ABİT AKTAŞ, M.Başak ULKAY, H. Hakan BOZKURT

The effects of Genistein on the cockerel testis during embryonic development

Bu çalışmada, embriyonik gelişim sırasında uygulanan genisteinin civciv testisleri üzerinde göstereceği etkilerin belirlenmesi amaçlandı. İnkübasyonun 4. gününde, soya fasulyesinden elde edilmiş genistein, yumurtanın küt ucundan küçük bir delik açılarak yumurta sarısına enjekte edildi. Çıkımdan 3 gün sonra erkek ciccivlerin testisleri alınarak paraffin kesitleri hazırlandı. Standart nokta sayım yöntemiyle Sertoli hücreleri ve germ hücrelerinin nukleus hacim yoğunlukları ve toplam hacimleri belirlendi. Çalışma sonucunda embriyonik dönemde uygulanan genisteinin, civciv testisleri üzerinde olumsuz bir etki göstermediği saptandı. Aksine germ hücrelerinin gelişimini olumlu yönde etkilediği belirlendi.

Anahtar Kelimeler:

horoz, embriyonik gelişim, genistein, eşey hücreleri, testis

Genisteinin embriyonik gelişim sırasında civciv testisleri üzerine etkisi

There is no in vivo study on the effect of phytoestrogens on the development of testis in domestic fowl; therefore the aim of this study was to examine the effect of genistein on the cockerel testis during embryonic development. Genistein from soybean were injected into yolk via a small hole at the blunt end of the egg on day 4 of incubation. Cockerels were decapitated on post-hatching day 3 and paraffin sections of the testes were prepared. Nuclear volume density and absolute volume of Sertoli cells and germ cells, seminiferous tubule volume density and absolute volume were determined using the standard point counting method. Genistein did not cause any inhibitory effect on the embryonic development of cockerel testis. On the contrary it had a stimulatory effect on germ cell development.

Keywords:

cocks, embryonic development, genistein, germ cells, testes,

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