The Effect of Testosterone Supplementation on Capillar Morphometry and VEGF Expression Level of the Heart in Aged Mice

The aim of this study was to determine the effct of testosterone hormone supplementation on the capillary morphometry and VEGF expression level of the heart of old mice using histological and molecular techniques. A total of 30 old mice were enrolled in 3 groups: sham operation group (Control), gonadectomy group (G) and gonadectomy and testosterone supplementation group (GTS). The capillary number and the inner diameter of larger capillary vessels in the heart were measured by light microscope in terms of angiogenic processes. The levels of angiogenic factor, VEGF (vascular endothelial growth factor), mRNA in the heart tissue were determined by RT-PCR. The capillary densities decreased in female mice, in which G and GTS (P

Testosteron Takviyesinin Yaşlı Farelerin Kalbinde Kapillar Morfometri ve VEGF Expresyon Seviyesine Etkisi

Bu çalışmanın amacı yaşlı farelerin kalbinde testosteron hormon takviyesinin anjiyogenik etkisini moleküler ve histolojik olarak tespit etmektir. Toplam 30 fareden 3 çalışma grubu oluşturulmuştur. Bunlar; yalancı operasyon yapılan (kontrol) grup, gonadektomi yapılan (G) grup ve hem gonadektomi hem de testosteron takviyesi yapılan (GTS) gruptur. Kalp dokusunda kapillar sayısı ve geniş kapillar damarların iç çapı ışık mikroskobunda ölçülmüştür. Kalp dokusundaki VEGF (vasküler endoteliyal büyüme faktörü) mRNA seviyesi RT-PCR ile ölçülmüştür. Dişi farelerde; kontrol grubuna göre G ve GTS (P

___

1. Otrock ZK, Mahfouz RAR, Makarem JA, Shamseddine AI: Understanding the biology of angiogenesis: Review of the most important molecular mechanisms. Blood Cells Mol Dis, 39, 212-220, 2007. DOI: 10.1016/j.bcmd.2007.04.001

2. Cai J, Hong Y, Weng C, Tan C, Imperato-McGinley J, Zhu YS: Androgen stimulates endothelial cell proliferation via an androgen receptor/VEGF/ cyclin A-mediated mechanism. Am J Physiol Heart Circ Physiol, 300, 1210- 1221, 2011. DOI: 10.1152/ajpheart.01210.2010

3. Castillo C, Cruzado M, Ariznavarreta C, Lahera V, Cachofeiro V, Loyzaga PG, Tresguerres JAF: Effects of ovariectomy and growth hormone administration on body composition and vascular function and structure in old female rats. Biogerontology, 6, 49-60, 2005. DOI: 10.1007/ s10522-004-7383-x

4. Albrecht ED, Babischkin JS, Lidor Y, Anderson LD, Udof LC, Pepe GJ: Effct of estrogen on angiogenesis in co-cultures of human endometrial cells and microvascular endothelial cells. Hum Reprod, 18, 2039-2047, 2003. DOI: 10.1093/humrep/deg415

5. Jesmin S, Hattori Y, Sakuma I, Liu MY, Mowa CN, Kitabatake A: Estrogen deprivation and replacement modulate cerebral capillary density with vascular expression of angiogenic molecules in middle-aged female rats. J Cereb Blood Flow Metab, 23, 181-189, 2003. DOI: 10.1097/01.WCB. 0000043341.09081.37

6. Soares R, Balogh G, Guo S, Rtner FG, Russo J, Schmitt F: Evidence for the notch signaling pathway on the role of estrogen in angiogenesis. Mol Endocrinol, 18, 2333-2343, 2004. DOI: 10.1210/me.2003-0362

7. Shidaifat F, Gharaibeh M, Bani-Ismail Z: Effct of castration on extracellular matrix remodeling and angiogenesis of the prostate gland. Endocr J, 54, 521-529, 2007. DOI: 10.1507/endocrj.K07-009

8. Sieveking DP, Lim P, Chow RWY, Dunn LL, Bao S, McGrath KCY, Heather AK, Handelsman DJ, Celermajer DS, Ng MKC: A sex-specific role for androgens in angiogenesis. J Exp Med, 207, 345-352, 2010. DOI: 10.1084/jem.20091924

9. Dabanoğlu İ, Bozdoğan B, Kum Ş, Ünübol Aypak S: The Angiogenic effct of testosterone supplementation on brain of aged mice. Kafkas Univ Vet Fak Derg, 23, 247-251, 2017. DOI: 10.9775/kvfd.2016.16364

10. Chodari L, Dariushnejad H, Ghorbanzadeh V: Voluntary wheel running and testosterone replacement increases heart angiogenesis through miR-132 in castrated diabetic rats. Physiol Int, 106, 48-58, 2019. DOI: 10.1556/2060.106.2019.06

11. Lam YT, Lecce L, Yuen SC, Wise SG, Handelsman DJ, Karas RH, Ng MKC: Androgens ameliorate impaired ischemia-induced neovascularization due to aging in male mice. Endocrinology, 160, 1137- 1149, 2019. DOI: 10.1210/en.2018-00951

12. Hanke H, Lenz C, Hess B, Spindler KD, Weidemann W: Effct of testosterone on plaque development and androgen receptor expression in the arterial vessel wall. Circulation, 103, 1382-1385, 2001. DOI: 10.1161/ 01.CIR.103.10.1382

13. Alexandersen P, Christiansen C: The aging male: Testosterone deficiency and testosterone replacement. An up-date. Atherosclerosis, 173, 157-169, 2004. DOI: 10.1016/S0021-9150(03)00242-9

14. Iliescu R, Reckelhof JF: Testosterone and vascular reactivity. Clin Sci, 111, 251-252, 2006. DOI: 10.1042/CS20060102

15. Reed MJ, Edelberg JM: Impaired angiogenesis in the aged. Sci Aging Knowl Environ, 2004 (7): pe7, 2004. DOI: 10.1126/sageke.2004.7.pe7

16. Reed MJ, Bradshaw AD, Shaw M, Sadoun E, Han N, Ferara N, Funk S, Puolakkainen P, Sage EH: Enhanced angiogenesis characteristic of sparc-null mice disappears with age. J Cell Physiol, 204, 800-807, 2005. DOI: 10.1002/jcp.20348

17. Sweat RS, Sloas DC, Stewart SA, Czarny-Ratajczak M, Baddoo M, Eastwood JR, Suarez-Martinez AD, Azimi MS, Burks HE, Chedister LO, Myers L, Murfee WL: Aging is associated with impaired angiogenesis, but normal microvascular network structure, in the rat mesentery. Am J Physiol Heart Circ Physiol, 312, H275-H284, 2017. DOI: 10.1152/ ajpheart.00200.2016

18. Yıldız O: Vascular smooth muscle and endothelial functions in aging. Ann N Y Acad Sci, 1100, 353-360, 2007. DOI: 10.1196/annals.1395.038

19. Hsu YJ, Dimke H, Schoeber JP, Hsu SC, Lin SH, Chu P, Hoenderop JG, Bindels RJM: Testosterone increases urinary calcium excretion and inhibits expression of renal calcium transport proteins. Kidney Int, 77, 601- 608, 2010. DOI: 10.1038/ki.2009.522

20. Crossman G: A modification of Mallory’s connective tissue stain with a discussion of the principles involved. Anat Rec, 69, 33-38, 1937. DOI: 10.1002/ar.1090690105

21. Fadini GP, Albiero M, Cignarella A, Bolego C, Pinna C, Boscaro E, Pagnin E, De Toni R, de Kreutzenberg S, Agostini C, Avogaro A: Effcts of androgens on endothelial progenitor cells in vitro and in vivo. Clin Sci, 117, 355-364, 2009. DOI: 10.1042/CS20090077

22. Chen Y, Jin X, Zeng Z, Liu W, Wang B, Wang H: Estrogen-replacement therapy promotes angiogenesis after acute myocardial infarction by enhancing SDF-1 and estrogen receptor expression. Microvasc Res, 77, 71-77, 2009. DOI: 10.1016/j.mvr.2008.10.003

23. Chen Y, Fu L, Han Y, Teng Y, Sun J, Xie R, Cao J: Testosterone replacement therapy promotes angiogenesis after acute myocardial infarction by enhancing expression of cytokines HIF-1a, SDF-1a and VEGF. Eur J Pharmacol, 684, 116-124, 2012. DOI: 10.1016/j.ejphar.2012.03.032

24. Chodari L, Mohammadi M, Ghorbanzadeh V, Dariushnejad H, Mohaddes G: Testosterone and voluntary exercise promote angiogenesis in hearts of rats with diabetes by enhancing expression of VEGF-A and SDF-1a. Can J Diabetes, 40 (5): 436-441, 2016. DOI: 10.1016/j. jcjd.2016.03.004

25. Kang NN, Fu L, Xu J, Han Y, Cao JX, Sun JF, Zheng M: Testosterone improves cardiac function and alters angiotensin II receptors in isoproterenol-induced heart failure. Arch Cardiovasc Dis, 105, 68-76, 2012. DOI: 10.1016/j.acvd.2011.12.002

26. Crafts TD, Jensen AR, Blocher-Smith EC, Markel TA: Vascular endothelial growth factor: Therapeutic possibilities and challenges for the treatment of ischemia. Cytokine, 71, 385-393, 2015. DOI: 10.1016/j. cyto.2014.08.005

27. Pongkan W, Chattipakorn SC, Chattipakorn N: Chronic testosterone replacement exerts cardioprotection against cardiac ischemia-reperfusion injury by attenuating mitochondrial dysfunction in testosterone-deprived rats. PLoS One, 10 (3): e0122503, 2015. DOI: 10.1371/journal.pone.0122503

28. Svensson J, Movérare-Skrtic S, Windahl S, Swanson C, Sjögren K: Stimulation of both estrogen and androgen receptors maintains skeletal muscle mass in gonadectomized male mice but mainly via diffrent pathways. J Mol Endocrinol, 45, 45-57, 2010. DOI: 10.1677/JME-09-0165

29. Ruohola JK, Valve EM, Karkkainen MJ, Joukov V, Alitalo K, Härkönen PL: Vascular endothelial growth factors are differentially regulated by steroid hormones and antiestrogens in breast cancer cells. Mol Cell Endocrinol, 149, 29-40, 1999. DOI: 10.1016/S0303-7207(99)00003-9

30. Rodness J, Mihic A, Miyagi Y, Wu J, Weisel RD, Li RK: VEGF-loaded microsphere patch for local protein delivery to the ischemic heart. Acta Biomater, 45, 169-181, 2016. DOI: 10.1016/j.actbio.2016.09.009

31. Goodale T, Sadhu A, Petak S, Robbins R: Testosterone and the heart. Methodist Debakey Cardiovasc J, 13, 68-72, 2017.

32. Oduk Y, Zhu W, Kannappan R, Zhao M, Borovjagin AV, Oparil S, Zhang JJ: VEGF nanoparticles repair the heart after myocardial infarction. Am J Physiol Heart Circ Physiol, 314, H278-H284, 2018. DOI: 10.1152/ ajpheart.00471.2017
Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
Sayıdaki Diğer Makaleler

Yitirilen Bir Servet: Tiftik Keçisi

Şule SANAL, Ayşe MENTEŞ GÜRLER1, MEHMET YAVUZ ERLER

Diagnosis of Multiple Congenital Cardiac Defects in a Newborn Calf

MERİÇ KOCATÜRK, Volkan YAZICIOĞLU, HAKAN SALCI, ERSOY BAYDAR, ZEKİ YILMAZ

Sokak Köpeklerinde Spesifik Olmayan Reaktif Hepatit Çalışması

Danilo SUAREZ, David FARRAY, Jose PEREZ, Myriam RODRIGUEZ-VENTURA, Alicia VELAZQUEZ-WALLRAF, Jose Raduan JABER, Antonio RAVELO-GARCÍA, Conrado CARRASCOSA

Mandibular Hypertrophic Osteodystrophy Fibrosa in a German Shepherd Puppy

Elham HASSAN, Faisal TORAD, Ashraf ABU-SEIDA, Azza HASSAN

Serum Thiol Disulphide Levels Among Sheep with Sarcoptic Mange

İLKER ÇAMKERTEN, Güzin ÇAMKERTEN, HASAN ERDOĞAN, ADNAN AYAN, Songül ERDOĞAN, KEREM URAL

Türkiye’deki Alaca Atların mtDNA D-Loop Dizi Analizi İle Genetik Yapılarının Araştırılması

Abdurrahman KOSEMAN, Yusuf OZSENSOY, Kemal ZENGİN, Cevdet YARALI, İbrahim SEKER, Metin ERDOGAN, Burhan TOPRAK

PCA Destekli J48 Algoritması İle Manyetik Alanla Tedavi Edilen Diyabetik Sıçanların Biyokimyasal ve Biyomekanik Verilerinin Sınıflndırılması

Bora TAŞTEKİN, Turgay İBRİKÇİ, Aykut PELİT, Pelin ÖZALP

Derin Kızartılmış Ayçiçeği Yağının Bir Fare Modelinde Sperm Parametreleri Üzerindeki Etkileri: Probiyotiklerin Koruyucu Etkisi Var mı?

Zafer SABİT, Nurhayat GÜLMEZ, Serkan SAYINER, Murat GÜLMEZ

Effects of High Rice Diet on Growth Performance, Nutrients Apparent Digestibility, Nitrogen Metabolism, Blood Parameters and Rumen Fermentation in Growing Goats

Kaijun WANG, Mengli ZHENG, Ao REN, Chuanshe ZHOU, Qiongxian YAN, Zhiliang TAN, Peihua ZHANG, Kangle YI

Büyüme Dönemindeki Keçilerde Pirinç Ağırlıklı Diyetin Büyüme Performansı, Görünür Sindirilebilirlik, Nitrojen Metabolizması, Kan Parametreleri ve Rumen Fermentasyonu Üzerine Etkileri

Mengli ZHENG, Ao REN, Zhiliang TAN, Peihua ZHANG, Kaijun WANG, Qiongxian YAN, Kangle YI, Chuanshe ZHOU