F. Azize Budak YILDIRAN, Şükran ÇAKIR

Analysis of genetic polymorphism with microsatellite method in Turkey Local Sheep Breeds

Bu çalışmada, Türkiye’nin Bafra, İvesi, Karayaka, Morkaraman, Sakız ve Türk Merinosu olarak adlandırılan 6 yerli koyun ırkında ırk içi ve ırklar arasındaki genetik ilişki mikrosatellit analizi ile belirlendi. Tüm ırklar OarCP34, MAF65, MAF209 and DYMS1 lokusları için ABI PRISM 310 sekans cihazı kullanılarak tarandı. Elde edilen bütün veriler “Genetix 4.05” ve “Populations 1.0” istatistik programları kullanılarak değerlendirildi. Türk Merinosu ırkının en yüksek heterozigotluğa sahip olduğu belirlendi. En kısa genetik mesafe Sakız ve Morkaraman ırkları arasında gözlendi ve en uzak genetik mesafe ise Türk Merinosu ve İvesi ırkları arasında gözlendi. Sonuç olarak, bu çalışmada Bafra, Sakız, Karayaka, İvesi ve Türk Merinosu isimli Türkiye yerli koyun ırklarında mikrosatellit belirleyicileri kullanılarak genetik karakterizasyon yapıldı.

Anahtar Kelimeler:

İvesi, Karayaka, Türk Merinosu, ırk farklılıkları, genetik analizler, genetik uzaklık, genetik belirleyiciler, genetik polimorfizm, heterozigotluk, lokuslar, metodoloji, mikrosatellitler, yerli çiftlik hayvanları, nükleotit dizileri, filogenetik, uydu DNA, koyun, koyun ırkları, koyun yetiştiriciliği, Morkaraman

Türkiye yerli koyun ırklarında genetik polimorfizmin mikrosatelit yöntemi ile analizi

In this study, genetic relationships within and between the sheep breeds of Turkey called Bafra, İvesi, Karayaka, Morkaraman, Sakız and Turkish Merino was determined by microsatellite method. All breeds were screened for OarCP34, MAF65, MAF209 and DYMS1 loci by using ABI PRISM 310 sequencer. The obtained data were evaluated by using “Genetix 4.05” and “ Populations 1.0” statistics programs. It was determined that the Turkish Merino breed had the highest heterozygosity. The shortest genetic distance was found between Sakız and Morkaraman breeds and the longest genetic distance was observed between Turkish Merinos and İvesi sheep breeds. As a result, in this study, genetic characterization in Turkey Local Sheep Breeds called Bafra, Sakız, Karayaka, İvesi and Turkish Merino breeds were made by using microsatellite markers.

Keywords:

Awassi, Karayaka, Turkish Merino, breed differences, genetic analysis, genetic distance, genetic markers, genetic polymorphism, heterozygosity, loci, methodology, microsatellites, native livestock, nucleotide sequences, phylogenetics, satellite DNA, sheep, sheep breeds, sheep farming, Morkaraman,

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