Recombinant human IGF-I (insülin-like growth factor-I) enjeksiyonunun ratlarda (Wistar albino) Musculus gastrocinemius üzerine etkileri

Bu çalışma, rh IGF-I enjeksiyonunun ratların, m. gastrocnemius’u üzerine etkilerinin histolojik yöntemlerle araştırılması amacı ile yapılmıştır. Bu çalışmada deney hayvanı olarak 30 adet dişi rat 2 gruba ayrılıp kullanıldı. Deney grubundaki ratlara 7 gün süre ile rh IGF-I subkutan enjekte edildi. Enjeksiyonu takip eden 8. günde devamında 15. günde ve 22. gün de ratlar tartılarak, servikal dislokasyon yapıldı ve m. gastrocinemius kası alındı. Rh IGF-I uygulamasının canlı ağırlık artışı istatistiksel düzeyde önemli olmasa da gastrocinemius kasında hücre sayısının ve çapının artışı üzerinde olumlu etkiler yaptığı tespit edildi.

Anahtar Kelimeler:

dişi hayvanlar, doku bilimi, insulin benzeri büyüme faktörü, canlı ağırlık artışı, metodoloji, kaslar, farmakodinamik, rekombinant protein, derialtı enjeksiyon

The effects of recombinant human IGF-I injection on Musculus gastrocinemius of rats (Wistar albino)

The purpose of the present study was to investigate the effects of rh IGF-I injection on the rat m. gastrocnemius by using histological methods. Total of 30 female rats (Wistar albino) were divided into two groups. Rh IGF-I was subcutaneusly injected in to rat for 7 days. At the end of the injection period, rats were weighed and cervical dislocation was made, and then m. gastrocnemius was removed from one experiment and one control group rat for each corresponding days to between the 8th, 15th and 22th days. Histological preparations and histometrical measurements were carried out for the muscle samples. In coclusion, rh IGF-I injection increased in both diameter and number of rat gastrocnemius muscle without a statistically significant weight gain.

Keywords:

female animals, histology, insulin-like growth factor, liveweight gain, methodology, muscles, pharmacodynamics, recombinant proteins, subcutaneous injection,

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