ALPER ÇİFTCİ, ERTAN EMEK ONUK, GÜLAY ÇİFTCİ, ARZU FINDIK, Mehtap Ünlü SÖĞÜT, TİMUR GÜLHAN

The Comparative Analysis of Phenotypic and Genotypic Properties of Aeromonas sobria Strains Isolated from Rainbow Trout (Oncorhynchus mykiss, Walbaum, 1972)

Bu araştırmada Aeromonas sobria (A. sobria) izolatlarının antibiyotiplendirme, tüm hücre proteinlerinin sodyum dodesilsulfat-poliakrilamid jel electroforezi (SDS-PAGE) ile analizi, rastgele çoğaltılmış polimorfik DNA (RAPD) PCR ile genotiplendirme ile fenotipik ve genotipik karakterizasyonu amaçlanmıştır. Bu amaçla otuzaltı adet A. sobria izolatı incelenmiştir. İzolatlar antibiyotik duyarlılık sonuçlarına göre 12 farklı antibiyotip ve SDS-PAGE profiline göre 4 farklı proteotipe sahip oldukları belirlenmiştir. İzolatların 13 farklı genotipe sahip oldukları saptanmıştır. Sonuç olarak, Türkiye izolatı A. sobria suşlarının fenotipik ve genotipik olarak çeşitlilik gösterdiği ve çalışmada kullanılan tiplendirme metodlarının ikili veya üçlü kombinasyonlar halinde kullanılmasının suş ayrımında daha faydalı olduğu belirlenmiştir. Çalışmada elde edilen sonuçların balıklarda A. sobria infeksiyonlarının antibiyotik tedavi seçeneklerinin belirlenmesi konusunda faydalı olmakla birlikte, konu ile ilgili olarak yapılacak olan aşı ve teşhis kiti geliştirilmesi çalışmalarına öncülük edeceği düşünülmektedir.

Gökkuşağı Alabalıklarından (Oncorhynchus mykiss, Walbaum, 1972) İzole Edilen Aeromonas sobria Suşlarının Fenotip ve Genotip Yönünden Karşılaştırmalı Analizi

Phenotypic and molecular characterization of Aeromonas sobria (A. sobria) isolates by antibiotyping, sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of whole cell proteins, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was aimed in this study. For this aim, thirty-six A. sobria isolates were analysed. Isolates were divided into 12 different antibiotypes and 4 proteotypes according to their antibiotic susceptibilities and SDS-PAGE patterns, respectively. Thirteen RAPD types were observed among all isolates. In conclusion, the use of double or triple combination of typing methods in this study was found to be more useful for discriminating the strains. The results obtained from this study may give information about phenotypic and genotypic variability of the A. sobria strains isolated from different regions of Turkey and can be helpful to control disease in fish through guiding the antibiotic therapy and giving information that will be useful to development vaccine.

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