Analysis of genetic polymorphism with microsatellite method in Turkey Local Sheep Breeds

Bu çalışmada, Türkiye’nin Bafra, İvesi, Karayaka, Morkaraman, Sakız ve Türk Merinosu olarak adlandırılan 6 yerli koyun ırkında ırk içi ve ırklar arasındaki genetik ilişki mikrosatellit analizi ile belirlendi. Tüm ırklar OarCP34, MAF65, MAF209 and DYMS1 lokusları için ABI PRISM 310 sekans cihazı kullanılarak tarandı. Elde edilen bütün veriler “Genetix 4.05” ve “Populations 1.0” istatistik programları kullanılarak değerlendirildi. Türk Merinosu ırkının en yüksek heterozigotluğa sahip olduğu belirlendi. En kısa genetik mesafe Sakız ve Morkaraman ırkları arasında gözlendi ve en uzak genetik mesafe ise Türk Merinosu ve İvesi ırkları arasında gözlendi. Sonuç olarak, bu çalışmada Bafra, Sakız, Karayaka, İvesi ve Türk Merinosu isimli Türkiye yerli koyun ırklarında mikrosatellit belirleyicileri kullanılarak genetik karakterizasyon yapıldı.

Türkiye yerli koyun ırklarında genetik polimorfizmin mikrosatelit yöntemi ile analizi

In this study, genetic relationships within and between the sheep breeds of Turkey called Bafra, İvesi, Karayaka, Morkaraman, Sakız and Turkish Merino was determined by microsatellite method. All breeds were screened for OarCP34, MAF65, MAF209 and DYMS1 loci by using ABI PRISM 310 sequencer. The obtained data were evaluated by using “Genetix 4.05” and “ Populations 1.0” statistics programs. It was determined that the Turkish Merino breed had the highest heterozygosity. The shortest genetic distance was found between Sakız and Morkaraman breeds and the longest genetic distance was observed between Turkish Merinos and İvesi sheep breeds. As a result, in this study, genetic characterization in Turkey Local Sheep Breeds called Bafra, Sakız, Karayaka, İvesi and Turkish Merino breeds were made by using microsatellite markers.

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1. Diez-Tasco’n C, Littlejohn RP, Almeida PAR, Crawford AM: Genetic variation within the Merino sheep breed: Analysis of closely related populations using microsatellite. Animal Genetics, 31, 243-251, 2000.
2. Devrim AK, Kaya N: Genetik polimorfizm ve mikrosatellitler. Kafkas Univ Vet Fak Derg, 10 (2): 215-220, 2004.
3. Forbes SH, Hogg JT, Buchanan FC, Crawford AM, Allendor FW: Microsatellite evolution in congeneric Mammals: Domestic and bighorn sheep. Mol Bio. Evol, 12 (6): 1106-1113, 1995.
4. Baumung R, Simianer H, Hoffmann I: Genetic diversity studies in farm animals-a survey. J Anim Breed Genet, 121, 361-373, 2004.
5. Secondary Guidelines for Development of National Farm Animal Genetic Resources Management Plans. Measurement of domestic animal diversity (MoDAD): Recommended microsatellite markers. Rome. FAO. 2001. http://dad.fao.org/cgi-bin/getblob.cgi?sid=88ed1a36db578882ebc221aa8ce4f164,50006220, Accessed: 23.09.2011
6. Belkhir K, Borsa P, Chikhi L, Goudet J, Bonhomme F: Genetix 4.00 Windows software population genetics, Laboratoire Genome, Populations, Interactions, University of Montpellier, France, 1996-2000.
7. Soysal MI, Koban E, Özkan E, Altunok V, Bulut Z, Nizamlıoğlu M, Togan I: Evolutionary relation sheep among three native and two crossbreed sheep breeds of Turkey: Preliminary results. Revue Mèd Vèt, 5, 289-293, 2005.
8. Gutierrez-Gil B, Uzun M, Arranz JJ, Primitivo FS, Yıldız S, Çenesiz M, Bayón Y: Genetic diversity in Turkish sheep. Acta Agriculturae Scand Section A, 56, 1-7, 2006.
9. Uzun M, Gutierrez-Gil B, Arranz JJ, Primitivo FS, Saatçi M, Kaya M, Bayón Y: Genetic relationships among Turkish sheep. Genet Sel Evol, 38, 513-524, 2006.
10. Koban E: Genetic diversity native and crossbreed sheep breeds in Anotolia. PhD Thesis, Institute of Science, ODTU, Turkey, 2004.
11. Arranz JJ, Bayo’n Y, San Primitivo F: Genetic variation at micro-satellite loci in Spanish sheep. Small Ruminant Research, 39, 3-10, 2001.
12. Dalvit C, Sacca E, Cassandro M, Gervaso M, Pastore E, Piasentier E: Genetic characterization of Alpine sheep breeds. Acta agriculturae Slovenica, 2, 79-84, 2008.
13. Gutiérrez-Espeleta GA, Kalinowski ST, Boyce WM, Hedrick PW: Genetic variation and population structure in desert bighorn sheep: Implications for conservation. Conservation Genetics, 1, 3-15, 2000.
14. Arora R, Bhatia S: Genetic structure of Muzzafarnagri sheep based on microsatellite analysis. Small Rum Res, 54, 227-230, 2004.