Dimetilsülfoksit İlavesi ile Farklı Şekillerde Dondurulmuş Rumen Sıvısının in Vitro Sindirim Denemelerinde Kullanım Olanaklarının Araştırılması

Bu çalışmada, rumen sıvısı kaynağı olarak; taze rumen sıvısı, sıvı azot içerisinde dimetilsülfoksit (DMSO) katkısız ve katkılı (%5), derin dondurucuda DMSO katkısız ve katkılı (%5) dondurulmuş rumen sıvısı kullanılmıştır. Çalışmada 4 farklı kaba yem (mısır silajı, yonca kuru otu, mercimek samanı, buğday samanı), 4 farklı karma yem (sığır besi yemi, sığır süt yemi, toklu besi yemi ve buzağı büyütme yemi) ve 4 farklı yem hammaddesi (arpa, mısır, pamuk tohumu küspesi, soya küspesi) yem materyali olarak kullanılmıştır. İki aşamalı sindirim yönteminde deneme yemleri genel olarak değerlendirildiğinde gerek sıvı azot içerisinde ve gerekse derin dondurucuda dondurulan rumen sıvılarından elde edilen in vitro organik madde sindirim (İVOMS) değerleri taze rumen sıvısından elde edilmiş değerlerden düşük (P0.05) bulunmuştur. Araştırmada değerlendirilen tüm dondurulmuş rumen sıvılarındaki toplam anaerob bakteri ve toplam protozoa sayılarının, taze rumen sıvısındaki toplam anaerob bakteri ve protozoa sayılarına kıyasla azaldığı (P0.05) olduğu belirlenmiştir. Bu araştırmadan elde edilen sonuçlara göre, %5 DMSO katkısı ile sıvı azot tankı içerisinde dondurulmuş rumen sıvılarının in vitro gaz üretim tekniğinde kullanılabileceği sonucuna varılmıştır.

An Investigation on Usage of Frozen Rumen Fluid with Adding Dimethylsulfoxide and Different Freezing Methods for Determination of in Vitro Digestibility

In this study, fresh rumen fluid, liquid nitrogen frozen rumen fluids with addition of 5% dimethylsulfoxide (DMSO) or without DMSO, deep freeze frozen rumen fluid addition of 5% DMSO or without DMSO were investigated as inoculum sources. Four roughages (corn silage, alfalfa hay, lentil straw, wheat straw), four commercial feeds (beef finishing, dairy cattle, ram lamb finishing, calf growing feeds) and four feed ingredients (barley, corn, cotton seed meal, soybean meal) were chosen as feed materials. Values of in vitro organic matter digestibility (IVOMD) of feeds using fresh rumen fluid were found generally higher than the values obtained from nitrogen or deep freeze frozen inoculum sources (P<0.05) in two stage in vitro procedure. Values of IVOMD via gas production technique were similar (P>0.05) with using liquid nitrogen frozen rumen fluids with addition of 5% DMSO. Number of the anaerobic bacteria and total protozoa were diminished with both freezing methods comparison with fresh rumen fluid (P<0.05). Species of protozoa count of fresh rumen fluid and nitrogen frozen with 5% DMSO were found generally similar (P>0.05). According to result of this study liquid nitrogen frozen rumen fluid with addition of 5% DMSO can be suggest as an alternative inoculum source for the in vitro gas production technique.

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