MUSTAFA YILDIZ, IŞIL AYDEMİR, ŞADİYE KUM, ÜLKER EREN

The Distribution and Heterogeneity of Mast Cells in the Cecum of Quail (Coturnix Coturnix Japonica)

Sunulan çalışmanın amacı, bıldırcın sekumunda mast hücrelerinin yerleşimini ve heterojenitesini araştırmaktır. Sekum örnekleri bazik kurşun asetat (BLA), Carnoy, izotonik formaldehit asetik asit (IFAA) ve %10'luk nötr tamponlu formalin (NBF) solüsyonlarında tespit edildi. Kesitler alsiyan mavisi-kritik elektrolit konsantrasyonu (AB-CEC) (pH 5.8, 0.3 M MgCl2)/safranin O (SO) (pH 1.0) kombine metodu ve toluidin mavisi (TB) (pH 0.5) ile boyandı. Mast hücresi populasyonu, TB ile boyanmış kesitlerde Carnoy solüsyonu ile tespit edilen sekum bölümlerinin bütün katmanlarında en yüksek oranlarda gösterildi. Metakromatik mast hücresi yoğunluğu en fazla orta ve distal sekumun tunika mukoza katmanlarında belirlendi. AB-CEC (+)/SO (-) mast hücresi yoğunluğunun ise, Carnoy tespitine göre IFAA ve BLA ile tespit edilen dokularda, proksimal ve orta sekumun tunika mukoza katmanlarında daha fazla olduğu görüldü. Araştırma sonunda; bıldırcın sekumunda Carnoy tespit solüsyonunun bağ doku mast hücrelerini, IFAA ve BLA tespit solüsyonlarının ise mukozal mast hücrelerini daha iyi tespit ettiği söylenebilir.

Bıldırcın (Coturnix Coturnix Japonica) Sekumunda Mast Hücrelerinin Dağılımı ve Heterojenitesi

The aim of the present study is to investigate the location and heterogeneity of mast cells in quail cecum. Cecum samples were fixed in basic lead acetate (BLA), Carnoy's, isotonic formaldehyde acetic acid (IFAA) and 10% neutral buffered formalin (NBF) solutions. The sections were stained with an alcian blue-critical electrolyte concentration (AB-CEC) (pH 5.8, 0.3 M MgCl2) and safranin O (SO) (pH 1.0) used in a combined method and with toluidine blue (TB) (pH 0.5). Mast-cell population was shown in highest ratios in all layers of Carnoy fixed cecum parts within TB-stained sections. Metachromatic mast cell density was determined the most in tunica mucosa layers of the middle and distal cecum. It was seen that AB-CEC (+)/SO (-) mast-cell density was greater in tunica mucosa layers of the proximal and middle cecum in IFAA and BLA fixed tissues campare to Carnoy fixation. At the end of the study; it can be said that Carnoy solution fixed the connective tissue mast cells, as well as IFAA and BLA solutions fixed the mucosal mast cells better.

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