Feyzan Özdal KURT, SERDAR KOCA

Bazı Batı Anadolu Triturus türleri (Salamandridae, Urodela) üzerinde sitogenetik bir çalışma

Bu çalışmada Salamandridae familyasına ait iki Triturus türünün (T. vulgaris ve T. karelinii) kromozom yapı ve sayıları incelenip karyotipleri çıkarılmıştır. Sitogenetik incelemeler sonucunda her iki türün kromozom sayısı 2n=24 olarak saptanmıştır. Karyotipi oluşturan kromozomlar büyük, orta ve küçük olmak üzere üç grupta toplanmıştır. T. vulgaris’ te metasentrik morfolojideki I, VIII ve XII. kromozom çiftleri; T. karelinii’de submetasentrik, submetasentrik yapıda olan VI, VII, IX, X ve XI. kromozom çiftleri ise metasentriktir. Diğer II, III, IV ve V. Kromozom çiftleri her iki türde de metasentrik morfolojide bulunmuştur. Ölçüm sonuçlarımıza göre T. karelinii’nin kromozomları daha büyük ve daha düzenli sıra halinde olup kromozomlar arasındaki intrakaryotipik varyasyon daha düşüktür.

Anahtar Kelimeler:

kromozomlar, sitogenetik, karyotip, Türkiye

A Cytogenetic study on the some Western Anatolian Triturus species (Salamandridae, Urodela)

The karyotypes of two species in the name of Triturus vulgaris and Triturus karelinii, belonging Salamandridae family of Urodela order were analyzed. Chromosome numbers of both the Triturus species were identified as 2n=24 and chromosomes forming karyotypes clustred in three group, long, medium and short by their lengths. I, VIII and XIIth chromosome pairs in Triturus vulgaris showed metacentric morphology whereas the same chromosomes showed submetasentric morphology in Triturus karelinii. VI, VII, IX, X and XIth chromosome pairs in Triturus vulgaris were found to be submetasentric compared to metasentric morphology of same chromosome pairs in Triturus karelinii. The other chromosome pairs II, III, IV and Vth were found to have metasentric morphology in both species. According to our measurements, chromosomes of Triturus karelinii were found to be long and to have more regular line and intrakaryotypic variations between chromosomes were determined as low.

Keywords:

chromosomes, cytogenetics, karyotypes, Turkey,

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Sessions SK: Evolutionary cytogenetics in Salamanders. Chromosome Research, 16, 183-201, 2008.
Bishop SC: Handbook of Salamanders. Comstock Publishing Company, New York, 1947.
Terentjev PV, Chemov SA: Key to Amphibians and Reptiles. Israel Program for Scientific Translation, Ierusa1em (3rd ed.) Trans. L. Kochua,1965.
Thorn R: Les Sa1amandres d'Europe, d' Asie et d' Afuque du Nord,b Editions Paul Lechevalier, Paris, 1968.
Baran İ: Türkiye Amfibi ve Sürüngenleri. TÜBİTAK Popüler Bilim Kitapları 207, V+165 sayfa ISBN 975- 403.356.0, Pelin Matbaası/Ankara, 2005.
Levan A, Fredga K, Landberg A: Nomenc1ature for Sentromeric Position Chromosomes. Hereditas, 52, 201- 220, 1964.
Caspersson T, Zech L, Johanson C: Differantial banding of Alkylating Fluorochromes in Human Chromosomes. Exp Cell Res, 60, 315-319, 1970.
Sumner AT: A simple technique to demonstrating centromeric heterochromatin. Exp Cell Res, 75, 304-306, 1972.
Wolf S, Perry P: Differential Giemsa staining of sister chromatid and the study of sister chromatid exchange without autoradyography. Chromosoma (Berl), 48, 341- 353, 1974.
Goodpasture C, Bloom SE: Visualisation of nucleolar organiser region in mammalian chromosomes using silver staining. Chromosoma (Berl), 53, 37-50, 1975.
Schmid M: Chromosome banding in amphibia I. Constituve heterochromatin and nucleolus organizer regionsin Bufo and Hyla. Chromosoma, 66, 361-388,1978a.
Schmid M: Chromosome banding in amphibia II. Constituve heterochromatin nucleolus organizer regions in Ranidae, Microhylidae and Rhacophoridae. Chromosoma, 68, 131-148, 1978b.
Herrero P: Karyotypes of two Iberian amphibians: Rana iberica (Anura: Ranidae) and Triturus boscai (Caudata: Salamandridae). Herpetologica, 38 (4): 502-506, 1982.
Herrero P, Arano B: Cytogenetic and morphological studies on Triturus alpestris cyreni. In, Rocek Z (Ed): Studies in Herpetology. 151-154, 1986.
Ragghianti M, Bucci-Innocenti S, Mancino G: Karyology of Neurergus strauchii (Steindachner, 1887) (Urodela: Salamandridae) and cytotaxonomic considerations on Palearctic salamandrids. Amphibia-Reptilia, 8, 101-110,1987.
Herrero P, Arano B, Garcia de la Vega C: Chromosome differentation in the Triturus alpestris complex (Ampibia: Caudata). Genetica, 79, 27-35, 1989.
Bucci-Innocenti S, Ragghianti M, Mancino G: Investigation of karyology and hybrids in Triturus boscai and T. viIlatus, with a reinterpretation of the species groups within Triturus (Caudata:Salamandridae). Copeia, 3, 662-672, 1983.
Stephenson EM, Robinson ES, Stephenson NS: Karyotype variation within the genus Leiopelma (Amphibia; Anura). Can J Genet Cytol, 14, 691-702, 1972.
Green DM, Bogart JP, Anthony EH, Genner DL: An interactive, microcomputer-based karyotype analysis system for phylogenetic cytotaxonomy. Compui Biol Med, 10, 219-227, 1980.
Werner F: Die Reptilien-und Amphibienfauna von Kleinasien. Sitz Ber Akad Wiss Wien, Mathemat-Naturwiss KI, Abt I, 111, 1057-1121, 1902.
Wolterstorff W: Über Triton (=Molge) vulgaris subsp. Typica Von Konstatinopel. Bl Aquar Terr Kde, 19, 439-422, 1908.
Venzmer G: Neues Verzeichnis der Amphibien und Reptilien von Kleinasien. ZoolJahrb Syst, 46, 43-60,1922.
Bodenheimer FS: Introduction into the knowledge of the amphibia and reptilia of Turkey. İstanbul Üniv Fen Fak Mecm Ser, B, 9, 1-78, 1944.
Özeti N: Ege Bölgesinde bulunan Triturus vulgaris (Linnaeus)'un morfolojisi, taksonomik durumu, mevsimlik faaliyeti ve teromotaktik davranisi uzerinde arastirmalar. Ege Üniv Fen Fak İlmi Rap Ser No: 15 (in Turkish), 1964.
Namur P: Etude des Chromosomes de La Blastula de Triturus vulgaris Linne. Extrait du Bulletin de La Societe Linxeenne de Normandie, 10, 67-72, 1969.
Barsacchi G, Bussotti L, Mancino G: The maps of the lampbrush chromosomes of Triturus (Amphibia: Urodela) N. Triturus vulgaris meridionalis. Chromosoma (Berlin), 31, 255-279, 1970.
Callan HG: Heterochromatin in Triton. Proc Roy Soc Lond, B 130, 324-335, 1942.
Schmid M, Olert J, Klett C: Chromosome banding in amphibia. III. Sex chromosomes in Triturus. Chromosoma (Berlin), 71, 29-55, 1979.
Green DM, Sessions SK: Amphibian Cytogenetics and Evolution. San Diego: Academic Pres, pp 189-190, 1991.
Macgregor HC, Sessions SK, Arntzen JW: An integrative analysis of phylogenetic relationships among newts of the genus Triturus (Family Salamandridae), using comparative biochemistry, cytogenetics and reproductive interactions. J Evolutionary Biol, 3, 329-373, 1990.
Sims SH, Macgregor H, Pellatt PS, Homer HA: Chromosome 1 in crested and marbled newts (Triturus). An extraordinary cage of heteromorphism and independent chromosome evolution. Chromosoma (Berlin), 89, 169-185, 1984.