan N, Ongor H, Gurçay M: Seroprevalence of coxiellosis in cattle, sheep and people in the east of Turkey. Vet Rec, 146, 131-136, 2000.
18. Gazyagci S, Aktas MS, Kilic S, Babur C, Celebi B, Duru SY: Sero- prevalence of Q fever in dairy cattle in the Konya province, Turkey. Revue Méd Vét, 162, 387-390, 2011.
19. Gozalan A, Esen B, Rolain JM, Akin L, Raoult D: Is Q fever an emerging infection in Turkey? East Mediterr Health J, 11, 384-391, 2005.
20. Kennerman E, Rousset E, Gölcü E, Dufour P: Seroprevalence of Q fever (coxiellosis) in sheep from the Southern Marmara Region, Turkey. Comp Immunol Microbiol Infect Dis, 33, 37-45, 2010.
21. Ozturk D, Kale M, Pehlivanoglu F, Hasiroglu S, Turutoglu H: Evaluation for some bacterial and viral abortions of dairy cattle farms in Burdur District of Turkey. Kafkas Univ Vet Fak Derg , 18 (2): 255-258, 2012.
22. Kirkan S, Kaya O, Tekbiyik S, Parin U: Detection of Coxiella burnetii in cattle by PCR . Turk J Vet Anim Sci, 32, 215-220, 2008.
23. Ongor H, Çetinkaya B, Karahan M, Açık MN, Bulut H, Muz A: Detection of Coxiella burnetii by immunomagnetic separation-PCR in the milk of sheep in Turkey. Vet Rec , 154, 570-572, 2004.
24. Dogru Kasimoglu A, Yildirim M, Unal N, Gazyagci S: The Relationship of Coxiella burnetii seropositivity between farm animals and their owners: A pilot study. JAVA, 9, 1625-1629, 2010.
25. Can tas H, Muwonge A, Sareyyupoglu B, Yardimci H, Skjerve E: Q fever abortions in ruminants and associated on-farm risk factors in northern Cyprus. BMC Vet Res, 7, 13, 2011.
26. Van den Brom R, van Engelen E, Vos J, Luttikholt SJM, Moll L, Roeast HIJ, van der Heijden HMJF, Vellema P: Detection of Coxiella burnetii in the bulk tank milk from a farm with vaccinated goats, by using a specific PCR technique. Small Rumin Res, 110, 150-154, 2013.
27. Vaidya VM, Malik SVS , Kaur S , Kumar S, Barbuddhe SB: Comparison of PCR, immunofuorescence assay, and pathogen isolation for diagnosis of Q fever in humans with spontaneous abortions. J Clin Microbiol, 46, 2038-2044, 2008.
28. Dehkordi FS: Prevalence study of Coxiella burnetii in aborted ovine and caprine fetuses by evaluation of nested and real-time PCR assays. Am J Anim Vet Sci, 6, 180-186, 2011.
29. Guatteo R, Joly A, Beaudeau F: Shedding and serological patterns of dairy cows following abortions associated with Coxiella burnetii DNA detection. Vet Microbiol, 155, 430-433, 2012.
30. Kampschreur LM , Oosterheert JJ, Koop AM , Wegdam-Blans MC, Delsing CE , Bleeker-Rovers CP, De Jager-Leclercq MG, Groot CA, Sprong T, Nabuurs-Franssen MH , Renders NH, van Kasteren ME, Soethoudt Y, Blank SN, Pronk MJ, Groenwold RH , Hoepelman AI, Wever PC: Microbiological challenges in the diagnosis of chronic Q fever. Clin Vaccine Immunol, 19, 787-790, 2012.
31. Schneebereger PM, Hermans MHA, van Hannen EJ, Schellekens JJA, Leenders ACA, Wever PC: Real-Time PCR with serum samples is dispensable for early diagnosis of acute Q fever. Clin Vac Immunol, 2, 286-290, 2010.
32. Milazzo A, Hall R, Storm PA, Harris RJ, Winslow W, Marmion BP: Sexually transmitted Q fever. Clin Infect Dis, 33, 399-402, 2001.
33. Bouvery NA, Souriau A, Lechopier P, Rodolakis A: Experimental Coxiella burnetii infection in pregnant goats: excretion routes. Vet Res, 34, 423-433, 2003.
34. Masala G, Porcu R, Sanna G, Chessa G, Cillara G, Chisu V, Tola S: Occurrence, distribution, and role in abortion of Coxiella burnetii in sheep and goats in Sardinia, Italy. Vet Microbiol, 99, 301-305, 2004.
35. Rodolakis A, Berri M, Héchard C, Caudron C, Souriau A, Bodier CC, Blanchard B, Camuset P, Devillechaise P, Natorp JC, Vadet JP, Arricau- Bouvery N: Comparison of Coxiella burnetii shedding in milk of dairy bovine, caprine, and ovine herds. J Dairy Sci, 90: 5352-5360, 2007.
36. Roest HJ, van Gelderen B, Dinkla A, Frangoulidis D, van Zijderveld F, Rebel J, van Keulen L: Q fever in pregnant goats: Pathogenesis and excretion of Coxiella burnetii. PLoS One , 7 (11): e48949, 2012.
37. Roest HI, Ruuls RC, Tilburg JJ, Nabuurs-Franssen MH, Klaassen CH: Epidemiology of Coxiella burnetii from ruminantsin Q Fever outbreak Netherlands. Emerg Infect Dis, 17, 668-675, 2011.
38. Oporto B, Barandika JF, Hurtado A, Aduriz G, Moreno B: Incidence of ovine abortion by Coxiella burnetii in northern Spain. Ann N Y Acad Sci, 1078, 498-501, 2006.
39. Parisi A, Fraccalvieri R, Cafiero M, Miccolupo A, Padalino I, Montagna C, Capuano F, Sottili R: Diagnosis of Coxiella burnetii-related abortion in Italian domestic ruminants using single-tube nested PCR. Vet Microbiol, 26, 101-106, 2006.
40. Jones RM, Twomey DF, Hannon S, Errington J, Pritchard GC, Sawyer J: Detection of Coxiella burnetii in placenta and abortion samples from British ruminants using real-time PCR. Vet Rec , 18, 965-967, 2010.
41. Nakoune E, Debaere O, Koumanda-Kotogne F, Selekon B, Samory F, Talarmin A: Serological surveillance of brucellosis and Q fever in cattle in the Central African Republic. Acta Trop, 92, 147-151, 2004.
42. Sargison N: 2008. Sheep Flock Health: A Planned Approach. Blackwell Publishing Ltd, Oxford, UK, 1-457.