The Effects of Different Zinc Sources and Microbial Phytase Supplementation on the Tibial Bone Properties, Strength and Zn Mineralization Broilers Fed with Diet Low Phosphorus [1]

Bu araştırma, düşük yararlanılabilir fosfor (Py) içeren mısır-soya temeline dayanan diyetlere farklı çinko kaynakları ile mikrobiyal fitaz ilavesinin broylerlerde tibia morfometrik indeksler, kemik direnci ve çinko birikimi üzerine etkisini değerlendirmek amacıyla yapılmıştır. Araştırma kırk iki gün sürmüştür. Toplam 875 adet günlük Ross 308 civciv, yedi farklı deneme grupları ve her grup 25 adet erkek hayvan içeren beş alt gruptan oluşturulmuştur. Pozitif kontrol grubu, çinko içermeyen mineral ön karması ve yeterli miktarda yararlanılabilir fosfor (%0.45) içeren rasyonla beslenmiştir. Negatif kontrol grubu ise, çinko içermeyen mineral ön karması ve düşük fosfor (%0.30) içeren temel bir rasyonla beslenmiştir. Deneme grupları; Fitaz, organik çinko, inorganik çinko, organik çinko + fitaz ve inorganik çinko + fitaz rasyonlarına sırasıyla %0.30 Py ve 500 FTU fitaz/kg; %0.30 Py ve 75 mg/kg Zn-proteinat; %0.30 aP ve 75 mg/kg ZnSO4; 75 mg/kg Zn-proteinat ve 500 FTU fitaz/kg; 75 mg/kg ZnSO4 ve 500 FTU fitaz/kg katılmıştır. Tibiotarsal diafiz çapı, medial duvar kalınlığı, tibiotarsal indeksi, medullar kanal çapı, esnekliği ve kırılma direnci bakımından gruplar arasında bir farklılık bulunmamıştır. Bununla beraber, OZ + PH and IZ + PH rasyon grubuna ait tibiotarsal ağırlık, tibiotarsal ağırlık/uzunluk indeksi değerleri her iki kontrol grubundan önemli düzeyde farklılık (P

Değişik Çinko Kaynakları ve Mikrobial Fitaz Katkısının Düşük Düzeyde Fosfor İçeren Etlik Piliçlerin Tibia Kemik Özellikleri, Dayanıklılığı ve Çinko Mineralizasyonu Üzerine Etkileri

This experiment was conducted to evaluate the effects of different Zn sources and microbial phytase supplemented to low available P (aP) corn-soy diets on morphometric indices of tibiotarsi, bone strength and Zn accumulation. The experimental period lasted 42 days. A total of 875 one-day-old Ross 308 male broiler chicks were randomly assigned to seven treatment groups (positive, negative and five experimental), each with five identical subgroups of 25 birds. The positive control (PC) group was fed a diet containing adequate concentration (0.45%) of available phosphorus (aP) due to mineral premix (except zinc) and feeds; the negative control (NC) group was fed a basal diet including low concentration (0.30%) of available phosphorus (aP) due to mineral premix (except zinc) and feeds; 0.30% aP and 500 FTU phytase kg-1; 0.30% aP and 75 mg Zn-proteinate kg-1; 0.30% aP and 75 mg ZnSO4 kg-1; 75 mg Zn-proteinate and 500 FTU phytase kg-1; 75 mg ZnSO4 and 500 FTU phytase kg-1 were added to the experimental groups of phytase (PH), organic zinc (OZ), inorganic zinc (IZ), organic zinc + phytase (OZ+PH) and inorganic zinc + phytase (IZ+PH) respectively. There were no significant differences among the groups in mean tibiotarsal diaphysis diameter, thickness of the medial wall, tibiotarsal index, medullary canal diameter, modulus of elasticity or breaking stress. However, diet with OZ + PH and IZ + PH supplementation had a greater influence on tibiotarsal weight and tibiotarsal weight/length index when compared to those measurements in broilers in both control groups (P<0.001). In addition, feed additives were seen to have a significant effect on tibiotarsal length (P<0.01), robusticity index (P<0.001), thickness of the lateral wall (P<0.05) and Zn content (P<0.05). In conclusion, the study indicated that the use of organic and inorganic Zn alone or in combination with microbial phytase improved tibial bone traits and Zn content.

___

  • 1. Fordyce EJ, Forbes RM, Robbins KR, Erdman Jr JW: Phytate, calcium/ zinc molar ratios: Are they predictive of zinc bioavailability? J Food Sci, 52, 440–444, 1987. DOI: 10.1111/j.1365-2621.1987.tb06634.x
  • 2. NRC: Nutrient Requirements of Poultry. 9th ed., Natl. Acad. Press, Washington, DC, 1994.
  • 3. Burrell AL, Dozier WA, Davis AJ, Compton MM, Freeman ME, Vendrell PF, Ward TL: Responses of broilers to dietary zinc concentrations and sources in relation to environmental implications. Br Poult Sci, 45, 255-263, 2004. DOI: 10.1080/00071660410001715867
  • 4. Salim HM, Lee HR, Jo C, Lee SK, Lee BD: Effect of sources and levels of zinc on the tissue mineral concentration and carcass quality of broilers. Avian Biol Res, 3, 23-29, 2010. DOI: 10.3184/175815510X126365 95095213
  • 5. Mañón A, Cantor A, Pescatore, A, Ford M, Gillespie H, Daley M: Influence of dietary supplementation of organic minerals and phytase on mineral concentration in manure of replacement pullets. Poult Sci Assoc Ann Meeting Abstr, 84-85, 2005.
  • 6. Ammerman CB, Baker DH, Lewis AJ: Bioavailability of Nutrients for Animals: Amino Acids, Minerals and Vitamins. Academic Press, San Diego, CA, 1995.
  • 7. Pimental JL, Cook ME, Greger JL: Bioavailability of zinc methionine for chicks. Poult Sci, 70, 1637-1639, 1991. DOI: 10.3382/ps.0701637
  • 8. Wise A: Dietary factors determining the biological activities of phytase. Nutr Abstr Rev, 53, 791-806, 1993.
  • 9. Pallauf J, Rimbach G: Nutritional significance of phytic acid and phytase. Arch Anim Nutr, 50, 301-319, 1997. DOI: 10.1080/17450399709386141
  • 10. Denbow DM, Ravindran V, Kornegay ET, Yi Z, Hulet RM: Improving phosphorus availability in soybean meal for broilers by supplemental phytase. Poult Sci, 74, 1831-1842, 1995. DOI: 10.3382/ps.0741831
  • 11. Yi Z, Kornegay ET, D.M, Denbow DM: Supplemental microbial phytase improves zinc utilization in broilers. Poult Sci, 75, 540-546, 1996. DOI: 10.3382/ps.0750540
  • 12. Shelton JL, Southern LL: Effects of phytase addition with or without a trace mineral premix on growth performance, bone response variables, and tissue mineral concentration in commercial broilers. J Appl Poult Res, 15, 94-102, 2006. DOI: 10.1093/japr/15.1.94
  • 13. Rath NC, Huff GR, Huff WE, Balog JM: Factors regulating bone maturity and strength in poultry. Poult Sci, 79, 1024-1032, 2000. DOI: 10.1093/ps/79.7.1024
  • 14. Reichmann KG, Connor JK: Influence of dietary calcium and phosphorus on metabolism and production in laying hens. Br Poult Sci, 18, 633-640, 1977. DOI: 10.1080/00071667708416414
  • 15. Rowland LOJr, Harms RH, Wilson, HR, Ross, IJ, Fry JL: Breaking strength of chick bones as an indication of dietary calcium and phosphorus adequacy. Proc Soc Exp Biol Med, 126, 399-401, 1967. DOI: 10.3181/00379727-126-32458
  • 16. Onyango EM, Hester PY, Stroshine R, Adeola O: Bone densitometry as an indicator of percentage tibia ash in broiler chicks fed varying dietary calcium and phosphorus levels. Poult Sci, 82, 1787-1791, 2003. DOI: 10.1093/ps/82.11.1787
  • 17. Barnet E, Nordin B: The radiological diagnosis of osteoporosis: A new approach. Clin Radiol, 11, 166-169, 1960. DOI: 10.1016/S0009- 9260(60)80012-8
  • 18. Virtama P, Telkka A: Cortical thickness as an estimate of mineral content of human humerus and femur. Bt J Radiol, 35, 623-625, 1962. DOI: 10.1259/0007-1285-35-417-632
  • 19. Reisenfeld A: Metatarsal robusticity in bipedal rats. Am J Phys Anthropol, 40, 229-234, 1972. DOI: 10.1002/ajpa.1330360211
  • 20. Seedor JG, Quarruccio HA, Thompson DD: The bisphosphonate alendronate (MK-217) inhibits bone loss due to ovariectomy in rats. J Bone Miner Res, 6, 339-346, 1991. DOI: 10.1359/jbmr.2005.20.2.354
  • 21. Lott BD, Reece FN, Drott JH: Effect of preconditioning on bone breaking strength. Poult Sci, 59, 724-725, 1980. DOI: 10.3382/ps.0590724
  • 22. Patterson PH, Cook ME, Crenshaw TD, Sunde ML: Mechanical properties of the tibiotarsus of broilers and poults loaded with artificial weight and fed various dietary protein levels. Poultry Sci, 65, 1357-1364, 1986. DOI: 10.3382/ps.0651357
  • 23. Midilli M, Salman M, Muğlalı ÖH, Öğretmen T, Çenesiz S, Ormancı N: The effects of organic or ınorganic zinc and microbial phytase, alone or in combination, on the performance, biochemical parameters and nutrient utilization of broilers fed a diet low in available phosphorus. Kafkas Univ Vet Fak Derg, 20, 89-96, 2014. DOI: 10.9775/kvfd.2013.9497
  • 24. Carpenter KJ, Clegg KM: The metabolizable energy of poultry feeding stuffs in relation to their chemical composition. J Sci Food Agric, 7, 45-51, 1956. DOI: 10.1002/jsfa.2740070109
  • 25. Timoshenko SP, Goodier JN: The Theory of Elasticity. McGraw-Hill. New York, 113-122, 1987.
  • 26. AOAC: Official Methods of Analysis. 17th ed., Association of Official Analytical Chemists, Arlington, VA. 2003.
  • 27. SPSS: SPSS for Windows, Standard Version 10.0.1, SPSS Inc., Headquarters, Chicago, Illinois. 2005.
  • 28. Duncan DB: Multiple Range and Multiple F Tests. Biometrics, 11, 1-42, 1955.
  • 29. Qian H, Veit HP, Kornegay ET, Ravindran V, Denbow DM: Effects of supplemental phytase and phosphorus on histological and other tibial bone characteristic and performances of broilers fed semi-purified diets. Poult Sci, 75, 618-626, 1996. DOI: 10.3382/ps.0750618
  • 30. Sahraei M, Janmmohamdi H, Taghizadeh A, Cheraghi S: Effect of different zinc sources on tibia bone morphology and ash content of broiler chickens. Adv Biol Res, 6, 128-132, 2012.
  • 31. Monteagudo MD, Hernandz ER, Seco C, Gonzales Riola J, Revilla M, Villa LF, Rico H: Comparison of the bone robusticity index and bone weight/bone length index with the results of bone densitometry and bone histomorphometry in experimental studies. Acta Anat, 160, 195- 199, 1997. DOI: 10.1159/000148011
  • 32. Kocabagli N: The effect of dietary phytase supplementation at different levels on tibial bone characteristics and strength in broilers. Turk J Vet Anim Sci, 25, 797-802, 2001.
  • 33. Perney KM, Cantor AH, Straw ML, Herkelman KL: The effect of dietary phytase on growth performance and phosphorus utilization of broiler chicks. Poult Sci, 72, 2106-2114, 1993. DOI: 10.3382/ps.0722106
  • 34. Sohail SS, Roland DA: Influence of supplemental phytase on performance of broilers four to six weeks of age. Poult Sci, 78, 550-555, 1999. DOI: 10.1093/ps/78.4.550
  • 35. Shelton JL, Southern LL: Interactive effect of zinc, copper and manganese in diets for broilers. Int J Poult Sci, 6, 466-469, 2007. DOI: 10.3923/ijps.2007.466.469
  • 36. Scrimgeour AG, Stahl CHH, McClung JP, Marchitelli LJ, Young AJ: Moderate zinc deficiency negatively affects biomechanical properties of tibiae independently of body composition. J Nutr Bioch, 18, 813-819, 2007. DOI: 10.1016/j.jnutbio.2006.12.018
  • 37. Jongbloed A, Kemme P, De Grootte G, Lippens M and Meschy F: Bioavailability of major and trace elements, EMFEMA (International Association of the European Manufacturers of Major, Trace and Specific Feed Mineral Materials), Brussels, pp.118, 2002.
  • 38. Underwood EJ, Suttle NF: The Mineral Nutrition of Livestock. 3rd ed., CABI Publishing, Biddles Ltd., Guilford and King’s Lynn, UK, 477-512, 1999.
  • 39. Roberson KD, Edwards Jr HM: Effects of 1,25-dihydroxycholecalciferol and phytase on zinc utilization in broiler diets. Poult Sci, 73, 1312-1326, 1994. DOI: 10.3382/ps.0731312
  • 40. Mohanna C, Carré B, Nys Y: Incidence of dietary viscosity on growth performance and zinc and manganese bioavailability in broilers. Anim Feed Sci Technol, 77, 255-266, 1999. DOI: 10.1016/S0377- 8401(98)00256-9
  • 41. Ao T, Pierce JL, Power R, Dawson KA, Pescatore AJ, Cantor AH, Ford MJ: Evaluation of bioplex Zn as an organic zinc source for chicks. Int J Poult Sci, 5, 808-811, 2006. DOI: 10.3923/ijps.2006.808.811
  • 42. Aksu T, Özsoy B, Aksu DS, Yörük MA, Gül M: The effects of lower levels of organically complexed zinc,copper and manganese in broiler diets on performance, mineralconcentration of tibia and mineral excretion. Kafkas Univ Vet Fak Derg,17, 141-146, 2011. DOI: 10.9775/ kvfd.2010.2735
Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
Sayıdaki Diğer Makaleler

Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]

DENİZ DİNÇEL, SENA ARDIÇLI, Bahadır SOYUDAL, Mehlika ER, FAZLI ALPAY, HALE ŞAMLI, Faruk BALCI

The Effects of High and Low Altitudes on Conjunctival Flora in Sport and Work Horses: A Field Study in the Northeast Anatolia Region of Turkey (Kars and Iğdır)

VEDAT BARAN, İSA ÖZAYDIN, OKTAY GENÇ, SALİH OTLU, Sadık YAYLA, ENGİN KILIÇ, ÖZGÜR ÇELEBİ, Uğur AYDIN, ELİF ÇELİK, ALİYE GÜLMEZ SAĞLAM

Effect of Met-Anandamide on Prevention of Hyperactivation, Cryo-Capacitation and Acrosome Reaction in Ram Semen Cryopreservation

Reza TALEBIYAN, Fardin AMIDI, Morteza SAMINI, Pezhman MIRSHOKRAEI, Saeid HABIBIAN DEHKORDI

Genetic Polymorphism of Five Genes Associated with Meat Production Traits in Five Cattle Breeds in Turkey

Özgecan AĞAOĞLU KORKMAZ, BİLAL AKYÜZ, BENGİ ÇINAR KUL, Nüket BİLGEN, Okan ERTUĞRUL

Determination of Aflatoxin Levels in Maize Grain by High Performance Liquid Chromatography Using an Immunoaffinity Column Cleanup

ENES ATMACA, DİLEK GÜVENÇ, ABDURRAHMAN AKSOY

Protection and Efficacy of Cell Culture Propagated Montanide Adjuvant Based Inactivated Vaccine Against Hydropericardium Syndrome in Poultry

Asma JABEEN, Khalid NAEEM, Naila SIDDIQUE, Muhammad Athar ABBAS, Akbar ALI, Syed Muhammad Saqlan NAQVI

The Effects of Different Zinc Sources and Microbial Phytase Supplementation on the Tibial Bone Properties, Strength and Zn Mineralization Broilers Fed with Diet Low Phosphorus [1]

MUSTAFA MİDİLLİ, Mustafa SALMAN, Ömer Hakan MUĞLALI, SENA ÇENESİZ, Neslihan ORMANCI, MURAT PAKDİL, İ. Safa GÜRCAN

Effect of Different Housing Systems on Production and Blood Profile of Slow-Growing Broilers

Merve DİKTAŞ, AHMET ŞEKEROĞLU, MUSTAFA DUMAN, ARDA YILDIRIM

The Influence of Body Weight on Carcass and Carcass Part Yields, and Some Meat Quality Traits in Fast- and Slow-Growing Broiler Chickens

Doğan NARİNÇ, TÜLİN AKSOY, ALPER ÖNENÇ, Deniz ÇÜREK İLASLAN

The Comparative Analysis of Phenotypic and Genotypic Properties of Aeromonas sobria Strains Isolated from Rainbow Trout (Oncorhynchus mykiss, Walbaum, 1972)

ALPER ÇİFTCİ, ERTAN EMEK ONUK, GÜLAY ÇİFTCİ, ARZU FINDIK, Mehtap Ünlü SÖĞÜT, TİMUR GÜLHAN