The determination of N-acetylneuraminic acid (Neu5Ac) and N-glycolyl-neuraminic acid (Neu5Gc) types of sialic acids in hematopoietic organ of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae)

Hücre yüzey glikolipit ve glikoproteinlerin en uç kısımlarında yer alan ve özellikle hücre ilişkilerinde ve sinyal iletimi olaylarında önemli rolleri olan sialik asitler, uzun zamandır böceklerde olmadığı kabul edilirdi. Bu çalışmada, ipekböceği Bombyx mori larvalarının hematopoietik organında çoğu organizmada en çok bilinen Neu5Ac ve Neu5Gc tip sialik asitlerin bulunduğu tespit edilmiştir. FITC- ve altın bağlanmış lektinler (Limulus polyphemus, LPA) kullanılarak hematopoietik organ kesitlerinde histokimyasal ve sitokimyasal yöntemlerle sialik asitlerin varlığı gösterildi. Bu sonuçlar böceklerde sialik asit bulunduğuna dair ek bir kanıt oldu.

Anahtar Kelimeler:

Bombyx mori, Limulus, glikolipidler, glikoprotein, neuraminik asit, siyalik asit, ipek böceği

İpekböceği, Bombyx mori L. (Lepidoptera: Bombycidae)’nin hematopoietik organında N-asetilneuraminik asit (Neu5Ac) ve N-glikolilneuraminik asit (Neu5Gc) tip sialik asitlerinin belirlenmesi

In insects, for a long time, it was generally accepted that members of insect do not have sialic acids which are typical terminal glycans of cells surface glycolipids and glycoproteins, and thus especially important in cell interactions and signaling events. Here, it was reported the occurrence of sialic acid known the most common in all organisms (Neu5Ac and Neu5Gc) in hematopoietic organ of larvae of the silkworm, Bombyx mori. Histochemical and cytochemical analysis of hematopeietic organ sections with the FITC- and Gold-conjugated lectins (Limulus polyphemus, LPA) showed the presence of sialic acids in hematopoietic organ. The results provide further evidence for the existence of sialic acids in insects.

Keywords:

Bombyx mori, Limulus, glycolipids, glycoproteins, neuraminic acid, sialic acids, silkworms,

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